Complex enzyme function sensitive membrane containing hydrophili nano platinum particle and hydrophobic silicon dioxide particle, its manufacturing method and use

A nano-silica, silica technology, applied in the field of enzyme bioengineering, can solve the problem that the current response sensitivity of the enzyme electrode is not helpful
CN1427074AInactive Publication Date: 2003-07-02TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
Publication Date
2003-07-02
Estimated Expiration
Not applicable · inactive patent

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Abstract

A composite enzyme-function sensitive film containing hydrophilic nanometre Pt particles and hydrophobic nanometre SiO2 particles is prepared through mixing hydrophilic nanometre Pt sol, hydrophobic nanometre SiO2 sol and aqueous solution of oxidase, then adding to a solution of high-molecular gel, stirring, adding glutaric dialdehyde for cross-linking, and coating on the surface of a carrier. Its advantages are high sensitivity and stability and low consumption of enzyme.
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Description

technical field

[0001] The invention belongs to the technical field of enzyme bioengineering, and in particular relates to a composite enzyme function-sensitive membrane containing hydrophilic nano-platinum particles and hydrophobic nano-silicon dioxide particles with high sensitivity and high stability, as well as its preparation method and application. Background technique

[0002] Products manufactured by enzyme bioengineering are widely used in human body and industry. For example, glucose biosensors assembled by glucose oxidase can be used to measure human blood sugar, industrial wastewater, sugar in fermentation or beverages. Biosensors have very obvious advantages, such as: high specificity, short-term low-cost analysis, no special requirements for analytical substances, safe operation, convenient on-site determination, etc., and many achievements have been made in many countries. Sensor research workers all know that the development of enzyme b...

Claims

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