Degrading bacterium for TC (tetracycline) antibiotics and application of degrading bacterium

A tetracycline and antibiotic technology, applied in bacteria, microorganisms, microorganisms, etc., can solve the problems of undiscovered degrading engineering bacteria and environmental damage

Active Publication Date: 2017-12-29
众心通慧(厦门)生态环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no degrading engineering bacteria that can simultaneously degrade three tetracycline antibiotics (TC, OTC, and CTC) have been found so far, and the current environmental pollution status is often a complex pollution system in which multiple antibiotics exist at the same time, rather than a simple system of a single antibiotic. Therefore, a degrading engineering bacterium that can simultaneously degrade a variety of tetracycline antibiotics was screened out, and it was made into a microbial agent and applied to an environment actually polluted by one or more antibiotics to improve the degradation and removal of antibiotic pollutants. This has important practical significance for reducing the risks of environmental damage, ecological imbalance and reducing the production of resistant bacteria caused by antibiotic pollution.

Method used

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  • Degrading bacterium for TC (tetracycline) antibiotics and application of degrading bacterium
  • Degrading bacterium for TC (tetracycline) antibiotics and application of degrading bacterium
  • Degrading bacterium for TC (tetracycline) antibiotics and application of degrading bacterium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Inoculate the suspension of degrading bacteria YWF1 pre-cultivated in sodium acetate as the carbon source into the liquid medium with 10 mg / L TC, OTC and CTC as the sole carbon source, and place it in a shaker at 37 °C to Culture at 150 rpm in the dark. Sampling was taken at regular intervals, and changes in the concentration of antibiotics were determined by high performance liquid chromatography. attached by figure 2 It can be seen that the removal effects of degrading bacteria YWF1 when using TC, OTC and CTC as the sole carbon source are as follows: Compared with the sterile control group, within 36 h, the removal efficiencies of degrading bacteria YWF1 on 10 mg / LCTC, OTC and TC were respectively are 99.00% ± 0.01%, 62.44% ± 2.19% and 7.37% ± 4.87%. The above results indicated that the degrading bacteria YWF1 could achieve good degradation in the presence of a single tetracycline antibiotic.

[0023] In the above experiment, the liquid medium with 10 mg / L TC, OTC...

Embodiment 2

[0025]Inoculate the suspension of degrading bacteria YWF1 pre-cultivated in sodium acetate as carbon source into liquid medium with TC, OTC and CTC as mixed carbon source (the total concentration of tetracycline antibiotics is 30 mg / L, pH is 7.0), and the It was cultured in a shaker at 37 °C at 150 rpm in the dark. Samples were taken regularly, and the concentration changes of the three antibiotics were determined by high-performance liquid chromatography, and the OD was measured by a UV spectrophotometer at the same time. 600 To study the growth of degrading bacteria YWF1 with mixed tetracycline antibiotics as the only substrate. attached by image 3 It can be seen that the degrading bacteria YWF1 can grow in the liquid medium in which the three tetracyclines co-exist, with the three tetracycline antibiotics as the mixed carbon source. The removal rates of CTC, OTC and TC within 36 hours of the degrading bacteria YWF1 in the mixed antibiotic treatment group were 83.18% ± 0....

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Abstract

The invention relates to a degrading bacterium Methylobacterium sp. YWF1 capable of degrading three TC (tetracycline) antibiotics, namely, TC, OTC (oxytetracycline) and CTC (Chlorotetracycline) simultaneously. The degrading bacterium YWF1 is identified as Methylobacterium and was preserved in CGMCC (China General Microbiological Culture Collection Center) on April 17, 2017 with preservation number being CGMCCNo. 14040. Methylobacterium sp. YWF1 can simultaneously degrade the three TC antibiotics, namely, TC, OTC and CTC, a microbial agent suspension can be prepared from the bacterium to be applied to degrading removal of TC antibiotic pollutants in different environment media such as livestock wastewater, TC pharmaceutical enterprise wastewater, medical wastewater or soil, and has good industrial application prospect and environmental benefits.

Description

technical field [0001] The invention relates to the technical field of environmental protection and microbial degradation of new organic pollutants, in particular to a tetracycline antibiotic-degrading bacterium YWF1 and its application. Background technique [0002] Tetracycline antibiotics are a class of antibiotics extracted from Streptomyces culture fluid or semi-synthesized. They are named for the four rings in their molecular structure, so they are called tetracyclines. Among them, Tetracycline (Tetracycline, TC), Oxytetracycline (Oxytetracycline, OTC) and Chlorotetracycline (Chlorotetracycline, CTC) are the three main antibiotics widely used in tetracycline antibiotics. The mechanism of action of tetracycline antibiotics is to pass through the hydrophilic pores of the outer membrane, enter the cell from the energy-dependent transfer system on the inner membrane, specifically bind to the ribosomal 30S subunit, prevent aminoacyl-tRNA from linking, and thereby inhibit pe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/38
CPCC02F3/34C02F2101/38C12N1/20C12N1/205C12R2001/01
Inventor 肖勇严伟富冮海银赵峰
Owner 众心通慧(厦门)生态环保科技有限公司
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