The invention belongs to the technical field of environment-friendly water treatment, particularly relates to a water purifying agent for wastewater treatment of tire production enterprises and further relates to a method for treating wastewater of the tire production enterprises by adopting the water purifying agent. The water purifying agent contains an inorganic water purifying agent, a microbial water purifying agent and an enzyme preparation, wherein the inorganic water purifying agent is prepared from the ingredients in parts by weight: 5-20 parts of polyaluminum chloride, 4-16 parts of polyacrylamide, 2-15 parts of ferrous sulfate and 5-16 parts of aluminum sulfate; the microbial water purifying agent is prepared from the ingredients in parts by weight: 0.5-2.0 parts of nitrobacterium powder, 0.4-1.4 parts of paracoccus denitrificans powder, 0.2-0.8 part of thiobacterium powder and 0.3-1.2 parts of aniline degrading bacteria powder; the enzyme preparation is prepared from the ingredients in parts by weight: 0.1-1.5 parts of pectinase, 0.4-1.5 parts of cellulase, 0.1-0.8 part of dehydrogenase, 0.2-0.8 part of oxidoreductase and 0.1-0.6 part of xylanase. The water purifying agent and the method have the beneficial effects that by adopting the water purifying agent for wastewater treatment, disclosed by the invention, the water purifying effect is good, the water purifying speed is high, the safety is high, and no secondary pollution is caused; materials with adsorption capacity are used as raw materials and are assisted with microbes, so as to treat the wastewater, so that the range of use is wide, the transparency of treated water is extremely high, and recycling can be achieved.