Method for treating nitrogen-containing wastewater by using heterotrophic nitrification-aerobic denitrification paracoccus strain

An aerobic denitrification and heterotrophic nitrification technology, applied in the field of microorganisms, can solve the problems of decreased denitrification ability, heterotrophic nitrifying bacteria denitrification ability, and bacterial strain death, etc., and achieve high tolerance to high concentrations of organic matter. Effect

Active Publication Date: 2020-01-07
LONGYAN UNIV
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  • Application Information

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Problems solved by technology

[0005] At present, the actual denitrification ability of heterotrophic nitrifying bacteria in aquaculture wastewater cannot reach the theoretical value, which may be due to the fact that the composition of actual aquaculture wa...

Method used

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  • Method for treating nitrogen-containing wastewater by using heterotrophic nitrification-aerobic denitrification paracoccus strain
  • Method for treating nitrogen-containing wastewater by using heterotrophic nitrification-aerobic denitrification paracoccus strain
  • Method for treating nitrogen-containing wastewater by using heterotrophic nitrification-aerobic denitrification paracoccus strain

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Embodiment 1

[0097] A method for treating ammonia-nitrogen wastewater by using the paracoccus bacterial strain LJ2 seed liquid of above-mentioned heterotrophic nitrification-aerobic denitrification, comprising the following steps:

[0098] Insert the seed solution of the strain into the heterotrophic nitrification medium at 3%, culture it on a shaker at 150rpm at 30°C for 48 hours, and measure the NH of the medium every 6 hours 4 + -N, NO 3 - -N, NO 2 - -N and COD content and pH value and OD 600 , and draw the curve change graph of the above indicators.

[0099] NH 4 + -N is the only nitrogen source, and the growth and heterotrophic nitrification and denitrification performance changes after 48 hours of cultivation are as follows Figure 7 Shown: From 0 to 12 hours, the growth curve changes greatly, which is the exponential growth period of LJ2; after 12 hours, the growth curve tends to be flat, and LJ2 enters the stable growth period; at 42 hours, the growth curve reaches the peak...

Embodiment 2

[0102] In this embodiment, the content of nitrogen source ammonium sulfate is fixed, sodium succinate is used as carbon source, and the carbon-nitrogen ratio is changed by adjusting the content of carbon source. C / N was set to 8, 10, 15, 20, 25, 30, 35, 40, 50, 60, 100 and 150, and each group of experiments was set up with 3 repetitions. Other components of the heterotrophic nitrification medium were different from the culture conditions. Change, 150rpm, shaker culture at 30°C, after 24 hours of culture, measure the OD in different media 600 , NH 4 + -N and TN content.

[0103] The effect of C / N on the performance of LJ2 heterotrophic nitrification is as follows: Figure 8 Shown: C / N is in the range of 8 to 150, the NH of LJ2 4 + -N removal rate can reach more than 85%. When C / N is 35, the OD of LJ2 600 The maximum reaches 0.953, NH 4 + -N removal rate was 92.83%, TN removal rate was 80.87%. When C / N increased to 40, OD 600 down to 0.843, NH 4 + The -N removal rat...

Embodiment 3

[0106] NH 4 + -N as the only nitrogen source in the growth process of ammonia nitrogen oxidizing bacteria, NH in the substrate 4 + The concentration of -N is crucial for the growth of heterotrophic nitrifying bacteria. NH 4 + - If the N concentration is too low, it cannot meet the needs of the growth of the strain. If the concentration is too high, it will cause a toxic effect on the growth of the cells. At the same time, it will inhibit the activity of key enzymes in the nitrification reaction through the substrate, thereby affecting the nitrification of heterotrophic nitrifying bacteria. active. In this example, the effects of different substrate concentrations on the heterotrophic nitrification performance of strains were studied.

[0107] Based on the improved heterotrophic nitrification medium, the fixed C / N is 60, and the initial nitrogen concentration of the medium is set to 50mg L -1 , 100mg·L -1 , 200mg·L -1 , 300mg·L -1 , 400mg·L -1 . According to the opt...

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Abstract

The invention discloses a method for treating nitrogen-containing wastewater by using aheterotrophic nitrification-aerobic denitrification paracoccus strain, wherein the nitrogen-containing wastewatercontains organic acid sodium, and the C/N ratio is 8-150. The method comprises: inoculating a heterotrophic nitrification-aerobic denitrification paracoccus seed liquid into ammonia nitrogen wastewater, and carrying out an oscillation reaction at a temperature of 25-40 DEG C at a rotating speed of 90-180rpmso as to finish the treatment of the ammonia nitrogen wastewater, wherein the strain is paracoccus LJ2, is preserved in the Guangdong culture collection center, has the preservation number ofGDMCC NO:60338, has extremely high capacity of high-concentration organic matter tolerance, and hasexcellent capacity of ammonia nitrogen removal, nitrate nitrogen removal and nitrite nitrogen removal under high organic load, so that the biological denitrification treatment of high-concentration organic matter nitrogen-containing wastewater by using paracoccus LJ2 has huge application prospect.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a method for treating nitrogen-containing wastewater by using paracoccus strains of heterotrophic nitrification-aerobic denitrification. Background technique [0002] With the continuous development of society, people's living standards are also constantly improving. In order to meet people's needs, the aquaculture industry is also growing. However, the rapid development of aquaculture industry and high-density breeding mode lead to serious environmental pollution. Aquaculture wastewater has typical "three high characteristics", with CODcr as high as several thousand to tens of thousands mg·L -1 , NH 4 + -N up to 800~2200mg·L -1 , SS exceeds the standard by dozens of times, which is high nitrogen and high organic matter load polluted wastewater. Untreated aquaculture wastewater is rich in a large amount of inorganic nitrogen, including three substances: nitrate, nitrite and ...

Claims

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Application Information

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IPC IPC(8): C02F3/34C12N1/20C02F101/16C12R1/01
CPCC02F3/34C12N1/20C02F2101/16C02F2101/163C02F2101/166C12R2001/01C12N1/205
Inventor 孙巍夏春雨宋玉文洪维祎杨雅玲苏全榕阙才英魏清斯魏登枭林彩琴龚丽梅
Owner LONGYAN UNIV
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