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Chemiluminiscence immuno biosensor detection device and detection analysis method

A chemiluminescence immune and biosensor technology, applied in the field of biosensors, can solve problems such as background noise, interference, detection limit, sensitivity and repeat consistency reduction, etc., and achieve the effect of improving detection efficiency, low cost, and speeding up the actual reaction process.

Inactive Publication Date: 2014-02-26
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Even if the antibody / antigen that has crossed this energy barrier collides or binds within the effective reaction scale, there is still a certain probability of non-specific binding. This phenomenon will bring background noise and interference, resulting in detection limits, sensitivity and repeatability. Reduced performance such as consistency

Method used

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  • Chemiluminiscence immuno biosensor detection device and detection analysis method
  • Chemiluminiscence immuno biosensor detection device and detection analysis method

Examples

Experimental program
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Effect test

Embodiment 1

[0050] Example 1. As figure 1 , figure 2 As shown, the device of the present invention includes an acoustic energy transmission and chemiluminescent immunoreaction detection device part, and a control detection part.

[0051] The acoustic energy transmission and chemiluminescence immunoreaction detection device includes, from bottom to top, a fixed transducer substrate 111, an acoustic energy damping attenuation sheet 110, an ultrasonic transducer 101, an ultrasonic coupling colloid 102, and an integrated acoustic focusing lens array. Glass substrate 103 , replaceable chemiluminescent immunobiological sensing substrate 104 , silicon rubber sealing ring 109 , light-condensing transparent glass 107 , aperture and optical lens 108 , and optical signal detection circuit 106 .

[0052] The integrated glass substrate 103 of the acoustic focusing lens array, the silicone rubber sealing ring 109, and the light-condensing transparent glass 107 are sequentially arranged from bottom to...

Embodiment 2

[0065] Example 2. An analytical method for chemiluminescent immunobiological reaction assay using the above-mentioned device.

[0066] Step (1). Adjust the radiation energy field of the ultrasonic transducer 101

[0067] The focal length is adjusted by the size of the concave spherical surface of the acoustic lens on the ultrasonic coupling colloid 102, so that the vertical distance from the focal point to the surface of the replaceable chemiluminescence immunobiological sensing substrate 104 is 1-2 mm.

[0068] The characteristic size of the concave spherical surface of the acoustic lens on the ultrasonic coupling colloid 102 is determined by the characteristics of the ultrasonic frequency, the focused energy, the material and thickness of the polymer gold film electrode substrate 107, and the flow reaction chamber 105 of the flow injection reaction pool / injection device. Size and other specific parameters to determine.

[0069] The overall principle is: ① adjust the int...

Embodiment 3

[0081] Example 3. Preparation of replaceable chemiluminescent immunobiological sensing substrate 104

[0082] The preparation of immunosensors can be divided into two ways: online preparation and offline preparation. The present invention adopts off-line preparation, and the on-line preparation method is the content of another patent.

[0083] (1) Dissolve the antigen to be tested in a buffer solution. The selected buffer solution varies with the type of antigen. The standard is to maximize the activity of the immune reaction and the chemiluminescent signal response. How to judge can be judged by comparing the standard channels.

[0084] (2) Pretreat the surface of the slide carrier to obtain a flat, clean and hydrophilic surface.

[0085] (3) Prepare a certain concentration of epoxypropylene trimethylsilane solution, let it stand for 60 minutes to fully hydrolyze, then pipette 50 microliters of the solution and drop it on the surface of the carrier, and heat it at 93...

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Abstract

The invention discloses a chemiluminiscence immuno biosensor detection device and a detection analysis method. The device comprises a sound energy transmission and chemiluminiscence immunoreaction detection device part and a control detection part, wherein the sound energy transmission and chemiluminiscence immunoreaction detection device part comprises an ultrasonic transducer, an ultrasonic coupling colloid, an acoustic focusing lens array integrated glass substrate, a replaceable chemiluminiscence immuno biosensing substrate, condensing transparent glass, a diaphragm, an optical lens and an optical signal detection circuit; the control detection part comprises an ultrasonic sensor, a proportion integration differentiation (PID) ultrasonic energy mode control module, a weak optical signal processing module, a data analysis and feedback control module, an energy control mode and chemiluminiscence immunoreaction system database. The method comprises the following steps: regulating a radiation energy field of the ultrasonic transducer; performing micro-flow injection analysis, and intelligently measuring and controlling ultrasonic transmitting energy. According to the device and method, the reaction efficiency is improved, and the detection sensitivity, accuracy, repeatability and stability are expanded.

Description

technical field [0001] The invention belongs to the technical field of biosensors, and relates to a chemiluminescence immune biosensor detection device and a detection and analysis method, in particular to a chemiluminescence immune biosensor detection device and a chemiluminescence immunoassay assay method based on microfluidic injection based on the principle of ultrasonic chemistry . Background technique [0002] As an immunoanalysis method, immunobiosensors are widely used in various fields such as medical health, food safety, and environmental monitoring due to their good selectivity, fast analysis speed, easy operation, and high performance and price ratio of testing instruments. . In this context, how to develop biosensors with excellent performance, small variance, and high consistency has always been a key topic in the research and development field. In recent decades, immunoassay technology has combined the specific recognition reaction between antibody-antigen a...

Claims

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Application Information

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IPC IPC(8): G01N33/544G01N21/76
CPCG01N21/763G01N33/54366
Inventor 刘军邹倩谢斐周雅琪
Owner ZHEJIANG UNIV
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