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43 results about "Methylobacterium organophilum" patented technology

Methylobacterium organophilum is a facultatively methylotrophic bacteria from the genus of Methylobacterium which was isolated from sediments from the Lake Mendota in Madison in the United States. Methylobacterium organophilum can degrade methanol.

Salt-tolerant microbial agent and preparation method thereof

The invention discloses a salt-tolerant microbial agent and a preparation method thereof. The microbial agent contains staphylococcus cohnii FSND-C, arthrobacter creatinolyticus FDN-1, flavobacterium mizutaii FDN-2, paracoccus denitrificans DN-3 and methylobacterium phyllosphaerae SDN-3. The microbial agent can achieve removal of ammonia nitrogen, total nitrogen and CODcr in the same reactor, has a good wastewater treatment effect, and can achieve short-cut nitrification and denitrification or simultaneous nitrification and denitrification while removing COD. Staphylococcus cohnii FSND-C can utilize various carbon sources, has certain salt tolerance, can be applied to the high-salinity wastewater treatment process, simultaneously can secrete a substance under environmental stimulus to enhance the flocculability of sludge, and further widens the application range of the microbial agent.
Owner:CHINA PETROLEUM & CHEM CORP +1

Biological denitrification method for salt-containing sewage

The invention relates to a biological denitrification method for salt-containing sewage, which comprises the following steps: adding a denitrification microbial inoculant into a biochemical sewage treatment system, and simultaneously starting the nitrification-denitrification biological denitrification treatment processes, wherein the sewage treatment temperature is 18-40 DEG C, the dissolved oxygen is 0.1-5 mg / L, and the pH value is 7.0-9.0. The denitrification microbial inoculant contains one or two of Staphylococcuscohnii FSDN-C, Arthrobactercreatinolyticus FDN-1 and Flavobacteriummizutaii FDN-2, and also contains one or two of Paracoccusdenitrificans DN-3 and Methylobacteriumphyllosphaerae SDN-3. The method provided by the invention can effectively treat high-salt-content sewage, enhances the adsorptivity and flocculence for sludge, has wide application range for wastewater quality and high impact resistance to salt-containing sewage, and obviously enhances the sewage treatment effect on the premise of removing pollutants, such as ammonia nitrogen, COD (chemical oxygen demand) and the like.
Owner:CHINA PETROLEUM & CHEM CORP +1

Nitrous acid type biological denitrogenation microbial agent and application thereof

The present invention discloses a short-cut nitrification-denitrification microbial agent. The microbial agent contains Arthrobacter creatinolyticus FDN 1, Flavobacterium mizutaii FDN 2, Paracoccus denitrificars DN 3 and Methylobacterium phyllosphaerae SDN3. The preservation register numbers of the four strains respectively are CGMCC No.3657, CGMCC No.3659, CGMCC No.3658 and CGMCC No.3660. With the microbial agent, the ammonia nitrogen removing, the total nitrogen removing and the CODcr removing in the same reactor can be achieved, the wastewater treatment effect is good, and the microbial agent is especially suitable for the purification treatment of the wastewater containing the ammonia nitrogen so as to really achieve the short-cut nitrification-denitrification or simultaneous nitrification and denitrification.
Owner:CHINA PETROLEUM & CHEM CORP +1

Regulation of heterologous recombinant protein expression in methylotrophic and methanotrophic bacteria

Methylotrophic or methanotrophic bacteria such as Methylobacterium are transformed with a gene of interest, and expression of the gene is regulated by means of a cumate repressor protein and an operator sequence which is operatively linked to the gene of interest, and the addition of an external agent. Specifically, the cymR repressor and cmt operator from Pseudomonas putida may serve to regulate gene expression in methylotrophic or methanotrophic bacteria with the addition of cumate.
Owner:NAT RES COUNCIL OF CANADA

Methods and compositions for degradation of nitroaromatic and nitramine pollutants

The invention relates to novel Methylobacterium species that are capable of degrading nitroaromatic and nitramine compounds. Compositions, kits and methods of using the Methylobacterium species for the degradation of nitroaromatic and nitramine pollutants are provided. More specifically, compositions and methods for the degradation or bioremediation of nitroaromatic and nitramine explosives and explosive residues are provided.
Owner:UNIV OF IOWA RES FOUND

Degrading bacterium for TC (tetracycline) antibiotics and application of degrading bacterium

