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Method for treating nitrogen-containing wastewater by using heterotrophic nitrification-aerobic denitrification Paracoccus strain

A technology of aerobic denitrification and heterotrophic nitrification, applied in the field of microorganisms, can solve the problems of reduced denitrification capacity, bacterial death, and unsatisfactory denitrification capacity of heterotrophic nitrifying bacteria, and achieves high tolerance to high concentrations of organic matter. Effect

Active Publication Date: 2022-01-28
LONGYAN UNIV
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  • Abstract
  • Description
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Problems solved by technology

[0005] At present, the actual denitrification ability of heterotrophic nitrifying bacteria in aquaculture wastewater cannot reach the theoretical value, which may be due to the fact that the composition of actual aquaculture wastewater is more complex, such as the high organic matter in wastewater, which inhibits the enzyme activity in heterooxynitrifying bacteria. Even lead to the death of the strain, resulting in a decrease in its denitrification ability

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  • Method for treating nitrogen-containing wastewater by using heterotrophic nitrification-aerobic denitrification Paracoccus strain
  • Method for treating nitrogen-containing wastewater by using heterotrophic nitrification-aerobic denitrification Paracoccus strain
  • Method for treating nitrogen-containing wastewater by using heterotrophic nitrification-aerobic denitrification Paracoccus strain

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Embodiment 1

[0097] A method for treating ammonia-nitrogen wastewater by using the paracoccus bacterial strain LJ2 seed liquid of above-mentioned heterotrophic nitrification-aerobic denitrification, comprising the following steps:

[0098] Insert the seed solution of the strain into the heterotrophic nitrification medium at 3%, culture it on a shaker at 150rpm at 30°C for 48 hours, and measure the NH of the medium every 6 hours 4 + -N, NO 3 - -N, NO 2 - -N and COD content and pH value and OD 600 , and draw the curve change graph of the above indicators.

[0099] NH 4 + -N is the only nitrogen source, and the growth and heterotrophic nitrification and denitrification performance changes after 48 hours of cultivation are as follows Figure 7 Shown: From 0 to 12 hours, the growth curve changes greatly, which is the exponential growth period of LJ2; after 12 hours, the growth curve tends to be flat, and LJ2 enters the stable growth period; at 42 hours, the growth curve reaches the peak...

Embodiment 2

[0102] In this embodiment, the content of nitrogen source ammonium sulfate is fixed, sodium succinate is used as carbon source, and the carbon-nitrogen ratio is changed by adjusting the content of carbon source. The C / N was set to 8, 10, 15, 20, 25, 30, 35, 40, 50, 60, 100 and 150, and each group of experiments was set up with 3 repetitions, and the other components of the heterotrophic nitrification medium were different from the culture conditions. Change, 150rpm, shaker culture at 30°C, after 24 hours of culture, measure the OD in different media 600 , NH 4 + -N and TN content.

[0103] The effect of C / N on the performance of LJ2 heterotrophic nitrification is as follows: Figure 8 Shown: C / N is in the range of 8 to 150, the NH of LJ2 4 + -N removal rate can reach more than 85%. When C / N is 35, the OD of LJ2 600 The maximum reaches 0.953, NH 4 + -N removal rate was 92.83%, TN removal rate was 80.87%. When C / N increased to 40, OD 600 down to 0.843, NH 4 + The -N...

Embodiment 3

[0106] NH 4 + -N as the only nitrogen source in the growth process of ammonia nitrogen oxidizing bacteria, NH in the substrate 4 + The concentration of -N is crucial for the growth of heterotrophic nitrifying bacteria. NH 4 + - If the N concentration is too low, it cannot meet the needs of the growth of the strain. If the concentration is too high, it will cause a toxic effect on the growth of the cells. At the same time, it will inhibit the activity of key enzymes in the nitrification reaction through the substrate, thereby affecting the nitrification of heterotrophic nitrifying bacteria. active. In this example, the effects of different substrate concentrations on the heterotrophic nitrification performance of strains were studied.

[0107] Based on the improved heterotrophic nitrification medium, the fixed C / N is 60, and the initial nitrogen concentration of the medium is set to 50mg L -1 , 100mg·L -1 , 200mg·L -1 , 300mg·L -1 , 400mg·L -1 . According to the opt...

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Abstract

The invention discloses a method for treating nitrogen-containing wastewater by utilizing paracoccus strains of heterotrophic nitrification-aerobic denitrification. The nitrogen-containing wastewater contains sodium organic acid, and the C / N ratio is 8-150. The method comprises the following steps: Heterotrophic nitrification-aerobic denitrification Paracoccus seed solution is inoculated in ammonia nitrogen wastewater, and the reaction is oscillating under the conditions of temperature 25°C-40°C and rotation speed 90rpm-180rpm to complete the treatment of ammonia nitrogen wastewater; the strain is Paracoccus LJ2, preserved in the Guangdong Microbial Culture Collection Center, the preservation number is GDMCC NO: 60338. The paracoccus LJ2 has a very high ability to tolerate high-concentration organic matter, and also has an excellent ability to remove ammonia nitrogen, nitrate nitrogen and nitrite nitrogen under high organic load conditions. Therefore, the present invention utilizes paracoccus LJ2 for high The biological denitrification treatment of nitrogen-containing wastewater with high concentration of organic matter has great application prospects.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a method for treating nitrogen-containing wastewater by using paracoccus strains of heterotrophic nitrification-aerobic denitrification. Background technique [0002] With the continuous development of society, people's living standards are also constantly improving. In order to meet people's needs, the aquaculture industry is also growing. However, the rapid development of aquaculture industry and high-density breeding mode lead to serious environmental pollution. Aquaculture wastewater has typical "three high characteristics", with CODcr as high as several thousand to tens of thousands mg·L -1 , NH 4 + -N up to 800~2200mg·L -1 , SS exceeds the standard by dozens of times, which is high nitrogen and high organic matter load polluted wastewater. Untreated aquaculture wastewater is rich in a large amount of inorganic nitrogen, including three substances: nitrate, nitrite and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/34C12N1/20C02F101/16C12R1/01
CPCC02F3/34C12N1/20C02F2101/16C02F2101/163C02F2101/166C12R2001/01C12N1/205
Inventor 孙巍夏春雨宋玉文洪维祎杨雅玲苏全榕阙才英魏清斯魏登枭林彩琴龚丽梅
Owner LONGYAN UNIV
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