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Wastewater treating microbial agent and preparation method thereof

A microbial agent and wastewater treatment technology, which is applied in the field of environmental microorganisms to achieve good performance, high biological activity, and strong impact resistance

Active Publication Date: 2014-08-20
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition to solving the problems existing in the existing short-range nitrification and denitrification, the microbial agent can be added to various biochemical reaction structures to improve the composition of microorganisms and increase the efficiency of nitrogen removal. of organic pollutants

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The preparation method of embodiment 1 microbial bacterial agent

[0026] 1. Bacterial activation: The formula of the activating medium of Cochlear palustris FSDN-A is: beef extract: 8g / L, peptone: 12g / L, NaNO 2 : 0.5g / L, KNO 3 : 0.5g / L; the activation medium formula of Arthrobacter FDN-1 and Flavobacterium waterii FDN-2 is: beef extract: 5g / L, peptone: 10g / L, NaNO 2 : 1g / L, add 2.0% agar; the activation medium formula of denitrification paracoccus DN-3 is: KNO 3 : 1g / L, sodium succinate: 8g / L, KH 2 PO 4 :1g / L, FeCL 2 : 0.5g / L, add 2.0% agar; the activation medium formula of Methylobacterium SDN-3 is: ammonium sulfate: 0.5g / L, methanol: 0.75mL / L, KH 2 PO 4 :1g / L, FeCL 2 :0.5g / L,; add 2% agar. After spreading evenly on the plate, place it in a constant temperature incubator at a temperature of 30°C for activation.

[0027] 2. Use the inoculation loop to scrape the bacteria on the plate and inoculate them in the corresponding liquid culture solution respectivel...

Embodiment 2

[0030] Embodiment 2 Application in a certain wastewater treatment system

[0031] A wastewater treatment system influent NH 3 -The average concentration of N is 320mg / L, the average concentration of total nitrogen (TN) is 512mg / L, COD Cr The average concentration is 661mg / L. During operation, the average removal rate of ammonia nitrogen is 70%, the average removal rate of total nitrogen is only 48%, and the average removal rate of COD is 69%. Take a certain amount of liquid bacterial suspension A and add it to the aeration tank at one time according to the dosage of 5% (volume). After 30 days of operation, the average removal rate of ammonia nitrogen is 92%, the average removal rate of total nitrogen is 87%, and the COD The average removal rate is 94%, and the sludge concentration is 5% lower than before adding the bacterial agent. The addition of the bacterial agent improves the removal rate of total nitrogen and COD in the wastewater. At the same time, the sludge concent...

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Abstract

The invention discloses a wastewater treatment microbial agent and a preparation method thereof. The microbial agent contains Kocuriapalustris FSDN-A, Arthrobacter creatinolyticus FDN-1, Flavobacterium mizutaii FDN- 2. Paracoccus denitrificans DN-3 and Methylobacteriumphyllosphaerae SDN-3, the deposit registration numbers of the five strains are CGMCC No.5061, CGMCC No.3657, CGMCC No.3659, and CGMCC No. 3658 and CGMCC No.3660. The microbial bacterial agent can realize the removal of ammonia nitrogen, total nitrogen and CODcr in the same reactor, has good wastewater treatment effect, and can realize short-range nitrification and denitrification or simultaneous nitrification and denitrification denitrification while removing COD. Corkia palustris FSDN-A can utilize two kinds of nitrogen oxides, nitrous nitrogen and nitrate nitrogen, which can make the wastewater treatment system denitrify more thoroughly, and the value-added speed is slow in the process of competing for substrates, which is conducive to the reduction of sludge. Further broaden the scope of application of microbial agents.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and in particular relates to a microbial bacterial agent for wastewater treatment and a preparation method thereof. The bacterial agent can complete the removal of ammonia nitrogen, total nitrogen and CODcr, and realize the industrialization and production of microbial bacterial agents for wastewater treatment. commoditization. Background technique [0002] Biological denitrification is one of the more cost-effective methods to solve nitrogen pollution. Whether it is a traditional microbial-attached sewage treatment structure or some newly developed high-efficiency biofilm treatment systems, the microorganisms responsible for denitrification are mainly autotrophic nitrifying bacteria. The proliferation rate of autotrophic bacteria is slow, they cannot compete with heterotrophic bacteria in the mixed culture activated sludge system, it is difficult to obtain high biomass, and the nitri...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00C12N1/20C02F3/34C12R1/01C12R1/06C12R1/20
Inventor 高会杰孙丹凤许谦
Owner CHINA PETROLEUM & CHEM CORP
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