The invention relates to a degrading bacterium Methylobacterium sp. YWF1 capable of degrading three TC (tetracycline) antibiotics, namely, TC, OTC (oxytetracycline) and CTC (Chlorotetracycline) simultaneously. The degrading bacterium YWF1 is identified as Methylobacterium and was preserved in CGMCC (China General Microbiological Culture Collection Center) on April 17, 2017 with preservation number being CGMCCNo. 14040. Methylobacterium sp. YWF1 can simultaneously degrade the three TC antibiotics, namely, TC, OTC and CTC, a microbial agent suspension can be prepared from the bacterium to be applied to degrading removal of TC antibiotic pollutants in different environment media such as livestock wastewater, TC pharmaceutical enterprise wastewater, medical wastewater or soil, and has good industrial application prospect and environmental benefits.
Owner:众心通慧(厦门)生态环保科技有限公司

Composition for preventing odor, containing odorless microorganisms

Disclosed is a coating composition for preventing odor. The coating composition may include one or more types of odorless microorganisms selected from the group consisting of Caulobacter vibrioides, Bradyrhizobium diazoefficiens, Bradyrhizobium daqingense and Methylobacterium brachiatum or a culture solution thereof. Further provided is a method for preventing odor, and the method may include applying the coating composition for preventing odor. When a biofilm is formed from the microorganisms in the coating composition by coating an object on which an odor-causing microorganism can live, the inflow and the inhabitation of external microorganisms generating odor may be significantly blocked, thereby enabling odor to be effectively prevented.
Owner:HYUNDAI MOTOR CO LTD

Microbial agent for efficiently degrading formaldehyde and preparation method of microbial agent

The invention discloses a microbial agent for efficiently degrading formaldehyde and a preparation method of the microbial agent, belongs to the technical field of indoor air purification, and solvesthe technical problem that an existing single microbial agent cannot efficiently degrade low-concentration formaldehyde. The preparation method of the microbial agent comprises the following steps: S1, activating Methylobacterium, Pseudomonas and Rhodopseudomonas palustris for standby application; S2, inoculating the activated Methylobacterium and activated Pseudomonas into a liquid culture mediumA for culturing for 12-18 h, then inoculating the activated Rhodopseudomonas palustris into the liquid culture medium A, and performing culturing in a dark environment with formaldehyde; and S3, centrifuging the microbial culture solution, and performing cell disruption, membrane filtration and drying on thalli to obtain the microbial agent. The microbial agent disclosed by the invention is usedfor degrading indoor formaldehyde, and has the advantage of efficiently degrading indoor low-concentration formaldehyde.
Owner:北京首诚田园科技发展有限公司

Regulation of heterologous recombinant protein expression in methylotrophic and methanotrophic bacteria

Methylotrophic or methanotrophic bacteria such as Methylobacterium are transformed with a gene of interest, and expression of the gene is regulated by means of a cumate repressor protein and an operator sequence which is operatively linked to the gene of interest, and the addition of an external agent. Specifically, the cymR repressor and cmt operator from Pseudomonas putida may serve to regulate gene expression in methylotrophic or methanotrophic bacteria with the addition of cumate.
Owner:NAT RES COUNCIL OF CANADA

Bacterial fermentation methods and compositions

The present invention provides methods for the cultivation of the Methylobacterium genus of bacteria. In particular the method provides methods for the efficient and inexpensive cultivation of these bacteria. Additionally, the invention provides methods for the utilization of these bacterial cultures to improve plant agriculture.
Owner:NEWLEAF SYMBIOTICS INC

Salt-tolerant microbial agent and preparation method thereof

The invention discloses a salt-tolerant microbial bacterial agent and a preparation method thereof. The microbial bacterial agent contains Staphylococcus coli FSDN-C, Arthrobacter FDN-1, Flavobacterium waterii FDN-2, Paracoccus denitrogeni DN-3 and Methylobacterium sdn-3. The microbial bacterial agent can realize the removal of ammonia nitrogen, total nitrogen and CODcr in the same reactor, has good wastewater treatment effect, and can realize short-range nitrification and denitrification or simultaneous nitrification and denitrification denitrification while removing COD. Staphylococcus koshii FSDN-C can utilize a variety of carbon sources and has a certain salt tolerance, which can be used in the treatment of high-salt wastewater. At the same time, the strain can secrete a substance to enhance sludge flocculation under environmental stimulation sex. This further broadens the scope of application of the microbial agent.
Owner:CHINA PETROLEUM & CHEM CORP +1

