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Methylobacterium, fermentation product thereof and application of methylobacterium in inhibition of growth of algae

A Methylobacillus and algae technology, applied in the field of microorganisms, can solve the problems of long running time, unsuitable for large-scale operation, secondary pollution, etc.

Active Publication Date: 2022-06-07
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

①Physical methods (sediment dredging, mechanical algae removal, and water diversion) have the advantages of being simple, not introducing secondary pollution, and preventing the invasion of foreign organisms. They are suitable for emergency measures, but they cannot eliminate the toxic algae released after they die. Toxic substance, high cost and not suitable for large area operation
②Chemical method (adding chemical agents to kill algae, adding lime to denitrify, etc.) has the advantages of simple operation, less dosage, quick effect, and reasonable economy, but it is easy to cause secondary pollution, which is harmful to the water environment, ecological environment and aquatic organisms. damage
③The ecological method (aquatic vegetation restoration, biological floating island technology) has the advantages of environmental and ecological benefits, but there is a risk of introducing alien species, and the operation is complicated and the operation time is long
However, current information on Methylobacterium ( Methylobacterium sp.) There are few reports on the inhibition of algae growth

Method used

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  • Methylobacterium, fermentation product thereof and application of methylobacterium in inhibition of growth of algae
  • Methylobacterium, fermentation product thereof and application of methylobacterium in inhibition of growth of algae
  • Methylobacterium, fermentation product thereof and application of methylobacterium in inhibition of growth of algae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Methylobacter ( Methylobacterium sp.) 12-2 isolation, identification and culture

[0022] Methylobacillus of the present invention ( Methylobacterium sp.) 12-2 was isolated and purified from Hongze Lake, South China Agricultural University, Tianhe District, Guangzhou City, Guangdong Province, and specifically includes the following steps:

[0023] (1) Separation: Collect water samples from Hongze Lake of South China Agricultural University and dilute them to 10 5 , 10 6 , 10 7 Then spread on algal powder inorganic salt medium (1 g / L K 2 HPO 4 , 0.5 g / L MgSO 4 , 0.5 g / L NH 4 Cl, 0.5 g / L KNO 3 , 0.1% trace elements, 0.1% vitamins, 1% high-purity spirulina powder (purchased from Wanbang Industrial Co., Ltd. in Henan), and the formula of trace elements is 10mL / L HCl, 1.5 g / L FeCl 2 ·4H 2 O, 190 mg / L CoCl 2 ·6H 2 O, 100 mg / L MnCl 2 ·4H 2 O, mg / L, 70 mg / L ZnCl 2 , 62 mg / L H 2 BO 4 , 36 mg / L Na 2 MoO 4 ·2H 2 O, 24 mg / L NiCl 2 ·6H 2 O, 17 mg / LC...

Embodiment 2

[0026] Example 2 Methylobacter ( Methylobacterium sp.) 12-2 broad-spectrum algal inhibition

[0027] Microcystis aeruginosa ( Microcystis aeruginosa ), Tetraphyllans oblique ( Tetradesmus obliquus ) and Chlorella pyrenoidosa ( Chlorella pyrenoidosa ), Cynocystis subcapitaris ( Raphidocelis subcapitata ) and silk algae ( Ulotrixsp.) were transferred to BG11 medium and cultured for 10 days, then the test strain Methylobacter 12-2 (refer to (3) culture method of Example 1, using the stable phase Methylobacter 12-2 bacterial liquid) Inoculated in BG11 medium containing Microcystis aeruginosa, Tetraphyllans obliquus, Chlorella pyrenoidosa, Cynocystis subcapitaris and Rhizoma algae (volume ratio of bacteria and algae liquid is 1:9), at 25 ℃, 12 Cultured for 4 d under the conditions of h day / 12 h night and 2500 lx, with 3 parallels in each group. According to the acetone extraction method in "Spectrophotometric Method for Determination of Chlorophyll in Water Quality", th...

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Abstract

The invention discloses methylobacterium, a fermentation product thereof and application of the methylobacterium to inhibition of algae growth, and belongs to the technical field of microorganisms. The preservation number of the methylobacterium 12-2 is GDMCC No: 62327, and the methylobacterium 12-2 has a remarkable algal inhibition effect on apis clostridium, chlorella pyrenoidosa and microcystis aeruginosa, so that the methylobacterium 12-2 is rich in algal inhibition spectrum and has very strong application potential in the aspect of biological agents for controlling algal blooms.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a methylobacter strain and its fermentation product and its application in inhibiting the growth of algae. Background technique [0002] Harmful algal blooms are increasing in frequency due to water eutrophication and climate warming. Algal blooms destroy water ecosystems, endanger aquaculture, threaten human health, and deteriorate water landscapes. They are the main targets of current surface water pollution prevention and control. At present, the methods of controlling algal blooms at home and abroad mainly include four kinds of physical methods, chemical methods, ecological methods and biological methods. ①Physical methods (dredging sediment, mechanical algae removal, diverting water and silting) have the advantages of simple method, no secondary pollution, and can prevent biological invasion from outside the area. They are suitable for emergency measures, but cannot ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/20A01P13/02A01P1/00C12R1/01
CPCC12N1/20A01N63/20Y02W10/10
Inventor 杨旭楠刘丛竹许玫英
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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