Resveratrol composite bacterial agent for treating high-concentration pahs wastewater and its application method

A technology of resveratrol and compound bacterial agents, applied in the direction of microorganism-based methods, chemical instruments and methods, biological water/sewage treatment, etc., can solve the problem of low degradation efficiency of PAHs

Active Publication Date: 2021-02-26
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

The use of microorganisms to degrade PAHs in water is a simple and common method, but the degradation efficiency of microorganisms in high concentrations of PAHs in water is generally not high

Method used

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  • Resveratrol composite bacterial agent for treating high-concentration pahs wastewater and its application method
  • Resveratrol composite bacterial agent for treating high-concentration pahs wastewater and its application method
  • Resveratrol composite bacterial agent for treating high-concentration pahs wastewater and its application method

Examples

Experimental program
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Effect test

Embodiment 1

[0050] Treatment of phenanthrene-containing wastewater with resveratrol-Methylobacterium complex bacterial agent. The dosage of the bacterial agent is waste water: agent 1: agent 2 volume ratio 200:1:1. The results showed that after 24 hours of treatment, the removal rate of phenanthrene reached 48.5%, which was 336% higher than that of no compound bacterial agent, and 38.3% higher than that of Methylobacterium alone. Analyze the removal rate of polycyclic aromatic hydrocarbons by high performance liquid chromatography (HPLC), figure 2 is the result graph of the analytical instrument.

Embodiment 2

[0052] Treatment of phenanthrene-containing wastewater with resveratrol-Methylobacterium complex bacterial agent. The dosage of the bacterial agent is waste water: agent 1: agent 2 volume ratio 100:0.5:1. The results showed that the removal rate of phenanthrene reached 51.2% after 48 hours of treatment, which was 201% higher than that of no bacterial agent, and 23.7% higher than that of Methylobacterium alone. The removal rate of polycyclic aromatic hydrocarbons was analyzed by HPLC, image 3 is the result graph of the analytical instrument.

Embodiment 3

[0054] Treatment of wastewater containing phenanthrene and acenaphthene with resveratrol-Methylobacterium complex bacterial agent. The dosage of the bacterial agent is waste water: agent 1: agent 2 volume ratio 200:1:1. After 48 hours of treatment, the removal rates of phenanthrene and acenaphthene reached 57.0% and 61.5%, respectively, which were 235% and 232% higher than those without bacterial agents, and 35.7% and 27.3% higher than those with Methylobacterium alone. The removal rate of polycyclic aromatic hydrocarbons was analyzed by HPLC, Figure 4 is the result graph of the analytical instrument.

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Abstract

The invention discloses a resveratrol composite microbial agent for treating high concentration PAHs wastewater and a usage method thereof. The composite microbial agent comprises: a reagent I and a reagent II; the reagent I is: an methylobacterium suspension diluted with an inorganic salt culture medium; the reagent II is: a mixed solution of an inorganic salt and resveratrol; the usage method includes the following steps: step 1. culturing and acclimating methylobacterium; step 2. preparing the methylobacterium suspension; step 3. preparing a resveratrol solution; step 4. uniformly mixing wastewater, the methylobacterium, and the resveratrol solutionin proportion; and step 5. filtering out water; and the microbial agent and the usage method find that resveratrol can significantly promotedegradation of activity of a PAHs key enzyme in an endophytic body, thereby promoting degradation and removal of PAHs in water by endophytes; and the microbial agent and the usage method provide a new method with high efficiency and simple operation for efficient biological treatment of the high concentration PAHs wastewater.

Description

technical field [0001] The invention relates to the technical field of waste water treatment, in particular to a resveratrol composite bacterial agent for treating PAHs waste water and a use method thereof. Background technique [0002] With the rapid development of modern economy and industry and agriculture, associated organic pollutants such as pesticides, chemical fertilizers and industrial wastes continue to enter the environment, thereby polluting the environment, destroying the ecological balance, and endangering human health through the food chain. Among them, polycyclic aromatic hydrocarbons (polycyclic aromatic hydrocarbons, PAHs) are typical persistent organic pollutants widely present in the environment. The efficient removal of PAHs in wastewater has always been one of the hotspots in environmental science and water treatment engineering research. The use of microorganisms to degrade PAHs in water is a simple and common method, but the degradation efficiency of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/32
CPCC02F3/34C02F2101/327C12N1/20
Inventor 吕黎柴奇伟李渊蔺孟婷马玉龙王至涵何闪英杨春平
Owner ZHEJIANG GONGSHANG UNIVERSITY
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