Methylobacterium thermophilum and application thereof in organic solid wastes

The invention relates to the technical field of biology, in particular to methylobacterium thermophilum. The preservation number of the methylobacterium thermophilum is CGMCC No. 20326. The methylobacterium thermophilum can utilize heat generated by the fermentation of compost raw materials as a heat source for propagation and growth, the highest growth temperature is up to 77 DEG C or above, themethylobacterium thermophilum can quickly proliferate in a high-temperature composting environment, and organic matters are degraded to promote the composting process, do that the dual effects of heatenergy utilization and waste treatment are achieved; in addition, microbial inoculum prepared from bacterial strains has low water content, so that storage and transportation are facilitated; the microbial inoculum can be directly mixed with a stacked material during use, so that operation is convenient, and the water content of the material is not increased; and the microbial inoculum is a goodwet-based material conditioner, and has wide application value.
Owner:SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI

Isolated bacterial strain and application thereof

The invention discloses an isolated methylbacterium sp. DM1, which can decompose N, N-Dimethyl formamide. Methylbacterium sp. DM1 can use DMF as the sole carbon and nitrogen source to grow, mineralizeit thoroughly and release ammonia nitrogen. It can tolerate and completely degrade 6800 mg / L DMF in water. Under the condition of 30 DEG C and pH 7.0, 2000 mg / L DMF, methylbacterium sp. DM1 can be degraded completely within 28 hours, and the degradation rate reaches 71.4 mg / L / h, which is one of the fastest reported strains. Methylbacterium sp. DM1 has a good application prospect in environmentalsewage treatment and environmental remediation.
Owner:SHANGHAI JIAO TONG UNIV

Methylotrophs for aquaculture and animal feed

Disclosed are methods of producing carotenoid compounds in a methylotrophic bacterial host cell. Such a host cell may be an unmodified Methylobacterium, spontaneous mutant, or transformed cell, any of which exhibit favorable properties, such as overproduction of carotenoid compounds, increased carbon flux, improved growth, or the production of additional nutrients, such as protein, vitamins, antioxidants, or fatty acids. Also disclosed are feed compositions for use in aquaculture, or as animal feed, or as human nutritional supplements containing processed or unprocessed biomass from such cells, as are methods of preparation of the feed compositions.
Owner:KNIPBIO INC

Method for efficiently degrading indoor formaldehyde

The invention relates to the technical field of indoor air purification, and particularly discloses a method for efficiently degrading indoor formaldehyde. The method comprises the steps of S1, activating methyl bacillus, pseudomonas and rhodopseudomonas palustris for later use; S2, inoculating activated methylobacterium and activated pseudomonas into a liquid culture medium A, culturing for 12-18hours, inoculating activated rhodopseudomonas palustris into the liquid culture medium A, and culturing in a dark environment with formaldehyde; S3, co-culturing the microorganisms and green plants,and adding an amino acid solution into a culture solution to obtain potted plants capable of degrading indoor formaldehyde; and S4, adding the amino acid solution into the potted plants every other 2-20 days. The method disclosed by the invention has the advantage of efficiently degrading indoor low-concentration formaldehyde.
Owner:BEIJING SHOUCHENG AGRI DEV

Methylorubrum rhodesianum M520 and application thereof

The invention provides a strain of methylorubrum rhodesianum M520. The methylorubrum rhodesianum M520 is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number isGDMCC No.60729. The invention also provides an application of the M520 strain to preparation of a preparation for preventing and controlling root-knot nematodes or promotion of plant growth. When a metabolism crude extract of the methylorubrum rhodesianum M520 strain is applied to the root-knot nematodes, the lethal rate of the root-knot nematodes exceeds 96.6%; and in addition, a fermentation culture of the strain has an obvious promotion effect on growth of vegetable plants while effectively preventing and controlling the root-knot nematodes of potted vegetables, the use range and efficacy of methylobacterium are expanded, the prevention and control measures of a root-knot nematode disease is enriched, and the growth rate and vegetable yield of the vegetable plants are improved.
Owner:MOON (GUANGZHOU) BIOTECH CO LTD +1

A kind of microbial inoculum for efficiently degrading formaldehyde and preparation method thereof

The application discloses a microbial inoculant for efficiently degrading formaldehyde and a preparation method thereof, belonging to the technical field of indoor air purification, and solving the technical problem that the existing single microbial inoculant cannot efficiently degrade low-concentration formaldehyde. The preparation method of microbial inoculum comprises the following steps: S1, activate Methylobacterium, Pseudomonas and Rhodopseudomonas swamp, for standby use; S2, inoculate the activated Methylobacterium and the activated Pseudomonas into a liquid After culturing in medium A for 12-18 h, inoculate the activated Rhodopseudomonas swamp into liquid medium A, and cultivate in a dark environment with formaldehyde; S3, centrifuge the microbial culture solution, and remove the bacterial cells The microbial inoculum is obtained after the cells are broken, passed through the membrane and dried. The microbial inoculum of the present application is used for indoor formaldehyde degradation, and has the advantage of efficiently degrading indoor low-concentration formaldehyde.
Owner:北京首诚田园科技发展有限公司

