Method for preparing pure biomass organophosphorus pesticide degradation agent
A technology of organophosphorus pesticides and degradation agents, which is applied in the direction of protection devices against harmful chemicals, etc., can solve the problems of slow degradation of degrading bacteria, loss of degradation ability, accelerated degradation of pesticides, etc., to achieve complete degradation, fast degradation, no The effect of secondary pollution
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example 1
[0023] 13. First weigh 200g of surface sludge from a pesticide production plant, 200g of activated sludge from a sewage treatment plant, and 300g of ordinary surface soil, mix the three types of soil evenly, take 4g of the mixed soil, and add it to 100mL of inorganic salt medium Medium; Add 40mL of organophosphorus pesticide with a concentration of 50mg / L to the above inorganic salt medium, place it in an incubator, and cultivate it statically at 25°C, and re-inoculate the mixed soil at 15% inoculum every 5 days. Inoculate in fresh inorganic salt medium, increase the content of organophosphorus pesticides at a rate of 50mg / L each time the inoculation is transferred, until the concentration reaches 400mg / L; keep the concentration of organophosphorus pesticides at 400mg / L, repeat the steps for transfer culture Acclimate for 1 month. After acclimatization, select the colony with the fastest growth and the largest area on the surface of inorganic salts, pick the colony with an inoc...
example 2
[0028] 17. First weigh 250g of surface sludge from a pesticide production plant, 250g of activated sludge from a sewage treatment plant, and 350g of ordinary surface soil, mix the three types of soil evenly, take 4.5g of the mixed soil, and add it to 100mL of inorganic salt culture Add 45mL of organophosphorus pesticide with a concentration of 75mg / L to the above-mentioned inorganic salt medium, place it in an incubator, and culture it at 30°C, and inoculate the mixed soil at 15% of the inoculum every 6 days. Re-inoculate in fresh inorganic salt medium, increase the content of organophosphorus pesticides at a rate of 75mg / L each time the inoculation is transferred, until the concentration reaches 400mg / L; keep the concentration of organophosphorus pesticides at 400mg / L, repeat the steps to transfer Cultivate and acclimatize for 1.5 months. After acclimatization, select the colony with the fastest growth and the largest area on the surface of inorganic salts, pick the colony wit...
example 3
[0033] 21. First weigh 300g of surface sludge from a pesticide production plant, 300g of activated sludge from a sewage treatment plant, and 400g of ordinary surface soil, mix the three types of soil evenly, take 5g of the mixed soil, and add it to 100mL of inorganic salt medium Medium; Measure 50mL of organophosphorus pesticide with a concentration of 100mg / L and add it to the above inorganic salt medium, place it in an incubator, and culture it statically at 35°C, and re-inoculate the mixed soil every 7 days according to the inoculum size of 15%. Inoculate in fresh inorganic salt medium, increase the content of organophosphorus pesticides at a rate of 100mg / L each time the inoculation is transferred, until the concentration reaches 400mg / L; keep the concentration of organophosphorus pesticides at 400mg / L, repeat the steps for transfer culture Acclimate for 2 months. After acclimatization, select the colony with the fastest growth and the largest area on the surface of inorgan...
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