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Biosensor enzyme sensitive film and its preparation method

A technology of biosensors and sensitive membranes, applied in biochemical equipment and methods, measurement/inspection of microorganisms, instruments, etc., can solve the problems of low sensitivity of sensors, no application of biosensors, long electrode response time, etc., and achieve improvement Sensitivity, improve overall performance, improve long-term effect

Inactive Publication Date: 2008-01-16
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This type of sensor has products on the market, but it has many disadvantages: for example, the electrode is highly dependent on the oxygen content in the solution, the diffusion rate of oxygen, and the concentration gradient, so the detection is easily affected by the fluctuation of the oxygen partial pressure in the environment; the electrode response Long time and difficult to analyze in vivo; also the sensitivity of the sensor is not very high
The response potential of this electrode to NADH is only -0.08V (vs. SCE), and the lowest detection limit of NADH is 3.9×10 -6 M, but this material has not been used in the production of biosensors

Method used

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  • Biosensor enzyme sensitive film and its preparation method
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Examples

Experimental program
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Effect test

Embodiment 1

[0048] A Preparation of methylene blue intercalated manganese dioxide supramolecular structure material by stripping and reassembling

[0049] A-1. 200mL containing 0.8mol / L NaOH and 1.2mol / L H 2 o 2 The mixed solution was quickly added to 100mL containing 0.3mol / L Mn(NO 3 ) 2 solution, vigorously stirred and reacted for 20 minutes, filtered, transferred the filter cake to a polytetrafluoroethylene container, added 50mL NaOH solution with a concentration of 2.5mol / L, stirred into a paste, sealed the polytetrafluoroethylene container in water In a hot kettle, hydrothermally treat at 150°C for 16 hours, cool the hydrothermal kettle naturally to room temperature, open the kettle for suction filtration, wash the filter cake with deionized water until the pH value of the filtrate is 8, and dry the filter cake in an air atmosphere at 70°C for 7 Hour, obtain layered manganese dioxide;

[0050] A-2. Add 2.8g of the above-mentioned layered manganese dioxide solid powder to 260mL of...

Embodiment 2

[0058] A Preparation of methylene blue intercalated manganese dioxide supramolecular structure material by stripping and reassembling

[0059] A-1. 200mL containing 0.6mol / L NaOH and 1.5mol / L H 2 o 2 The mixed solution was quickly added to 100mL containing 0.4mol / L Mn(NO 3 ) 2 solution, vigorously stirred and reacted for 30 minutes, filtered, transferred the filter cake to a polytetrafluoroethylene container, added 50mL NaOH solution with a concentration of 2mol / L, stirred into a paste, sealed the polytetrafluoroethylene container in a hydrothermal In the kettle, hydrothermally treat at 160°C for 18 hours, cool the hydrothermal kettle naturally to room temperature, open the kettle for suction filtration, wash the filter cake with deionized water until the pH value of the filtrate is 9, and dry the filter cake in an air atmosphere at 80°C for 9 hours , to obtain layered manganese dioxide;

[0060] A-2. Add 2.5g of the above layered manganese dioxide solid powder to 300mL of...

Embodiment 3

[0068] A Preparation of methylene blue intercalated manganese dioxide supramolecular structure material by stripping and reassembling

[0069] A-1. 200mL containing 0.8mol / L NaOH and 1.5mol / L H 2 o 2 The mixed solution was quickly added to 100mL containing 0.5mol / L Mn(NO 3 ) 2 solution, vigorously stirred and reacted for 25 minutes, filtered, transferred the filter cake to a polytetrafluoroethylene container, added 50mL NaOH solution with a concentration of 2.3mol / L, stirred into a paste, sealed the polytetrafluoroethylene container in water In a hot kettle, conduct hydrothermal treatment at 160°C for 15 hours, cool the hydrothermal kettle naturally to room temperature, open the kettle for suction filtration, wash the filter cake with deionized water until the pH value of the filtrate is 8.5, and dry the filter cake in an air atmosphere at 75°C for 9 Hour, obtain layered manganese dioxide;

[0070] A-2. Add 2.0g of the above-mentioned layered manganese dioxide solid powder...

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Abstract

The invention relates to a preparation method of enzyme function sensitive film comprising a methylene blue intercalation manganese dioxide supermolecular structure material and the application thereof in electrochemistry biological sensor; the methylene blue is inserted into manganese dioxide layers by the method of removal and reassembly; an ethanol dispersion liquid is dropped and smeared on the surface of a solid electrode; a homogeneous mixed liquid of a biological compatibility polymer and a biological active substance is dropped and smeared on the surface of the solid electrode after the ethanol dispersion liquid dries naturally; the solid electrode is immersed into a high polymer film material solution after being dry; a solvent on the surface of the solid electrode is volatilized in a refrigerator, and then a layer of a complex enzyme function film comprising the methylene blue intercalation manganese dioxide can be formed on the surface of the solid electrode. The enzyme function sensitive film in the invention is used in the electrochemistry biological sensor and greatly improves the performances of the sensor such as detecting potential, anti-jamming capacity and stability.

Description

Technical field: [0001] The invention belongs to the technical field of electrochemical biosensor and its preparation, in particular to an electrochemical biosensor enzyme function sensitive membrane containing methylene blue intercalated manganese dioxide supramolecular structure material and a preparation method thereof. Background technique: [0002] Electrochemical biosensor is a rapidly developing cutting-edge analytical technology, which is widely used in chemistry, physics, medical treatment, materials, environmental protection, food and industrial processes due to its compact structure, low cost, sensitivity, and rapid in-situ analysis and monitoring. Automatic control and other fields. [0003] The sensor that uses the natural electron carrier of the enzyme—oxygen to communicate the electronic channel between the enzyme and the electrode, and directly detects the reduction of the substrate of the enzyme reaction or the generation of the product, is called the first ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327C12Q1/00
Inventor 杨文胜杨秀双张熊段雪
Owner BEIJING UNIV OF CHEM TECH
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