Antifungal methylobacterium compositions and methods of use

Compositions comprising Methylobacterium with anti-fungal activity, methods for controlling plant pathogenic fungi, and methods of making the compositions are provided.
Owner:NEWLEAF SYMBIOTICS INC

Denitrification method of wastewater generated in process of producing catalytic cracking catalysts

The invention discloses a denitrification method of wastewater generated in the process of producing catalytic cracking catalysts. The method comprises the step of adding nitrobacteria and a denitrification agent to sewage, wherein the denitrification agent contains arthrobactercreatinolyticus FDN-1, flavobacteriummizutaii FDN-2, paracoccusdenitrificans DN-3 and methylobacteriumphyllosphaerae SDN-3. The method adopts autotrophic bacteria and heterotrophic bacteria which are subjected to combination of superiority to serve as reinforced microorganisms for wastewater treatment, adopts different addition modes, can achieve removal of ammonia nitrogen, total nitrogen and CODcr in the same reactor, has a good wastewater treatment effect, truly achieves short-cut nitrification and denitrification or simultaneous nitrification and denitrification of ammonia-containing catalyst wastewater by being combined with high-efficiency devices, and achieves up-to-standard discharge of pollutants such as ammonia nitrogen and the like in the wastewater.
Owner:CHINA PETROLEUM & CHEM CORP +1

A kind of butadiene producing bacteria and its method for producing butadiene

The invention relates to a butadiene producing strain taking methyl alcohol as a raw material and a method for producing butadiene, and belongs to the technical field of construction of engineering bacteria. The butadiene producing strain belongs to methylobacterium, the producing strain belongs to methylobacterium, and the producing strain is obtained by that foreign genes of the following enzymes are expressed by methylobacterium and then transferred into modification and expression, wherein the following enzymes include butadiene synthetase MTS or ISPS, crotyl alcohol mono-phosphate kinaseIPK, crotyl alcohol kinase THK or MK or TK an coenzyme A reductase FAR or BLD-BDH or ADHE2; and methylobacterium contains a serine circulation route and an acetyl malonyl-CoA route. The butadiene producing strain provided by the invention can take methyl alcohol as the raw material to produce butadiene, and can sufficiently utilize the raw material of methyl alcohol which has the competitive priceand is sufficiently supplied, and a butadiene product is directly synthesized through microbial metabolism. The cost is greatly reduced, and achievement of industrialized production of the procedurethat butadiene is synthesized by methyl alcohol is facilitated.
Owner:QINGDAO AGRI UNIV

A kind of Methylobacterium thermophilus and its application in organic solid waste

The invention relates to the field of biotechnology, in particular to a Methylobacter thermophilus. The present invention provides a Methylobacter thermophilus, the deposit number of which is CGMCC No.20326. The M. thermophilus provided by the invention can use the heat of self-fermentation of compost raw materials as a heat source to reproduce and grow, and the maximum growth temperature is as high as 77°C or more, can rapidly multiply in a high-temperature composting environment, degrade organic matter, promote the composting process, and achieve thermal energy. The dual effect of utilization and waste disposal. In addition, the inoculum prepared by this strain has a low moisture content, which is beneficial to storage and transportation. It can be directly mixed with the stacking material when used. It is easy to operate and will not increase the moisture content of the material. It is also a good wet-based material conditioner. , has a wide range of application value.
Owner:SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI

Methylobacterium sp. nov. strain, compositions comprising it, and its use as biostimulant and endophyte nitrogen-fixing bacterium

A Methylobacterium sp. nov. strain, deposited under accession number CECT 9580, to compositions comprising it, and its use as bio-stimulant and endophyte nitrogen-fixing bacterium in plants.
Owner:SYMBORG SL

Methylobacterium, fermentation product thereof and application of methylobacterium in inhibition of growth of algae

The invention discloses methylobacterium, a fermentation product thereof and application of the methylobacterium to inhibition of algae growth, and belongs to the technical field of microorganisms. The preservation number of the methylobacterium 12-2 is GDMCC No: 62327, and the methylobacterium 12-2 has a remarkable algal inhibition effect on apis clostridium, chlorella pyrenoidosa and microcystis aeruginosa, so that the methylobacterium 12-2 is rich in algal inhibition spectrum and has very strong application potential in the aspect of biological agents for controlling algal blooms.
Owner:GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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