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Application of cubic boron nitride as mimetic peroxidase

A technology of boron nitride peroxide and cubic boron nitride, which is applied in the field of chemical detection of biological materials, can solve the problems of non-regeneration, poor tolerance, low catalytic efficiency, etc., and achieves low cost, simple preparation process and high stability. Effect

Active Publication Date: 2016-09-07
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These nanomaterials mimic enzymes generally have the disadvantages of low catalytic efficiency, poor tolerance and non-renewability.

Method used

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  • Application of cubic boron nitride as mimetic peroxidase
  • Application of cubic boron nitride as mimetic peroxidase
  • Application of cubic boron nitride as mimetic peroxidase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Weigh 50 mg of cubic boron nitride powder and place it in a centrifuge tube, add 10 ml of ultrapure water and ultrasonically disperse for 10 minutes to prepare a 5 mg / ml cubic boron nitride suspension. Take 3.7ml of citric acid-disodium hydrogen phosphate buffer solution with a pH of 4 and place it in a constant temperature water bath at 45°C for 10 minutes, then add 0.1ml of 3,3',5,5'-tetramethyl Benzidine (TMB) solution, 0.1ml of 10mmol / L hydrogen peroxide solution, and 0.1ml of cubic boron nitride solution, shake well, react at 45°C for 10 minutes, take out, and obtain the sample to be tested after filtration. Using ultrapure water as a blank, measure the absorption spectrum with a UV-Vis spectrophotometer in the wavelength range of 400-800nm.

[0043] In addition, in this implementation, X-ray diffraction, scanning electron microscopy and photoelectron spectroscopy analysis and characterization were performed on the above cubic boron nitride.

[0044] The X-ray dif...

Embodiment 2

[0048] Weigh 50 mg of cubic boron nitride powder and place it in a centrifuge tube, add 10 ml of ultrapure water and ultrasonically disperse for 10 minutes to prepare a 5 mg / ml cubic boron nitride suspension. Take 3.7ml of citric acid-sodium hydrogen phosphate buffer solution with pH of 2, 3, 4, 5, 6, 7 and 8 and sodium carbonate-sodium bicarbonate buffer solution with pH of 9, 10 and 11 respectively and place at constant temperature Incubate in a water bath at 45°C for 10 minutes, then add 0.1ml of 1mmol / L 3,3',5,5'-tetramethylbenzidine (TMB) solution and 0.1ml of 50mmol / L hydrogen peroxide solution in sequence , and 0.1ml of cubic boron nitride solution, shake well, react at 45°C for 10 minutes, take out, and obtain the sample to be tested after filtering. Using ultrapure water as a blank, the absorbance at the maximum absorption wavelength was measured with a UV-Vis spectrophotometer.

[0049] The relative peroxidase activity of cubic boron nitride peroxide mimetic enzymes a...

Embodiment 3

[0051] Weigh 50 mg of cubic boron nitride powder and place it in a centrifuge tube, add 10 ml of ultrapure water and ultrasonically disperse for 10 minutes to prepare a 5 mg / ml cubic boron nitride suspension. Take 3.7ml of citric acid-disodium hydrogen phosphate buffer solution with a pH of 4 and place it in a constant temperature water bath for 10 minutes. 3,3',5,5'-tetramethylbenzidine (TMB) solution with a concentration of 1mmol / L, 0.1ml of a 50mmol / L hydrogen peroxide solution, and 0.1ml of a cubic boron nitride solution, shake well , after reacting for 10 minutes at the corresponding temperature, take it out, and obtain the sample to be tested after filtering. Using ultrapure water as a blank, the absorbance at the maximum absorption wavelength was measured with a UV-Vis spectrophotometer.

[0052] The relative peroxidase activity of cubic boron nitride peroxide mimetic enzymes at different temperatures Figure 4b shown.

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Abstract

The invention discloses an application of cubic boron nitride as mimetic peroxidase. Specifically, cubic boron nitride is taken as mimetic peroxidase to measure the hydrogen peroxide concentration. Cubic boron nitride simulates peroxidase, the particle size of cubic boron nitride is not limited, and cubic boron nitride particles all have mimetic peroxidase activity. At first, people find that cubic boron nitride can simulate peroxidase; the enzyme catalytic activity of cubic boron nitride is as good as that of horse radish peroxidase (HRP); and cubic boron nitride can be used to measure the content of hydrogen peroxide in a solution sample. Compared with natural enzymes, cubic boron nitride (simulated enzyme) is more stable in extreme environments such as strong acid, strong alkali, high temperature, and the like, moreover, cubic boron nitride can be repeatedly used, and the catalytic activity is maintained in a high level. The preparation technology is simple, the repeatability and stability are high, and the cost is low. Cubic boron nitride is used as a novel mimetic peroxidase, and can replace peroxidase in fields such as immunoassay, biological detection, clinical diagnosis, pollutant degradation, and the like.

Description

technical field [0001] The invention relates to the field of chemical detection of biological materials, in particular to the application of cubic boron nitride as a peroxide mimetic enzyme. Background technique [0002] Natural enzymes have important applications in the fields of biology, chemistry, agriculture and food processing because of their high selectivity and high efficiency of catalytic activity. However, natural enzymes are susceptible to denaturation and inactivation due to experimental conditions such as temperature and pH, resulting in reduced stability, high prices, difficult purification, high storage and use costs, and these shortcomings greatly limit the scope of application of enzymes. Therefore, it is necessary to develop mimetic enzymes with catalytic properties of enzymes and more stable structures. [0003] Compared with natural enzymes, nanomaterials have the advantages of low cost, adjustable catalytic activity, high stability, and easy handling an...

Claims

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Application Information

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IPC IPC(8): G01N21/31
CPCG01N21/314G01N2021/3155
Inventor 杨国伟陈统铭肖俊刘璞
Owner SUN YAT SEN UNIV
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