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Preparation for comprehensive treatment of cultivation water body by using multiple bacterial and manufacturing method thereof

A technology for comprehensive management of aquaculture water bodies, applied in biochemical equipment and methods, chemical instruments and methods, and methods based on microorganisms, etc. The effects of stable water color, increased reproduction speed, and improved efficiency

Inactive Publication Date: 2007-11-28
SHANGHAI CHUANGBO ECOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the strains in the technology belong to different species (chemoheterotrophy and photoautotrophy, spore-forming and non-spore-forming), the growth conditions are very different, and without special induction and domestication, it is difficult to organically produce them in natural fermentation. Combined with microcapsule granulation and freeze-drying at the same time, the inactivation rate of bacteria is high, the production cost is high, and the improvement effect is difficult to guarantee

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Step 1: Breeding of production strains

[0046] a. Strain selection: Lactobacillus plantarum was isolated from the intestinal secretions of healthy wild crucian carp using lactic acid bacteria medium, and Bacillus subtilis was isolated from the activated sludge of sewage treatment plants using nutrient agar medium, and fermented in beer Saccharomyces cerevisiae was isolated from waste using yeast selection medium, Rhodopseudomonas was isolated from photosynthetic bacteria medium in shrimp ponds with good water quality, and actinomycetes were isolated from nutrient agar medium in soil near sewage treatment plants , do strain preservation in the laboratory; The present invention adopts following strain:

[0047] Rhodopseudomonas palustris (ATCC17001) Rhodopseudomonas palustris, Lactobacillus plantarum (ATCC8014) Lactobacillus plantarum, Saccharomyces cerevisiae (IFO0203) Saccharomyces cerevisiae, Bacillus subtilis (1.398) Bacillus subtilis, Nocardia Nocardia;

[0048] b....

Embodiment 2

[0073] Step 1: Breeding of production strains

[0074] Same as Example 1

[0075] The second step: separate and compound culture of beneficial strains

[0076] a. Weigh production strains by weight ratio: Rhodopseudomonas palustris 30%, lactic acid bacteria 20%, Saccharomyces cerevisiae 18%, Bacillus subtilis 20%, Actinomycetes 12%;

[0077] b. Compound culture of lactic acid bacteria, saccharomyces, and photosynthetic bacteria: Inoculate the bacterial lawn in the eggplant bottle in a medium sterilized at 120°C for 40 minutes, stir in a fermenter for 30 minutes, and the rotation speed is 200r / min. After stirring evenly Put it into a 200L sterilized plastic barrel and let it stand for 14 days. During the standstill process, measure the Ph value every day and release the gas produced to prevent the feed barrel from bursting. Fermentation ends when Ph reaches 3.5.

[0078] c. Compound culture of Bacillus subtilis and actinomycetes: Inoculate the lawn of eggplant bottles in a cult...

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Abstract

The invention relates to a kind of microbic reagent curing breed aquatics water by multi-bacteria. It can degrade and transform organic contamination of water, prohibite the growth of harmful algae and pathogeny bacteria, and promote propagation of beneficial creature, forming good entironment for growth of aquatic animal. Environment select breed and abduction select breed are adopted in this invention, spontaneous aberration and directional breed are combined, fermentated technology of original compound breed and domestication are used to make many bacteria of improved water mixed and matched each other. What' s more, growth factor and special carrier are added for promoting the growth of microbe, guaranteeing the efficiency of improvement. This product can take the place of antibiotic in feed, producing organic farm products which are green, safe and antibiotic-free.

Description

technical field [0001] The invention relates to a preparation for the comprehensive management of aquaculture water by using multiple strains. By directly adding in the water body, each strain can effectively use the eutrophic organisms in the water body, rapidly decompose and transform various organic pollutants in the water body, and inhibit The growth of harmful algae and pathogenic bacteria promotes the reproduction of beneficial organisms in the intestinal tract of animals, forming a benign ecological environment suitable for the growth of aquatic animals. The invention belongs to the technical field of agricultural biological products. Background technique [0002] With the vigorous development of aquaculture intensification in our country, the pollution of aquaculture water environment is becoming more and more serious. Aquatic animal diseases caused by environmental deterioration or infectious diseases caused by them have increased sharply, which has become the main ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/18C02F3/34C12R1/38C12R1/225C12R1/125C12R1/04
Inventor 江瀚
Owner SHANGHAI CHUANGBO ECOLOGICAL ENG
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