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Methylorubrum rhodesianum M520 and application thereof

A technology of Methylobacterium rosenbergii and bacteria agents, applied in the field of microorganisms, can solve the problems of rare Methylobacterium root-knot nematode disease prevention and promotion of plant growth, etc., to achieve rich control methods, high safety, and improve land utilization and the effect on vegetable yield

Active Publication Date: 2020-02-21
MOON (GUANGZHOU) BIOTECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Patent US5512069A discloses the use of Methylobacterium for seeds, and patent US5961687A discloses the use of Methylobacterium for leaves, but there are few applications of Methylobacterium for the control of crop root-knot nematode disease and the promotion of plant growth. research report

Method used

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  • Methylorubrum rhodesianum M520 and application thereof
  • Methylorubrum rhodesianum M520 and application thereof
  • Methylorubrum rhodesianum M520 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Isolation, purification and identification of Methylobacterium M520 strain

[0026] Methylobacteria include the following groups: Methylobacterium aminophagosus, Methylobacterium extorquens, M. flexneri, M. mesophilus, M. radiodurans, M. rosenbergii, A. nodosum M. phyllosphaerae, M. phyllosphaerae, M. organophilus, M. CM4 strain, M. thiocyanobacterium and M. oryzae.

[0027] 1. Isolation and purification of Methylobacterium M520 strain

[0028] The samples were collected from sweet potato leaves collected from farmland in Fujian Province.

[0029] Weigh 1g of sweet potato leaves, add them to sterilized 100mL Tween water 80 with a concentration of 0.1%, crush the leaves with a hand-held crusher, and dilute the leaf liquid gradient to 10 -2 、10 -3 concentration, and then spread it on AMS solid inorganic salt medium, and culture it upside down in a constant temperature incubator at 28°C for 5 days. Inverted culture in medium for 5 days, then observe the colo...

Embodiment 2

[0035] Embodiment 2: the effect of the crude extract of the metabolism of Methylobacterium M520 bacterial strain to kill vegetable root-knot nematode

[0036] 1. Preparation of nematodes for experiment

[0037] Root-knot nematodes were collected from the greenhouse of the Vegetable and Flower Research Institute of the Chinese Academy of Agricultural Sciences. Take out the root system of peppers with root-knot nematode disease, gently rinse with water, carefully remove the egg mass from the surface of the root system, put it in 0.5% sodium hypochlorite for disinfection for 3 minutes, then rinse it with sterile water for 3 times, and put it in a small amount of sterile water. cultured in a 25°C incubator, and collected the hatched second-instar larvae of root-knot nematodes at 24h-48h, suspended in sterile water for experimental research.

[0038] 2. Preparation of crude metabolic extract of Methylobacterium M520 strain

[0039] The preparation method of the metabolic crude ex...

Embodiment 3

[0054] Embodiment 3: Methylobacterium M520 bacterial strain fermented culture is in the effect of preventing and treating root-knot nematode disease of potted vegetables

[0055] 1. Preparation of fermented cultures of nematodes and Methylobacterium M520 strains for the test

[0056] Preparation of root-knot nematodes: root-knot nematodes were collected from the greenhouse of the Vegetable and Flower Research Institute of the Chinese Academy of Agricultural Sciences. Take out the root system of the root-knot nematode-infested pepper, rinse it gently with water, carefully remove the egg mass from the surface of the root system, put it in 0.5% sodium hypochlorite for disinfection for 3 minutes, then rinse it with sterile water for 3 times, and put it in a container containing a small amount of sterile water. Culture in a petri dish at 25°C in an incubator, collect hatched second-instar larvae of root-knot nematode within 24h-48h, suspend them with sterile water for experimental ...

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Abstract

The invention provides a strain of methylorubrum rhodesianum M520. The methylorubrum rhodesianum M520 is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number isGDMCC No.60729. The invention also provides an application of the M520 strain to preparation of a preparation for preventing and controlling root-knot nematodes or promotion of plant growth. When a metabolism crude extract of the methylorubrum rhodesianum M520 strain is applied to the root-knot nematodes, the lethal rate of the root-knot nematodes exceeds 96.6%; and in addition, a fermentation culture of the strain has an obvious promotion effect on growth of vegetable plants while effectively preventing and controlling the root-knot nematodes of potted vegetables, the use range and efficacy of methylobacterium are expanded, the prevention and control measures of a root-knot nematode disease is enriched, and the growth rate and vegetable yield of the vegetable plants are improved.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a Methylorubrum rhodesianum M520 and an application thereof. Background technique [0002] Vegetable root-knot nematode disease, also known as "tumor disease", is a common vegetable disease caused by root-knot nematodes. In recent years, due to the expansion of the area of ​​vegetables in greenhouses, suitable environmental conditions have been provided for the occurrence of the disease, making nematodes more and more serious, especially cucumbers, tomatoes, and celery, which are continuously cropped in greenhouses, and the annual losses are as high as tens of billion. Due to the scarcity of vegetable germplasm resources resistant to root-knot nematode disease, the main way to control vegetable root-knot nematode disease is still chemical control, such as abamectin and dameron. Although these chemicals can effectively kill root-knot nematodes, they will also ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/20A01P5/00A01P21/00C12R1/01
CPCA01N63/00C12N1/20C12N1/205C12R2001/01
Inventor 李彦王科晶余昕彤梁锏文茆振川凌键殷楠蒋先芝王琳谢丙炎
Owner MOON (GUANGZHOU) BIOTECH CO LTD
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