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Heterotrophic nitrification-aerobic denitrification paracoccus versutus strain, seed liquid, and preparation method and application of seed liquid

A technology of aerobic denitrification and heterotrophic nitrification, applied in the field of microorganisms, can solve the problems of decreased denitrification ability, death of bacterial strains, and insufficient denitrification ability of heterotrophic nitrifying bacteria, and achieve high tolerance to high concentrations of organic matter Effect

Active Publication Date: 2020-01-07
LONGYAN UNIV
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  • Description
  • Claims
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Problems solved by technology

[0005] At present, the actual denitrification ability of heterotrophic nitrifying bacteria in aquaculture wastewater cannot reach the theoretical value, which may be due to the fact that the composition of actual aquaculture wastewater is more complex, such as the high organic matter in wastewater, which inhibits the enzyme activity in heterooxynitrifying bacteria. Even lead to the death of the strain, resulting in a decrease in its denitrification ability

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  • Heterotrophic nitrification-aerobic denitrification paracoccus versutus strain, seed liquid, and preparation method and application of seed liquid
  • Heterotrophic nitrification-aerobic denitrification paracoccus versutus strain, seed liquid, and preparation method and application of seed liquid
  • Heterotrophic nitrification-aerobic denitrification paracoccus versutus strain, seed liquid, and preparation method and application of seed liquid

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Embodiment

[0063] A heterotrophic nitrification-aerobic denitrification Paracoccus strain of the present invention is preserved in the Guangdong Microbial Culture Collection Center (abbreviation: GDMCC), and the preservation number is GDMCC NO: 60338. The paracoccus GDMCC NO: 60338 is named Paracoccus versutus LJ2 (Paracoccus versutus LJ2).

[0064] 1) Separation and screening of strains

[0065] The Paracoccus LJ2 of the above-mentioned present embodiment is mainly screened by the following methods:

[0066] Add 100 mL of activated sludge collected from Longjin Sewage Plant in Longyan City, Fujian Province to 200 mL of denitrification enrichment medium, and add sterile glass beads to the medium. 30℃, 150rpm·min -1 The shaker was shaken and cultured continuously for 5 days, and the above bacterial suspension was transferred to fresh DM medium with 10% inoculum amount, and this culture method was repeated twice to obtain an enriched bacterial suspension.

[0067] Using the 10-fold dilu...

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Abstract

The invention discloses heterotrophic nitrification-aerobic denitrification paracoccus versutus strain. The bacterial strain is paracoccus versutus LJ2 ( Paracoccus versutus LJ2 ), and is preserved inGuangdong Province Microorganism Strain Preservation Center, wherein the preservation time is 21 March 2018, and the preservation No. is GDMCC NO:60338. The paracoccus versutus LJ2 has high capacityfor resisting high-concentration organics, can also have good capacity for removing ammoniacal nitrogen, nitrate nitrogen and nitrite nitrogen under the condition of high organic load, and also has the capacity for stably removing ammoniacal nitrogen, nitrate nitrogen and nitrite nitrogen within the range of wide C / N ratio. The paracoccus versutus LJ2 can provide inoculum sources for biological denitrification treatment on high-concentration organic nitrogen-containing waste water, and has great application value.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a heterotrophic nitrification-aerobic denitrification paracoccus strain, a seed solution and a preparation method and application thereof. Background technique [0002] With the continuous development of society, people's living standards are also constantly improving. In order to meet people's needs, the aquaculture industry is also growing. However, the rapid development of aquaculture industry and high-density breeding mode lead to serious environmental pollution. Aquaculture wastewater has typical "three high characteristics", with CODcr as high as several thousand to tens of thousands mg·L -1 , NH 4 + -N up to 800~2200mg·L -1 , SS exceeds the standard by dozens of times, which is high nitrogen and high organic matter load polluted wastewater. Untreated aquaculture wastewater is rich in a large amount of inorganic nitrogen, including three substances: nitrate, nitrite an...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/02C02F3/34C12R1/01
CPCC12N1/20C02F3/02C02F3/34C12R2001/01C12N1/205Y02W10/10
Inventor 孙巍夏春雨宋玉文洪维祎杨雅玲苏全榕阙才英魏清斯魏登枭林彩琴龚丽梅
Owner LONGYAN UNIV
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