Heterotrophic nitrification-aerobic denitrification paracoccus versutus strain, seed liquid, and preparation method and application of seed liquid
A technology of aerobic denitrification and heterotrophic nitrification, applied in the field of microorganisms, can solve the problems of decreased denitrification ability, death of bacterial strains, and insufficient denitrification ability of heterotrophic nitrifying bacteria, and achieve high tolerance to high concentrations of organic matter Effect
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[0063] A heterotrophic nitrification-aerobic denitrification Paracoccus strain of the present invention is preserved in the Guangdong Microbial Culture Collection Center (abbreviation: GDMCC), and the preservation number is GDMCC NO: 60338. The paracoccus GDMCC NO: 60338 is named Paracoccus versutus LJ2 (Paracoccus versutus LJ2).
[0064] 1) Separation and screening of strains
[0065] The Paracoccus LJ2 of the above-mentioned present embodiment is mainly screened by the following methods:
[0066] Add 100 mL of activated sludge collected from Longjin Sewage Plant in Longyan City, Fujian Province to 200 mL of denitrification enrichment medium, and add sterile glass beads to the medium. 30℃, 150rpm·min -1 The shaker was shaken and cultured continuously for 5 days, and the above bacterial suspension was transferred to fresh DM medium with 10% inoculum amount, and this culture method was repeated twice to obtain an enriched bacterial suspension.
[0067] Using the 10-fold dilu...
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