Synergistic prevention and treatment of beauveria bassiana SB038 and spinetoram on megalurothrips usitatus

A technology of spinosad and Beauveria bassiana, applied in the directions of pesticides, biocides, botanical equipment and methods, etc., can solve the problems of resistance, insignificant drug effect, etc., and achieve good control effects, The effect of no pollution and low drug resistance in the environment

Active Publication Date: 2021-07-23
SOUTH CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the research on the compounding effect of Beauveria bassiana and spinosad, the present invention finds that the compounded insecticides can show a significant synergistic effect, improve the control effect, and can significantly reduce chemical pesticides. The use of a single dose reduces environmental pollution and effectively solves the problems of existing pesticides that are prone to resistance and ineffective.

Method used

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  • Synergistic prevention and treatment of beauveria bassiana SB038 and spinetoram on megalurothrips usitatus
  • Synergistic prevention and treatment of beauveria bassiana SB038 and spinetoram on megalurothrips usitatus
  • Synergistic prevention and treatment of beauveria bassiana SB038 and spinetoram on megalurothrips usitatus

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025]The screening of embodiment 1 hypervirulence strain

[0026] 1.1 Insects to be tested

[0027] Ordinary Thrips was collected from the cowpea field in Zhucun, Zengcheng District, Guangzhou City, Guangdong Province in 2017. After harvesting, it was reared with cowpea pods in the RXZ-500C intelligent artificial climate box (Ningbo Jiangnan Instrument Factory). The feeding conditions were temperature (26 ±5)°C, photoperiod 12L:12D, relative humidity (70±5)%.

[0028] 1.2 Tested strains

[0029] The strains listed for screening all belong to Beauveria bassiana bacteria, and are all preserved in the Engineering Research Center of the Ministry of Education for Biological Control, South China Agricultural University, as follows: SP433, SB036, SB062, SB037, SB015, SB063, SB051, SB041, SB004, SB043, SP016, SB038, SB050, SB006.

[0030] Of these, deposits of biological materials for patent procedures were also made for SB063 and SB038. Among them, the content of the invention r...

Embodiment 2

[0052] Identification of embodiment 2 bacterial strain SB038

[0053] (1) Collection of strain SB038

[0054] Strain SB038 was originally isolated from the soil of Zhengxiangbaiqi, Xilinguole League, Inner Mongolia Autonomous Region in 2009, and is a native strain of China.

[0055] Collect soil samples, take about 100g of soil at 10-15cm below the topsoil, pack it in a plastic bag and bring it back to the laboratory for processing. Sieve the soil sample to remove stones and sundries, then take 10g of pure soil and suspend in 90mL 0.1% Tween-80 solution, shake well, let it stand for 15min, take 2mL of its supernatant and dilute it in 8mL 0.05% Tween-80 , prepared as a soil suspension. Inoculate 0.1mL soil suspension on Bengal red agar medium plate, push the suspension evenly under the surface of the plate with a triangular glass scraper, incubate at 25°C for 3-7 days, then cut out a single colony with an inoculation loop, and inoculate it on a PDA plate The culture was cont...

Embodiment 3

[0077] Example 3 The toxicity measurement of different pesticides to common thrips

[0078] Use the drug film method to prepare pesticides with different concentration gradients. Soak a 5cm centrifuge tube (punched) in the drug solution for 2 hours, soak 1cm beans (with no holes at both ends) in the drug solution for 10 seconds, dry naturally, and then pick Put 20 adult female thrips larvae into the centrifuge tubes with beans, set 4 replicates for each concentration and raise them in an incubator, observe them for 2 days, and record their mortality.

[0079] SPSS19.0 software was used to process and analyze the experimental data, and one-way analysis of variance was used to analyze the toxicity of biological / chemical pesticides to thrips, and the Tukey test was used to test the significance of the difference to screen highly toxic pesticides.

[0080] The results showed that the corrected death rate increased with the increase of the concentration after 5 kinds of biochemical p...

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Abstract

The beauveria bassiana SB038 and the spinetoram in an insecticide disclosed by the invention have a remarkable synergistic effect on the poisoning and killing effects of megalurothrips usitatus. Long-term infection biological research and indoor biological assay show that the combined use of beauveria bassiana and spinetoram has a good prevention and treatment effect on megalurothrips usitatus, and the insecticide has a very strong application potential in biological prevention and treatment, can reduce the use amount of chemical pesticides, has the characteristics of low toxicity and low drug resistance, does not pollute the environment, does not have residues, and can favorably delay the occurrence and development of the insecticide resistance of thrip pests.

Description

technical field [0001] The invention belongs to the technical field of biological control. It specifically relates to the biological control of common thrips control, and more specifically, relates to a synergistic control of common thrips by Beauveria bassiana and spinosad. Background technique [0002] Chemical pesticides play a huge role in pest control, and the use and output of pesticides in my country rank first in the world for a long time. However, according to statistics, the effective utilization rate of pesticides in the world is less than 30%. In my country, the area where chemical pesticides are used is more than 280 million hm2 every year, and the application amount reaches 500,000-600,000 tons. About 80% of chemical pesticides directly enter the environment (Li Liang, Zhang Zhanying, Sun Huiying, et al. Discussion on pesticide hazards and green plant protection technology [J]. Hubei Plant Protection, 2018, (4): 63-64). With the long-term and large-scale use o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/30A01N43/22A01P7/04
CPCA01N63/30A01N43/22
Inventor 吴建辉田庆恒陈月银肖卡特·阿里王兴民邱宝利
Owner SOUTH CHINA AGRI UNIV
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