The invention discloses a chemiluminiscence immuno biosensor detection device and a detection analysis method. The device comprises a sound energy transmission and chemiluminiscence immunoreaction detection device part and a control detection part, wherein the sound energy transmission and chemiluminiscence immunoreaction detection device part comprises an ultrasonic transducer, an ultrasonic coupling colloid, an acoustic focusing lens array integrated glass substrate, a replaceable chemiluminiscence immuno biosensing substrate, condensing transparent glass, a diaphragm, an optical lens and an optical signal detection circuit; the control detection part comprises an ultrasonic sensor, a proportion integration differentiation (PID) ultrasonic energy mode control module, a weak optical signal processing module, a data analysis and feedback control module, an energy control mode and chemiluminiscence immunoreaction system database. The method comprises the following steps: regulating a radiation energy field of the ultrasonic transducer; performing micro-flow injection analysis, and intelligently measuring and controlling ultrasonic transmitting energy. According to the device and method, the reaction efficiency is improved, and the detection sensitivity, accuracy, repeatability and stability are expanded.