A kind of microbial inoculum for efficiently degrading formaldehyde and preparation method thereof

A microbial bacterial agent and microbial culture technology, which is applied in the field of microbial bacterial agent for efficiently degrading formaldehyde and its preparation, can solve the problems that formaldehyde cannot be efficiently degraded, and achieve the effect of improving the degradation rate of formaldehyde

Active Publication Date: 2022-07-12
北京首诚田园科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned invention patents all involve microbial agents capable of degrading formaldehyde, but when the above-mentioned single microbial agent is used to degrade formaldehyde indoors, it is often impossible to efficiently degrade low-concentration formaldehyde

Method used

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  • A kind of microbial inoculum for efficiently degrading formaldehyde and preparation method thereof
  • A kind of microbial inoculum for efficiently degrading formaldehyde and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0041] The present application provides a method for preparing a microbial inoculum for efficiently degrading formaldehyde, comprising the following steps: S1. Configuring a solid medium A, the formula of which is: beef extract 3g / L, peptone 10g / L, sodium chloride 5g / L , potassium dihydrogen phosphate 0.6g / L, dipotassium hydrogen phosphate 0.85g / L, agar 20g / L, and trace element solution 550μL / L. The trace element solution is prepared by dissolving the following raw materials in deionized water: boric acid 6g / L, copper chloride 0.2g / L, cobalt chloride 4g / L, nickel chloride 0.4g / L, zinc sulfate 2g / L, chlorine Manganese 0.6g / L.

[0042] A solid LB medium was prepared, and its formula was: tryptone 10 g / L, yeast extract 5 g / L, sodium chloride 10 g / L, and agar 20 g / L.

[0043] Configure solid medium B, its formula is: sodium malate 1.0g / L, sodium acetate 1.0g / L, ammonium chloride 1.0g / L, potassium dihydrogen phosphate 1.0g / L, calcium chloride 0.1g / L, Sodium bicarbonate 2.9g / L, ma...

Embodiment 2

[0051] The difference between this example and Example 1 is that in this example, in step S2, three strains of Methylobacter MR1, Pseudomonas IOFA1 and Rhodopseudomonas swampis LY-6 were inoculated in liquid medium A After the medium, culture in the dark until the total viable count reaches 2×10 9 A / mL to obtain a microbial culture solution.

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Abstract

The application discloses a microbial inoculant for efficiently degrading formaldehyde and a preparation method thereof, belonging to the technical field of indoor air purification, and solving the technical problem that the existing single microbial inoculant cannot efficiently degrade low-concentration formaldehyde. The preparation method of microbial inoculum comprises the following steps: S1, activate Methylobacterium, Pseudomonas and Rhodopseudomonas swamp, for standby use; S2, inoculate the activated Methylobacterium and the activated Pseudomonas into a liquid After culturing in medium A for 12-18 h, inoculate the activated Rhodopseudomonas swamp into liquid medium A, and cultivate in a dark environment with formaldehyde; S3, centrifuge the microbial culture solution, and remove the bacterial cells The microbial inoculum is obtained after the cells are broken, passed through the membrane and dried. The microbial inoculum of the present application is used for indoor formaldehyde degradation, and has the advantage of efficiently degrading indoor low-concentration formaldehyde.

Description

technical field [0001] The present application relates to the technical field of indoor air purification, and more particularly, to a microbial inoculant for efficiently degrading formaldehyde and a preparation method thereof. Background technique [0002] Formaldehyde is a common and highly volatile organic compound (Volatile Organic Compounds, VOCs). As a basic chemical raw material, it is widely used in the fields of resin, leather, paint, coating and plastic. With the development of furniture manufacturing, indoor building materials and other industries, adhesives and coatings made of organic raw materials containing formaldehyde will be used in furniture and decoration materials, and the formaldehyde will be continuously released into the indoor environment, causing indoor air pollution. Formaldehyde content is too high. In addition, formaldehyde in furniture and interior decoration materials will continue to be released into the indoor environment for a long period of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B01D53/84B01D53/72C12R1/01C12R1/38
CPCC12N1/20B01D53/84B01D53/72B01D2258/06Y02A50/20
Inventor 于兴军
Owner 北京首诚田园科技发展有限公司
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