Microbial agent for efficiently degrading formaldehyde and preparation method of microbial agent

A microbial bacterial agent and microbial culture technology, which is applied in the field of microbial bacterial agent for efficient degradation of formaldehyde and its preparation, can solve the problems of inability to achieve efficient degradation of formaldehyde

Active Publication Date: 2020-12-29
北京首诚田园科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned invention patents all involve microbial agents capable of degrading formaldehyde, but when the above-mentioned single microbial agent is used to degrade formaldehyde indoors, it is often impossible to efficiently degrade low-concentration formaldehyde

Method used

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  • Microbial agent for efficiently degrading formaldehyde and preparation method of microbial agent
  • Microbial agent for efficiently degrading formaldehyde and preparation method of microbial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The application provides a preparation method of a microbial agent for efficiently degrading formaldehyde, comprising the following steps: S1, configuring a solid medium A, the formula of which is: beef extract 3g / L, peptone 10g / L, sodium chloride 5g / L , potassium dihydrogen phosphate 0.6g / L, dipotassium hydrogen phosphate 0.85g / L, agar 20g / L, and trace element solution 550μL / L. The trace element solution is prepared by dissolving the following raw materials in deionized water: boric acid 6g / L, copper chloride 0.2g / L, cobalt chloride 4g / L, nickel chloride 0.4g / L, zinc sulfate 2g / L, chlorine Manganese chloride 0.6g / L.

[0042] Configure solid LB medium, its formula is: tryptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L, agar 20g / L.

[0043] Configure solid medium B, its formula is: sodium malate 1.0g / L, sodium acetate 1.0g / L, ammonium chloride 1.0g / L, potassium dihydrogen phosphate 1.0g / L, calcium chloride 0.1g / L, Sodium bicarbonate 2.9g / L, magnesium chloride 0....

Embodiment 2

[0051] The difference between this example and Example 1 is that in this example, in step S2, the three strains of Methylobacterium MR1, Pseudomonas IOFA1 and Rhodopseudomonas palustris LY-6 were inoculated in liquid medium A After incubation, culture in the dark until the total number of viable bacteria reached 2×10 9 cells / mL to obtain the microbial culture solution.

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Abstract

The invention discloses a microbial agent for efficiently degrading formaldehyde and a preparation method of the microbial agent, belongs to the technical field of indoor air purification, and solvesthe technical problem that an existing single microbial agent cannot efficiently degrade low-concentration formaldehyde. The preparation method of the microbial agent comprises the following steps: S1, activating Methylobacterium, Pseudomonas and Rhodopseudomonas palustris for standby application; S2, inoculating the activated Methylobacterium and activated Pseudomonas into a liquid culture mediumA for culturing for 12-18 h, then inoculating the activated Rhodopseudomonas palustris into the liquid culture medium A, and performing culturing in a dark environment with formaldehyde; and S3, centrifuging the microbial culture solution, and performing cell disruption, membrane filtration and drying on thalli to obtain the microbial agent. The microbial agent disclosed by the invention is usedfor degrading indoor formaldehyde, and has the advantage of efficiently degrading indoor low-concentration formaldehyde.

Description

technical field [0001] The present application relates to the technical field of indoor air purification, more specifically, it relates to a microbial agent for efficiently degrading formaldehyde and a preparation method thereof. Background technique [0002] Formaldehyde is a common extremely volatile organic compound (Volatile Organic Compounds, VOCs). As a basic chemical raw material, it is widely used in the fields of resin, leather, paint, coating and plastic. With the development of furniture manufacturing, interior building materials and other industries, adhesives and coatings made of organic raw materials containing formaldehyde will be used in furniture and decoration materials, and the formaldehyde will be continuously released into the indoor environment, causing indoor pollution. Formaldehyde content is too high. In addition, formaldehyde in furniture and interior decoration materials will continue to be released into the indoor environment for a long period of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B01D53/84B01D53/72C12R1/01C12R1/38
CPCC12N1/20B01D53/84B01D53/72B01D2258/06Y02A50/20
Inventor 于兴军
Owner 北京首诚田园科技发展有限公司
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