In-situ biological repairing method for biomass intensified petroleum contaminative soil
A bioremediation and oil pollution technology, applied in the field of environmental remediation, can solve the problems of destroying soil aggregate structure, complex process, secondary pollution, etc., and achieve the effect of promoting bioaccumulation, promoting uptake, and overcoming ecological reasons
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[0028] In the example, the oil-contaminated soil contains 3.75% oil. The implementation process is as follows:
[0029] 1) First, mix the wheat straw with the pre-cultured Phanerochaete chrysosporium and Pseudomonas aeruginosa. The addition amount of Phanerochaete chrysosporium is 50mg / Kg wheat straw, and the amount of Pseudomonas aeruginosa is 10 10 CFU / Kg wheat straw;
[0030] 2) The oil-contaminated soil is contained in a pot with a depth of about 25cm. Mix the wheat straw mixed with Phanerochaete chrysosporium and petroleum-contaminated soil thoroughly. The addition amount of wheat straw is 5% of the soil volume; keep the water content 15-25% for 30 days;
[0031] 3) Applying a petroleum hydrocarbon degrading compound bacterial agent composed of Enterobacter cloacae, Pseudomonas aeruginosa and Cunninghamella chinensis, wherein Pseudomonas aeruginosa is a high-efficiency producer of surfactants. The amount of bacteria added is 10 10 CFU / m 2 Soil, fungus is 50mg dry weight / m 2 s...
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