Nitrous acid type biological denitrogenation microbial agent and application thereof

A technology of microbial inoculum and nitrous acid is applied in the field of nitrous acid type biological denitrification inoculant to achieve the effects of reducing operating costs, improving the effect and strengthening the denitrification effect.

Active Publication Date: 2012-05-23
CHINA PETROLEUM & CHEM CORP +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition to solving the problems existing in the existing short-range nitrification and denitrification, the microbial agent can be added to variou

Method used

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  • Nitrous acid type biological denitrogenation microbial agent and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The preparation of embodiment 1 microbial bacterial agent

[0026] 1. Bacteria activation: The activation medium formula of Arthrobacter FDN-1 and Flavobacterium waterii FDN-2 is: beef extract: 5g / L, peptone: 10g / L, NaNO 2 : 1g / L, add 2.0% agar; the activation medium formula of denitrification paracoccus DN-3 is: KNO 3 : 1g / L, sodium succinate: 8g / L, KH 2 PO 4 : 1g / L, FeCL 2 : 0.5g / L, add 2.0% agar; the activation medium formula of Methylobacterium SDN-3 is: ammonium sulfate: 0.5g / L, methanol: 0.75mL / L, KH 2 PO 4 : 1g / L, FeCL 2 : 0.5g / L,; add 2% agar. After spreading evenly on the plate, place it in a constant temperature incubator at a temperature of 30°C for activation.

[0027] 2. Use the inoculation loop to scrape the bacteria on the plate and inoculate them in the corresponding liquid culture solution respectively, and culture them with shaking under aerobic conditions of 25-35°C and 150-240rpm for 1-3 days until the logarithmic growth phase, and obtain Liq...

Embodiment 2

[0030] Embodiment 2 Application of bacterial agent of the present invention in nitrogen fertilizer wastewater denitrification process

[0031] Get a certain amount of bacterial suspension A that Example 1 obtains and inoculate in the aeration reactor according to the inoculum size of 5% (volume), at 30 ℃ of temperature, pH 7.7, DO (dissolved oxygen) is 0.9~2.9mg L -1 Under the conditions, the treatment of ammonia-containing wastewater generated in the process of nitrogen fertilizer production, NH 3 -The average concentration of N is 450mg / L, the average concentration of total nitrogen (TN) is 500mg / L, COD Cr The average concentration is 150mg / L. Methanol was added as a carbon source during the treatment. After treatment, the effluent NH 3 The concentrations of -N and TN are 5mg / L~14.7mg / L and 24mg / L~40.7mg / L respectively, and the removal rates can reach 19.77mg / (L.h) and 20.88mg / (L.h) respectively. NH 3 -N, TN and COD removal rates are greater than 96.7%, 91.8% and 90%, re...

Embodiment 3

[0032] Example 3 Application of the bacterial agent of the present invention in the denitrification process of monosodium glutamate wastewater

[0033] The experiment was carried out in three 100L SBR reactors, the concentration of ammonia nitrogen was 300mg / L-450mg / L, and the average COD concentration was 1500mg / L. First add the same concentration of nitrifying activated sludge in three reactors, one of which adds the bacterial suspension C (volume ratio is 2%) of Example 1, and one adds SDN-3 (volume ratio is 2%) bacteria liquid, the other only added activated sludge. At a temperature of 28°C, pH 8.0, DO (dissolved oxygen) is 2.5mg·L -1 Treatment of monosodium glutamate wastewater under the same conditions, the treatment time is 22h. From the effect comparison in Table 1, when only activated sludge is added, the removal rates of ammonia nitrogen and total nitrogen are 52.9% and 43.7%, respectively, and the removal effect of ammonia nitrogen and total nitrogen is the best a...

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Abstract

The present invention discloses a short-cut nitrification-denitrification microbial agent. The microbial agent contains Arthrobacter creatinolyticus FDN 1, Flavobacterium mizutaii FDN 2, Paracoccus denitrificars DN 3 and Methylobacterium phyllosphaerae SDN3. The preservation register numbers of the four strains respectively are CGMCC No.3657, CGMCC No.3659, CGMCC No.3658 and CGMCC No.3660. With the microbial agent, the ammonia nitrogen removing, the total nitrogen removing and the CODcr removing in the same reactor can be achieved, the wastewater treatment effect is good, and the microbial agent is especially suitable for the purification treatment of the wastewater containing the ammonia nitrogen so as to really achieve the short-cut nitrification-denitrification or simultaneous nitrification and denitrification.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and in particular relates to a nitrous acid-type biological denitrification bacterial agent and its application. The microbial bacterial agent can realize the industrialization and commercialization of the microbial bacterial agent for denitrification of ammonia-containing wastewater. Background technique [0002] Biological denitrification is one of the more cost-effective methods to solve nitrogen pollution. Whether it is a traditional microbial-attached sewage treatment structure or some newly developed high-efficiency biofilm treatment systems, the microorganisms responsible for denitrification are mainly autotrophic nitrifying bacteria. The proliferation rate of autotrophic bacteria is slow, they cannot compete with heterotrophic bacteria in the mixed culture activated sludge system, it is difficult to obtain high biomass, and the nitrification efficiency is low, resulting in weak...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C02F3/30C12R1/06
Inventor 高会杰黎元生李志瑞许谦
Owner CHINA PETROLEUM & CHEM CORP
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