Methylobacterium thermophilum and application thereof in organic solid wastes

A Methylobacterium and substrate technology, applied in the biological field, can solve the problems of nutrient loss, long fermentation time, low fermentation temperature, etc.

Active Publication Date: 2021-01-08
SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, domestic compost fermentation mainly uses mesophilic microbial agents for fermentation, which leads to low fermentation temperature (40-60°

Method used

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  • Methylobacterium thermophilum and application thereof in organic solid wastes
  • Methylobacterium thermophilum and application thereof in organic solid wastes
  • Methylobacterium thermophilum and application thereof in organic solid wastes

Examples

Experimental program
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preparation example Construction

[0027] The third aspect of the present invention provides the preparation method of the compost inoculant agent provided by the second aspect of the present invention. It is well known to those skilled in the art that the compost inoculant can be provided by inoculating microorganisms in the nutrient source and further carrying out fermentation treatment. Bacteria. For example, the preparation method of the compost inoculant may include: inoculating the above-mentioned Methylobacterium thermophilic on the substrate, and performing fermentation treatment.

[0028] In the preparation method of the compost inoculum agent provided by the present invention, M. thermophiles can usually be activated before the inoculation, so that a vigorous culture with sufficient inoculum quantity can be obtained. A suitable method for activating Methylobacterium thermophilic should be known to those skilled in the art, for example, the above-mentioned Methylobacterium thermophilus can be inoculate...

Embodiment 1

[0040] Isolation and Identification of Methylobacterium sp.CXJ-03

[0041] In a sterile environment, weigh 10g of bean dregs compost samples into a 100ml sterile water Erlenmeyer flask, place on a shaker and shake at 200r / min for 30min to fully disperse the samples, and then incubate at 75°C for 24 hours.

[0042] Take out the Erlenmeyer flask cultured above, and let it stand for 3 minutes in a sterile environment, then use a sterile pipette to draw 10ml of the supernatant and add it to 100ml of sterilized CYS medium, shake it well, and place it at 70°C placed on a shaker at 200r / min, enriched for 24 hours, and subcultured for 3 generations. The formula of CYS medium is as follows: tryptone 3g, yeast powder 2g, soluble starch 1g, NaCl 3g, CaCl 2 0.5g, MgCl 2 0.125g, FeSO 4 10 mg, 100 μL of trace element solution, add distilled water to make up to 1 L, adjust the pH to 7.5 with NaOH, autoclave at 121 °C for 20 min and cool to room temperature for storage. Wherein, the tr...

Embodiment 2

[0050] Determination of the Growth Temperature Range of Geobacillus toebii CXJ-01

[0051] A single colony of Methylobacterium sp.CXJ-03 obtained in Example 1 was inserted into a 250 mL Erlenmeyer flask containing 50 mL of CYS liquid medium, and cultured with shaking at 75° C. for 12 hours at a frequency of 160-180 rpm. The cultured bacterial liquid was centrifuged at 6000rpm for 5min to collect the bacterial cells. Use sterile water to blow and beat the bacteria evenly to adjust the OD of the bacteria solution 600 to about 0.1.

[0052] Take 0.1mL of the above bacteria solution, add it into a test tube containing 3mL of CYS liquid medium, and place the test tubes at 30°C, 40°C, 50°C, 60°C, 65°C, 70°C, 71°C, 73°C, 75°C, Shaking culture was carried out in water baths at 77°C, 79°C, and 80°C, and the culture time was 12 hours. Determination of the OD of the bacterial solution in the test tube 600 .

[0053] The result is as figure 1 As can be seen from the temperature grow...

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Abstract

The invention relates to the technical field of biology, in particular to methylobacterium thermophilum. The preservation number of the methylobacterium thermophilum is CGMCC No. 20326. The methylobacterium thermophilum can utilize heat generated by the fermentation of compost raw materials as a heat source for propagation and growth, the highest growth temperature is up to 77 DEG C or above, themethylobacterium thermophilum can quickly proliferate in a high-temperature composting environment, and organic matters are degraded to promote the composting process, do that the dual effects of heatenergy utilization and waste treatment are achieved; in addition, microbial inoculum prepared from bacterial strains has low water content, so that storage and transportation are facilitated; the microbial inoculum can be directly mixed with a stacked material during use, so that operation is convenient, and the water content of the material is not increased; and the microbial inoculum is a goodwet-based material conditioner, and has wide application value.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a thermophilic Methylobacterium. Background technique [0002] Composting is considered to be a cost-effective technology for treating organic waste, which can convert the organic matter in organic waste into organic acids that can be effectively used by plants, thus becoming a good fertilizer for plants. However, at present, domestic compost fermentation mainly uses mesophilic microbial agents for fermentation, which leads to low fermentation temperature (40-60°C), long fermentation time (2-3 months), and prone to incomplete killing of diseases and insect pests, insufficient fermentation, Nutrient loss and other issues. In order to overcome the shortcomings of traditional composting technology, a new type of high-temperature aerobic composting fermentation technology has emerged in recent years, which can improve the efficiency of biological transformation through high temperature....

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F15/00C05F17/20C05F11/08C12R1/01
CPCC12N1/20C05F3/00C05F17/20C05F11/08C12R2001/01C12N1/205C05F5/002Y02W30/40
Inventor 赵志军陈枭嘉史吉平姜标王晨阳李沁雨李立马新新
Owner SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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