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Heterotrophic nitrification-aerobic denitrification paracoccus strain, seed solution and preparation method and application thereof

A technology of aerobic denitrification and heterotrophic nitrification, applied in the field of microorganisms, can solve the problems of decreased denitrification capacity, bacterial death, unsatisfactory denitrification capacity of heterotrophic nitrifying bacteria, etc., and achieves high tolerance to high concentration of organic matter. Effect

Active Publication Date: 2022-08-09
LONGYAN UNIV
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Problems solved by technology

[0005] At present, the actual denitrification ability of heterotrophic nitrifying bacteria in aquaculture wastewater cannot reach the theoretical value, which may be due to the fact that the composition of actual aquaculture wastewater is more complex, such as the high organic matter in wastewater, which inhibits the enzyme activity in heterooxynitrifying bacteria. Even lead to the death of the strain, resulting in a decrease in its denitrification ability

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  • Heterotrophic nitrification-aerobic denitrification paracoccus strain, seed solution and preparation method and application thereof
  • Heterotrophic nitrification-aerobic denitrification paracoccus strain, seed solution and preparation method and application thereof
  • Heterotrophic nitrification-aerobic denitrification paracoccus strain, seed solution and preparation method and application thereof

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Embodiment

[0063] A heterotrophic nitrification-aerobic denitrification Paracoccus strain of the present invention is preserved in the Guangdong Provincial Microbial Culture Collection Center (abbreviation: GDMCC), and the preservation number is GDMCC NO: 60338. This Paracoccus GDMCC NO: 60338 was named Paracoccus versutus LJ2.

[0064] 1) Bacteria isolation and screening

[0065] The above-mentioned Paracoccus LJ2 of the present embodiment is mainly obtained by screening the following methods:

[0066] 100 mL of activated sludge collected from Longjin Wastewater Treatment Plant in Longyan City, Fujian Province was added to 200 mL of denitrification enrichment medium, and sterile glass beads were added to the medium. 30℃, 150rpm·min -1 Shaking on a shaker, cultured for 5 days, the above bacterial suspension was transferred to fresh DM medium at a 10% inoculum amount, and the culture was repeated twice in this way to obtain an enriched bacterial suspension.

[0067] Using the 10-fold d...

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Abstract

The present invention disclosed a kind of Portal bacteria strain with a good oxide and nitrifying nitrifying.On the day, the preparation number is GDMCC NO: 60338.The Pseudococcus LJ2 of the present invention has the ability of highly tolerated high concentration of organic matter, and also has excellent ability to decar nitrogen, nitrate nitrogen and nitrite nitrogen under high organic load conditions;N is still capable of stable decades, nitrate nitrogen, and nitrite nitrogen within the range.Therefore, Pseudococcus LJ2 can provide seedlin resources for high -concentration organic matter -containing wastewater, nitrogen dehydration treatment, and has huge application value.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a heterotrophic nitrification-aerobic denitrification Paracoccus strain, a seed solution and a preparation method and application thereof. Background technique [0002] With the continuous development of society, people's living standards are also constantly improving. In order to meet people's needs, the breeding industry is also growing. However, the rapid development of the breeding industry and the high-density breeding model have resulted in serious environmental pollution. Aquaculture wastewater has typical "three high characteristics", with CODcr as high as several thousand to tens of thousands mg·L -1 , NH 4 + -N up to 800~2200mg·L -1 , SS exceeds the standard dozens of times, is high nitrogen, high organic load pollution wastewater. Untreated aquaculture wastewater is rich in a large amount of inorganic nitrogen, including nitrate, nitrite and ammonium salt, w...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/02C02F3/34C12R1/01
CPCC12N1/20C02F3/02C02F3/34C12R2001/01C12N1/205Y02W10/10
Inventor 孙巍夏春雨宋玉文洪维祎杨雅玲苏全榕阙才英魏清斯魏登枭林彩琴龚丽梅
Owner LONGYAN UNIV
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