Method for preparing microecological preparation via mixed synchronous fermentation of yeasts and lactic acid bacteria and application of method

A technology of micro-ecological preparations and mixed fermentation, applied in the direction of microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problems of increasing the risk of bacterial contamination and heavy workload, and achieve the goal of solving the risk of bacterial contamination, The effect of low cost and short fermentation time

Inactive Publication Date: 2017-11-03
天津市农业科学院
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the compound micro-ecological preparations composed of yeast and lactic acid bacteria are mixed in a certain proportion after the fermentation of a single bacterial agent separately. This method requires a lot of work, and Lactobacillus plantarum is a facultative anaerobic bacteria, so it requires special device
Yeast and lactic acid bacteria have a symbiotic effect and can be mixed and fermented. CN101805696B discloses a method for co-cultivating deodorizing yeast and lactic acid bacteria. The steps of the method are: at a temperature of 20-35°C, first culture the yeast in a fermenter with aeration and stirring 2 -8h, then add lactic acid bacteria liquid, and then add lactic acid bacteria liquid for 8-14h after culturing for 18-24h. This method increases the risk of bacterial infection by inoculating 3 times during the fermentation process. The obtained bacterial agent is used in garbage dumps, Catering places, toilet deodorization

Method used

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  • Method for preparing microecological preparation via mixed synchronous fermentation of yeasts and lactic acid bacteria and application of method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Preparation of complex probiotics by mixed fermentation of yeast and lactic acid bacteria

[0024] (a) Strain activation: Inoculate Candida utilis CGMCC2.281 into sterilized YPD medium, shake culture at 25°C for 24 hours, inoculate Lactobacillus plantarum CGMCC1.11 into sterilized medium containing 90% MRS Incubate in a glass bottle at 30°C for 30 h. The YPD medium used refers to: yeast extract 10.0g, peptone 20.0g, glucose 20.0g, distilled water 1000mL; MRS medium refers to: peptone 10.0g, beef extract 10.0g, yeast 5.0g, glucose 5.0g, Tween 80 1.0mL, dipotassium hydrogen phosphate 2.0g, sodium acetate 5.0g, triammonium citrate 2.0g, magnesium sulfate 0.58g, manganese sulfate 0.25g, distilled water 1000mL.

[0025] (b) Fermentation medium: molasses 1.5%, yeast extract 0.5%, sodium acetate 0.3%, diammonium hydrogen citrate 0.1%, tomato juice 5%, potassium dihydrogen phosphate 0.2%, manganese sulfate 0.01%, initial pH 6.0 , sterilized at 115°C for 20min.

[0026] (c) S...

Embodiment 2

[0029] Preparation of complex probiotics by mixed fermentation of yeast and lactic acid bacteria

[0030] (a) Strain activation: Inoculate Candida utilis CGMCC2.281 into sterilized YPD medium, shake culture at 30°C for 20 hours, inoculate Lactobacillus plantarum CGMCC1.11 into sterilized medium containing 90% MRS Incubate in a glass infusion bottle at 34°C for 24 hours. The YPD medium used refers to: yeast extract 10.0g, peptone 20.0g, glucose 20.0g, distilled water 1000mL; MRS medium refers to: peptone 10.0g, beef extract 10.0g, yeast 5.0g, glucose 5.0g, Tween 80 1.0mL, dipotassium hydrogen phosphate 2.0g, sodium acetate 5.0g, triammonium citrate 2.0g, magnesium sulfate 0.58g, manganese sulfate 0.25g, distilled water 1000mL.

[0031](b) Fermentation medium: molasses 2.0%, yeast extract 0.6%, sodium acetate 0.4%, diammonium hydrogen citrate 0.1%, tomato juice 5%, potassium dihydrogen phosphate 0.2%, manganese sulfate 0.02%, initial pH 6.0 , sterilized at 115°C for 20min.

...

Embodiment 3

[0035] Preparation of complex probiotics by mixed fermentation of yeast and lactic acid bacteria

[0036] (a) Strain activation: inoculate Candida utilis CGMCC2.281 into sterilized YPD medium, shake culture at 25°C for 24 hours, inoculate Lactobacillus plantarum CGMCC1.11 into sterilized medium containing 90% MRS Incubate in a glass infusion bottle at 34°C for 24 hours. The YPD medium used refers to: yeast extract 10.0g, peptone 20.0g, glucose 20.0g, distilled water 1000mL; MRS medium refers to: peptone 10.0g, beef extract 10.0g, yeast 5.0g, glucose 5.0g, Tween 80 1.0mL, dipotassium hydrogen phosphate 2.0g, sodium acetate 5.0g, triammonium citrate 2.0g, magnesium sulfate 0.58g, manganese sulfate 0.25g, distilled water 1000mL.

[0037] (b) Fermentation medium: molasses 2.5%, yeast extract 0.8%, sodium acetate 0.5%, diammonium hydrogen citrate 0.2%, tomato juice 5%, potassium dihydrogen phosphate 0.2%, manganese sulfate 0.02%, initial pH 6.0 , sterilized at 115°C for 20min.

...

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Abstract

The invention discloses a method for preparing a microecological preparation via mixture synchronous fermentation of yeasts and lactic acid bacteria and application of the method. According to the technical scheme, a culture medium is made from 2.5% of molasses, 0.8% of yeast extract, 0.2% of diammonium hydrogen citrate, 0.5% of sodium acetate, 5% of tomato juice, 0.2% of monopotassium phosphate and 0.02% of manganese sulfate, and has pH of 6.0. The method of mixed fermentation includes: inoculating a strain accounting for 1-3% by volume of the fermentation culture medium, fermenting at 25-31 DEG C, stirring at 100-150 rpm, and fermenting for 17-23 h; mixing the fermentation broth and attapulgite in a mass ratio of 1:2, and airing to obtain a composite microecological preparation. The composite microecological preparation prepared herein has high concentration of effective live bacteria, has long shelf life and has prominent water-purifying effect, fewer steps such as multi-tank fermentation and post-fermentation mixing are required in the conventional preparation process of microecological preparations, bacterial staining chances are slimmed, and the production cost is reduced as industrial leftovers are used as main fermentation materials.

Description

technical field [0001] The invention belongs to a microbial fermentation method in the technical field of bioengineering, and relates to a method for mixed fermentation of Candida utilis and Lactobacillus plantarum and the application of the prepared microecological preparation in aquaculture. Background technique [0002] With the improvement of the intensive operation mode of aquaculture industry, the decay of residual bait, the deposition of biological metabolites and biological residues have caused serious organic pollution in ponds, resulting in the proliferation of harmful algae and pathogens and the deterioration of water quality. In addition, the excessive discharge of human domestic sewage, various industrial wastewater, and agricultural return water further pollutes the water source for aquaculture and deteriorates the aquaculture environment. The abuse of antibiotics and chemicals has brought about problems such as a large number of drug residues in fish and shrim...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C02F3/34C12R1/25C12R1/72
CPCC02F3/34C02F3/347C12N1/16C12N1/20
Inventor 谢凤行张峰峰周可赵玉洁赵琼
Owner 天津市农业科学院
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