Detection device for electrochemical immune biosensor and detection and analysis method
A biosensor and detection device technology, applied in the field of biosensors, can solve problems such as detection limit, sensitivity and repeat consistency reduction, interference, background noise, etc., and achieve the effect of speeding up and actual reaction process, improving efficiency, and improving detection efficiency.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0052] Example 1. As figure 1 , figure 2 , image 3 As shown, the device of the present invention includes an acoustic energy transmission and electrochemical immune reaction detection device part, and a control detection part.
[0053] The part of the electrochemical immune reaction detection device includes, from bottom to top, a transducer fixed substrate 110, an acoustic energy damping attenuation sheet 109, an ultrasonic transducer 101, an ultrasonic coupling colloid 102, an acoustic focusing lens array integrated glass substrate 103, and synaptic contacts. Silicone rubber sealing ring 108, replaceable electrochemical immunobiological sensitive film substrate 104, polymer gold film electrode substrate 107, multi-channel weak electric signal detection circuit 106;
[0054] The integrated glass substrate 103 of the acoustic focusing lens array, the contact silicon rubber sealing ring 108, and the polymer gold film electrode substrate 107 are sequentially arranged from bo...
Embodiment 2
[0067] Embodiment 2. An analysis method for electrochemical immunobiological reaction measurement, specifically a microfluidic injection electrochemical immunobiological detection analysis method that utilizes controllable ultrasonic radiation energy to improve the efficiency of electrochemical immune reaction.
[0068] Step (1). Adjust the radiation energy field of the ultrasonic transducer 101
[0069] The focal length is adjusted by the size of the concave spherical surface of the acoustic lens on the ultrasonic coupling colloid 102, so that the vertical distance from the focal point to the surface of the replaceable electrochemical immunobiological sensitive membrane substrate 104 is 1-2 mm.
[0070] The characteristic size of the concave spherical surface of the acoustic lens on the ultrasonic coupling colloid 102 is determined by the characteristics of the ultrasonic frequency, the focused energy, the material and thickness of the polymer gold film electrode substrate 107...
Embodiment 3
[0083] Example 3. The typical preparation process of the replaceable electrochemical immunobiologically sensitive membrane substrate 104: ① Dissolving the antigen to be tested in a buffer solution, the selected buffer solution varies with the type of antigen, the principle is to make the activity and chemical reaction of the immune reaction Luminescent signal response is maximized. ② Pre-treat the surface of the sensor carrier to obtain a flat, clean and hydrophilic surface. ③ Prepare a certain concentration of epoxypropylene trimethylsilane solution, let it stand for 60 minutes to fully hydrolyze, then pipette 50 microliters of the solution and drop it on the surface of the carrier, and heat it at about 90°C for 60 minutes. Drop 50 microliters of 1% chitosan-acetic acid solution on the glass slide treated with silanization of GPS, and then place it in an oven and heat it at 105°C for 60 minutes to obtain a silane-crosslinked glutaraldehyde-based film. ④ Drop 50 microliters o...
PUM
Property | Measurement | Unit |
---|---|---|
Height | aaaaa | aaaaa |
Thickness | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com