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Method for preparing pure biomass organophosphorus pesticide degradation agent

A technology of organophosphorus pesticides and degradation agents, which is applied in the direction of protection devices against harmful chemicals, etc., can solve the problems of slow degradation of degrading bacteria, loss of degradation ability, accelerated degradation of pesticides, etc., to achieve complete degradation, fast degradation, no The effect of secondary pollution

Inactive Publication Date: 2016-06-01
袁春华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The main technical problem to be solved by the present invention is that the degradation speed of biomass pesticide-degrading bacteria directly screened from the environment is slow, and the fluctuations of temperature, pH and humidity are also large, and frequent mutations lead to the loss of degradation ability and the inability to continue to degrade pesticides. Problem, provide a kind of preparation method of pure biomass organophosphorus pesticide degradation agent, the present invention cultivates, separates and screens out a large amount of organophosphorus pesticide degrading bacteria from the mixed soil of surface sludge, activated sludge and common surface soil, and bacteria The silk suspension is mixed with humus and chitosan and aged to obtain a pasty mixture. The pasty mixture is mixed with nano-titanium dioxide and garlic extract concentrate and granulated. High degradation activity, wide adaptability to temperature and pH, combined with humic acid and other substances, it can degrade organophosphorus pesticides more thoroughly and quickly, among which allicin and alliin in garlic extract concentrate Pesticides have a degradation effect, and the photocatalytic properties of nano-titanium dioxide can also accelerate the degradation of pesticides, and the prepared pure biomass degradation agent is non-toxic, has no residue, and has no secondary pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0023] 13. First weigh 200g of surface sludge from a pesticide production plant, 200g of activated sludge from a sewage treatment plant, and 300g of ordinary surface soil, mix the three types of soil evenly, take 4g of the mixed soil, and add it to 100mL of inorganic salt medium Medium; Add 40mL of organophosphorus pesticide with a concentration of 50mg / L to the above inorganic salt medium, place it in an incubator, and cultivate it statically at 25°C, and re-inoculate the mixed soil at 15% inoculum every 5 days. Inoculate in fresh inorganic salt medium, increase the content of organophosphorus pesticides at a rate of 50mg / L each time the inoculation is transferred, until the concentration reaches 400mg / L; keep the concentration of organophosphorus pesticides at 400mg / L, repeat the steps for transfer culture Acclimate for 1 month. After acclimatization, select the colony with the fastest growth and the largest area on the surface of inorganic salts, pick the colony with an inoc...

example 2

[0028] 17. First weigh 250g of surface sludge from a pesticide production plant, 250g of activated sludge from a sewage treatment plant, and 350g of ordinary surface soil, mix the three types of soil evenly, take 4.5g of the mixed soil, and add it to 100mL of inorganic salt culture Add 45mL of organophosphorus pesticide with a concentration of 75mg / L to the above-mentioned inorganic salt medium, place it in an incubator, and culture it at 30°C, and inoculate the mixed soil at 15% of the inoculum every 6 days. Re-inoculate in fresh inorganic salt medium, increase the content of organophosphorus pesticides at a rate of 75mg / L each time the inoculation is transferred, until the concentration reaches 400mg / L; keep the concentration of organophosphorus pesticides at 400mg / L, repeat the steps to transfer Cultivate and acclimatize for 1.5 months. After acclimatization, select the colony with the fastest growth and the largest area on the surface of inorganic salts, pick the colony wit...

example 3

[0033] 21. First weigh 300g of surface sludge from a pesticide production plant, 300g of activated sludge from a sewage treatment plant, and 400g of ordinary surface soil, mix the three types of soil evenly, take 5g of the mixed soil, and add it to 100mL of inorganic salt medium Medium; Measure 50mL of organophosphorus pesticide with a concentration of 100mg / L and add it to the above inorganic salt medium, place it in an incubator, and culture it statically at 35°C, and re-inoculate the mixed soil every 7 days according to the inoculum size of 15%. Inoculate in fresh inorganic salt medium, increase the content of organophosphorus pesticides at a rate of 100mg / L each time the inoculation is transferred, until the concentration reaches 400mg / L; keep the concentration of organophosphorus pesticides at 400mg / L, repeat the steps for transfer culture Acclimate for 2 months. After acclimatization, select the colony with the fastest growth and the largest area on the surface of inorgan...

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PUM

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Abstract

The invention discloses a method for preparing a pure biomass organophosphorus pesticide degradation agent and belongs to the field of pesticide degradation agents. According to the method, a large number of organophosphorus pesticide degrading bacteria are cultured, separated and screened out from mixed soil of earth surface sludge, activated sludge and common earth surface soil; hypha suspension liquid, humus and chitosan are mixed and aged to obtain a pasty mixture; and the pasty mixture, nano titanium dioxide and a garlic extract concentrated solution are mixed and pelletized. The organophosphorus pesticide degrading bacteria screened and acclimatized through the method have high degradation activity, and the temperature and pH applicable range is wide; the pure biomass organophosphorus pesticide degradation agent is combined with substances such as humic acid and can degrade organophosphorus pesticide more thoroughly and faster; allicin and alliin in the garlic extract concentrated solution have a degradation effect on the organophosphorus pesticide; degradation of the pesticide can be accelerated through photocatalysis of the nano titanium dioxide; moreover, the prepared pure biomass degradation agent is non-toxic, has no residues and is free of secondary pollution.

Description

technical field [0001] The invention discloses a preparation method of a pure biomass organophosphorus pesticide degradation agent, belonging to the field of pesticide degradation agents. Background technique [0002] A large number of studies have shown that the continuous use of a certain pesticide will cause the degradation rate of this pesticide in the soil to accelerate. It has been confirmed that the cause of this phenomenon is mainly caused by microorganisms that can degrade pesticides, and the ability of these microorganisms to degrade pesticides is partly inherent and partly induced by the use of pesticides. Soil microorganisms include bacteria, fungi, actinomycetes, algae, etc., which can degrade pesticides. Bacteria play a major role in the microbial degradation of pesticides because of their biochemical adaptability and the ability to easily induce mutations. Pesticide-degrading bacteria in soil are not special microorganisms, they are widely distributed in soi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A62D3/02A62D3/17A62D3/30A62D101/26A62D101/04
CPCA62D3/02A62D3/17A62D3/30A62D2101/04A62D2101/26A62D2203/02
Inventor 袁春华王志慧林大伟
Owner 袁春华
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