Super-high-temperature-resistant and alkali-resistant beta-mannase gene, amino acid sequence and application of beta-mannase gene

A mannanase and base sequence technology, applied in the application of ultra-high temperature and alkali resistance β-mannanase gene, ultra-high temperature and alkali resistance β-mannanase gene, ultra-high temperature and alkali resistance β-mannanase gene Amino acid sequence domain of mannanase

Inactive Publication Date: 2015-06-24
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
View PDF1 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research on β-mannanase in my country has been started since the 1980s, and related enzymes have also been reported, but they belong to the thermophilic β-mannanase of th

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Super-high-temperature-resistant and alkali-resistant beta-mannase gene, amino acid sequence and application of beta-mannase gene
  • Super-high-temperature-resistant and alkali-resistant beta-mannase gene, amino acid sequence and application of beta-mannase gene
  • Super-high-temperature-resistant and alkali-resistant beta-mannase gene, amino acid sequence and application of beta-mannase gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Genetic improvement and breeding of ultra-high temperature and alkali-resistant β-mannanase and the purification and application of ultra-high temperature and alkali-resistant β-mannanase

[0044] The present invention also provides an application of an ultra-high temperature and alkali-resistant β-mannanase gene in the preparation of an ultra-high temperature and alkali-resistant β-mannanase, comprising the following steps:

[0045] (1) Bacillus sp. strain HFS06, which simultaneously degrades cellulose, xylan and mannan, was isolated from tropical forestry soil samples. After 16S rRNA gene sequence determination, molecular identification shows that it belongs to Bacillus subtilis subsp.Spizizenii subspecies, and the sequence is shown in SEQ ID No.3. Select bacterial strain HFS06 to make the suspension of concentration about 10 ^ 6cfu / ml, use chemical mutagen ethyl methanesulfonate (EMS) to mutate under different treatment concentrations and different action t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a super-high-temperature-resistant and alkali-resistant beta-mannase gene. A base sequence of the beta-mannase gene is SEQ ID NO.1. The invention further discloses a nucleotide sequence SEQ ID NO.2 of super-high-temperature-resistant and alkali-resistant beta-mannase, and further discloses application of the super-high-temperature-resistant and alkali-resistant beta-mannase gene in preparation of the super-high-temperature-resistant and alkali-resistant beta-mannase. The obtained expression enzyme can widely endure alkaline (pH7-10) and high-temperature (80-100 DEG C) environments; and the specific activity of the purified expression enzyme can be up to 10400U/mg. The beta-mannase has super-high-temperature-resistant and alkali-resistant characteristics, and is suitable for application in the chemical industries such as biomass degradation, foods, feeds, papermaking and spinning.

Description

technical field [0001] The invention belongs to the technical field of microbial enzyme engineering, and specifically relates to an ultra-high temperature and alkali-resistant β-mannanase gene. The invention also discloses an amino acid sequence of an ultra-high temperature and alkali-resistant β-mannanase. Also disclosed is the application of an ultrahigh temperature and alkali resistant β-mannanase gene. Background technique [0002] Mannan is the main component of hemicellulose in biomass, and β-mannanase plays an important role in the development and utilization of natural hemicellulose resources and the research and development of mannan-oligosaccharide drugs. β-mannanase (β-1,4-mannan mannohydrolase, EC 3.2.1.78), also known as β-D-mannanase or β-1,4-D-mannanase, is a class of Endohydrolase capable of hydrolyzing mannan oligosaccharides and mannan polysaccharides (including mannan, galactomannan, glucomannan, etc.) containing β-1,4-D-mannosidic bonds. It belongs to t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/56C12N9/42C12N15/70C12N15/01C12N13/00C12R1/125
Inventor 胡传炯万霞龚阳敏江木兰石燕黄凤洪
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap