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Bacterium for degrading herbicide imazethapyr and application of bacterium

A technology of pusher and bacterial agent, applied in the direction of bacteria, microorganism-based methods, and restoration of contaminated soil, etc., can solve problems such as slow natural degradation, decline in sugar beet planting, and phytotoxicity

Active Publication Date: 2014-08-13
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Second, there are still phytotoxicity 3-4 years after herbicide application, which leads to the decline of sugar beet planting industry
[0006] The degradation of herbicide residues in nature is mainly accomplished by microorganisms in the soil, but the natural degradation is very slow

Method used

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  • Bacterium for degrading herbicide imazethapyr and application of bacterium
  • Bacterium for degrading herbicide imazethapyr and application of bacterium
  • Bacterium for degrading herbicide imazethapyr and application of bacterium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1, the isolation and identification of Methylobacterium sp. PST1991CGMCC No.7773

[0039] 1. Isolation of Pushte degrading bacteria PST1991

[0040] Add 10g of soil samples (collected from the farmland polluted by the herbicide Pursit in Beijing, China) to 100mL enrichment medium containing 200 mg / L of Pursit, and culture at 28°C and 200r / min for 7 days to enrich and degrade Pursit bacteria. After the first round of enrichment, 10 mL of the enrichment solution was transferred to 100 mL of the enrichment medium containing 400 mg / L of Prostil, and the culture was continued for 7 days for the second round of enrichment. Such continuous enrichment and culture were carried out 5 times, and the concentration of Pustetra was 200, 400, 600, 800 and 1000 mg / L in sequence. After the enrichment is completed, take the enrichment solution and evenly spread it on the inorganic salt solid medium containing 1000 mg / L of Prostil, and incubate at a constant temperature of 28°C ...

Embodiment 2

[0063] Embodiment 2, Methylobacterium sp. (Methylobacterium sp.) PST1991CGMCC No.7773 Degradation Pushite Ability Quantitative Determination

[0064] 1. Preparation of standard curve for Pushett determination

[0065] Accurately weigh 10.0mg of Pushte standard (purchased from Fluka) into a 10mL volumetric flask, dissolve it with a small amount of methanol, place the volumetric flask in an ultrasonic bath and vibrate for 10min, then dilute to 10mL with methanol, shake well, and prepare Become 1000mg / L Pushite mother liquor. Continue to dilute to prepare 10mg / L mother solution, and then prepare 1, 2, 4, 6, 8, 10mg / L serial standard solutions. High-performance liquid chromatography (HPLC) was used to measure the peak areas of different concentrations of Pushite standards, with three repetitions. With the concentration of Puszt as the abscissa and the peak area as the y-coordinate, draw the Puszt standard curve.

[0066] The detection conditions are as follows:

[0067] Detect...

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Abstract

The invention discloses a bacterium for degrading a herbicide imazethapyr and application of the bacterium. The bacterium for degrading imazethapyr is methylobacterium sp. PST1991, which is preserved in China General Microbiological Culture Collection Center by a preservation number of CGMCC No.7773. The methylobacterium sp. PST1991 CGMCC No.7773 reaches a degrading rate of 71.06% on 8mg / L of imazethapyr in an organic salt culture medium within 7d, showing that the strain can effectively degrade the imazethapyr; the bacterium has a wide application prospect in an aspect of repairing imazethapyr pollution of soil.

Description

technical field [0001] The invention relates to a bacterium for degrading the herbicide pushit and its application. Background technique [0002] Pursuit, also known as imazethapyr, English name Pursuit (imazethapyr–ammonium), chemical name (RS)-5-ethyl-2-(4-isopropyl-4-methyl-5-oxo-2 -imidazolin-2-yl) nicotinic acid, chemical structure as shown in formula 1: [0003] [0004] Pushit is an imidazolinone herbicide and an inhibitor of side chain amino acid synthesis. It can be applied before or after emergence. It can be applied to grass weeds and certain broad-leaved weeds such as amaranth in soybean fields and other legume farmlands. , Polygonum, Chenopodium, Solanum nigrum, Cocklebur, Barnyardgrass, Setaria, crabgrass, millet, etc. have excellent control effects. [0005] Pushit is a long-acting herbicide, which has the advantages of good herbicidal effect, wide herbicidal spectrum, less dosage, convenient use, and low cost of medication, but they remain in the soil fo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A62D3/02B09C1/10C12R1/01A62D101/04A62D101/28
Inventor 孙建光吕翻洋王文博徐晶
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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