A kind of Methylobacterium thermophilus and its application in organic solid waste

A Methylobacterium and substrate technology, applied in the biological field, can solve problems such as incomplete killing of diseases and insect pests, loss of nutrients, insufficient fermentation, etc.

Active Publication Date: 2022-07-05
SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, domestic compost fermentation mainly uses mesophilic microbial agents for fermentation, which leads to low fermentation temperature (40-60°C), long fermentation time (2-3 months), and prone to incomplete killing of diseases and insect pests, insufficient fermentation, Nutrient loss and other issues

Method used

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  • A kind of Methylobacterium thermophilus and its application in organic solid waste
  • A kind of Methylobacterium thermophilus and its application in organic solid waste
  • A kind of Methylobacterium thermophilus and its application in organic solid waste

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preparation example Construction

[0027] The third aspect of the present invention provides the preparation method of the compost inoculant provided by the second aspect of the present invention. It is well known to those skilled in the art that the compost inoculum can usually be provided by inoculating microorganisms on a nutrient source and further performing fermentation treatment. Bacterial agent. For example, the preparation method of a compost inoculant may include: inoculating the above-mentioned Methylobacter thermophilus on a substrate, and fermenting.

[0028] In the preparation method of the compost inoculant provided by the present invention, before inoculation, M. thermophilus can also be usually activated, so that a vigorous culture with sufficient inoculation quantity can be obtained. Suitable methods for activating M. thermophilus should be known to those skilled in the art, for example, the above-mentioned M. thermophilus can be inoculated into a suitable medium (for example, CYS liquid mediu...

Embodiment 1

[0040] Isolation and identification of Methylobacterium sp.CXJ-03

[0041] In a sterile environment, weigh 10g of bean dregs compost sample into a 100ml sterile water conical flask, place it on a shaker at 200r / min for 30min to fully disperse the sample, and then incubate at 75°C for 24 hours.

[0042] Take out the above cultured conical flask, let it stand for 3 minutes in a sterile environment, then use a sterile pipette to suck 10ml of the supernatant and add it to 100ml of sterilized CYS medium, shake it well, and place it at 70°C 200 r / min on a shaking table, enrichment culture for 24 hours, so, subculture for 3 generations. The formula of CYS medium is as follows: tryptone 3g, yeast powder 2g, soluble starch 1g, NaCl 3g, CaCl 2 0.5g, MgCl 2 0.125g, FeSO 4 10 mg, 100 μL of trace element solution, add distilled water to make up to 1 L, adjust pH to 7.5 with NaOH, sterilize by autoclaving at 121 °C for 20 min, and cool to room temperature for storage. Among them, the...

Embodiment 2

[0050] Determination of Growth Temperature Range of Methylobacterium thermophilus Geobacillus toebii CXJ-01

[0051] The single colony of Methylobacterium sp. CXJ-03 obtained in Example 1 was inserted into a 250 mL conical flask containing 50 mL of CYS liquid medium, and cultured with shaking at 75° C. for 12 h at a shaking frequency of 160-180 rpm. The cultured bacterial liquid was centrifuged at 6000 rpm for 5 min by a centrifuge, and the bacterial cells were collected. Use sterile water to blow and beat the bacteria evenly to adjust the OD of the bacteria solution 600 to about 0.1.

[0052] Take 0.1 mL of the above bacterial liquid, add it to a test tube containing 3 mL of CYS liquid medium, and put the test tube into 30°C, 40°C, 50°C, 60°C, 65°C, 70°C, 71°C, 73°C, 75°C, Shaking culture was performed in a water bath at 77°C, 79°C, and 80°C for 12 h. Determination of OD of bacterial liquid in test tube 600 .

[0053] The result is as figure 1 As shown, the temperature ...

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Abstract

The invention relates to the field of biotechnology, in particular to a Methylobacter thermophilus. The present invention provides a Methylobacter thermophilus, the deposit number of which is CGMCC No.20326. The M. thermophilus provided by the invention can use the heat of self-fermentation of compost raw materials as a heat source to reproduce and grow, and the maximum growth temperature is as high as 77°C or more, can rapidly multiply in a high-temperature composting environment, degrade organic matter, promote the composting process, and achieve thermal energy. The dual effect of utilization and waste disposal. In addition, the inoculum prepared by this strain has a low moisture content, which is beneficial to storage and transportation. It can be directly mixed with the stacking material when used. It is easy to operate and will not increase the moisture content of the material. It is also a good wet-based material conditioner. , has a wide range of application value.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a Methylobacter thermophilus. Background technique [0002] Composting is considered as a cost-effective technology for the treatment of organic waste, which converts the organic matter in the organic waste into organic acids that can be effectively used by plants, thus becoming a good fertilizer for plants. However, at present, domestic compost fermentation mainly adopts mesophilic microbial inoculum fermentation, which leads to low fermentation temperature (40-60 ° C), long fermentation time (2-3 months), and prone to incomplete killing of pests and diseases, insufficient fermentation, Nutrient loss, etc. In order to overcome the shortcomings of traditional composting technology, a new type of high-temperature aerobic composting fermentation technology has emerged in recent years, which can improve the biotransformation efficiency through high temperature. Therefore, adding therm...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F15/00C05F17/20C05F11/08C12R1/01
CPCC12N1/20C05F3/00C05F17/20C05F11/08C12R2001/01C12N1/205C05F5/002Y02W30/40
Inventor 赵志军陈枭嘉史吉平姜标王晨阳李沁雨李立马新新
Owner SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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