Complex enzyme function sensitive membrane containing hydrophili nano platinum particle and hydrophobic silicon dioxide particle, its manufacturing method and use

A nano-silica, silica technology, applied in the field of enzyme bioengineering, can solve the problem that the current response sensitivity of the enzyme electrode is not helpful

Inactive Publication Date: 2003-07-02
TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

American A.L.Crumbliss. et al. have introduced hydrophilic gold particles into biosensors and proved the biocompatibility of gold particles, but they used 50nm hydrophilic gold particles, which have no sensitivity to the current response of enzyme electrodes. Help, this article in "Biotechnology and Bioengineering" magazine, 1992, volume 40, pages 483-490 "is suitable for a biocompatible immobilization medium-colloidal gold as an electrodeposition method for assembling enzyme electrodes" It has been reported (A.L. Crumbliss, S.C. Perine, J. Stonehuemer, K.P. Tubergen, Junguo Zhao and R.W. Henkens, Biotechnology and Bioengineering, Vol.40 P.483-490 (1992))

Method used

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  • Complex enzyme function sensitive membrane containing hydrophili nano platinum particle and hydrophobic silicon dioxide particle, its manufacturing method and use
  • Complex enzyme function sensitive membrane containing hydrophili nano platinum particle and hydrophobic silicon dioxide particle, its manufacturing method and use
  • Complex enzyme function sensitive membrane containing hydrophili nano platinum particle and hydrophobic silicon dioxide particle, its manufacturing method and use

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Step 1: Prepare hydrophilic nano-platinum sol solution by sol-gel method:

[0073] The aqueous solutions of chloroplatinic acid, sodium borohydride and hydroxypropyl cellulose were prepared respectively, and the molar concentrations of chloroplatinic acid and sodium borohydride were 8×10 -4 mol / Kg and 6.3×10 -2 mol / Kg, the volume ratio of the solid content of hydroxypropyl cellulose to the solvent is 0.5 g / L. Mix the chloroplatinic acid aqueous solution and the hydroxypropyl cellulose aqueous solution at 55°C, the volume ratio is 1:0.5, and then add the mixed solution and the sodium borohydride aqueous solution to the reaction vessel respectively, wherein the chloroplatinic acid aqueous solution and the borohydrogenation The molar ratio of the sodium aqueous solution is 1: 15, and the mixed solution is reacted for 1 hour under stirring to obtain a particle diameter of 4 nm hydrophilic nano-platinum sol solution, and its molar concentration is 7 × 10 -4 mol / Kg.

[007...

Embodiment 2

[0084] Step 1: Prepare hydrophilic nano-platinum sol solution by sol-gel method:

[0085] The aqueous solutions of chloroplatinic acid, sodium borohydride and carboxymethyl cellulose were prepared respectively, and the molar concentrations of chloroplatinic acid and sodium borohydride were 8×10 -4 mol / Kg and 2.1×10 -2 mol / Kg, the volume ratio of the weight of carboxymethyl cellulose to the solvent is 0.03 g / L. Mix the chloroplatinic acid aqueous solution and the carboxymethyl cellulose aqueous solution at 45°C, the volume ratio is 1:1, and then add the mixed solution and the sodium borohydride aqueous solution into the reaction vessel respectively, wherein the chloroplatinic acid aqueous solution and the sodium borohydride The molar ratio of the aqueous solution is 1:5, and the mixed solution is reacted for 1.5 hours under stirring to obtain a particle diameter of 8nm hydrophilic nano-platinum sol solution, and its weight molar concentration is 7×10 -4 mol / Kg.

[0086] The ...

Embodiment 3

[0096] Step 1: Prepare hydrophilic nano-platinum sol solution by sol-gel method:

[0097] The aqueous solutions of chloroplatinic acid, hydrazine hydrate and polyvinylpyrrolidone were prepared respectively, and the molar concentrations of chloroplatinic acid and hydrazine hydrate were 8×10 -4 mol / Kg and 2×10 -1 mol / Kg, the volume ratio of the weight of polyvinylpyrrolidone to the solvent is 6 grams / liter. Mix the chloroplatinic acid aqueous solution and the polyvinylpyrrolidone aqueous solution at 80°C, the volume ratio is 1:5, and then add the mixed solution and the hydrazine hydrate aqueous solution into the reaction vessel respectively, wherein the molar ratio of the chloroplatinic acid aqueous solution and the hydrazine hydrate aqueous solution is 1 : 50, the mixed solution was reacted under stirring for 0.5 hour to obtain a particle diameter of 2nm hydrophilic nano-platinum sol solution, and its weight molar concentration was 7×10 -4 mol / Kg.

[0098] The second step: p...

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Abstract

A composite enzyme-function sensitive film containing hydrophilic nanometre Pt particles and hydrophobic nanometre SiO2 particles is prepared through mixing hydrophilic nanometre Pt sol, hydrophobic nanometre SiO2 sol and aqueous solution of oxidase, then adding to a solution of high-molecular gel, stirring, adding glutaric dialdehyde for cross-linking, and coating on the surface of a carrier. Its advantages are high sensitivity and stability and low consumption of enzyme.

Description

technical field [0001] The invention belongs to the technical field of enzyme bioengineering, and in particular relates to a composite enzyme function-sensitive membrane containing hydrophilic nano-platinum particles and hydrophobic nano-silicon dioxide particles with high sensitivity and high stability, as well as its preparation method and application. Background technique [0002] Products manufactured by enzyme bioengineering are widely used in human body and industry. For example, glucose biosensors assembled by glucose oxidase can be used to measure human blood sugar, industrial wastewater, sugar in fermentation or beverages. Biosensors have very obvious advantages, such as: high specificity, short-term low-cost analysis, no special requirements for analytical substances, safe operation, convenient on-site determination, etc., and many achievements have been made in many countries. Sensor research workers all know that the development of enzyme b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/40C12N11/00C12Q1/26G01N27/327
Inventor 唐芳琼任湘菱孟宪伟
Owner TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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