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Whole blood genome DNA preservative as well as preparation method and application thereof

A genome and preservative technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of harsh preservation conditions, high cost, limited wide application, etc., achieve protection stability, reduce steps, and prolong storage. effect of time

Pending Publication Date: 2021-03-30
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, many gene preservation companies have provided preservation services for personal gene samples. Most of the existing technologies store the isolated genomic DNA samples under ultra-low temperature conditions to maintain the stability of their physical and chemical properties and biological activities for a long time. The preservation conditions are harsh. High cost limits its wide application

Method used

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  • Whole blood genome DNA preservative as well as preparation method and application thereof
  • Whole blood genome DNA preservative as well as preparation method and application thereof
  • Whole blood genome DNA preservative as well as preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0043] A whole blood genome DNA preservation agent, its formula is the following components by weight: 100 parts of anticoagulant EDTA dipotassium, 120 parts of blood cell stabilizer sucrose, 10 parts of reactive cell encapsulation agent Tetraethyl orthosilicate (TEOS), 0.2 parts of 1mol / L HCl aqueous solution as a pH regulator, 10 parts of ion concentration regulator sodium chloride and 759.8 parts of RNase-free purified water.

[0044] The above-mentioned anticoagulant, blood cell stabilizer, reactive cell encapsulation agent, pH regulator, ion concentration regulator and RNase-free purified water are mixed to obtain the whole blood genome DNA preservation agent.

[0045] A method for long-term preservation of whole blood genomic DNA at room temperature, comprising the following steps: co-incubating 1.8mL of the above-mentioned whole blood genomic DNA preservative and 0.2mL of whole blood at -80°C for 24h to obtain a genome encapsulation sample in situ, and then the genome I...

Embodiment 2

[0048] A whole blood genome DNA preservation agent, its formula is the following components by weight: 120 parts of anticoagulant heparin sodium, 200 parts of blood cell stabilizer hydroxyethyl starch, 20 parts of reactive cell encapsulating agent tetrachloride Alloy acid (HAuCl 4 ), 0.5 parts of pH regulator phosphoric acid (1mol / L phosphoric acid aqueous solution), 10 parts of ion concentration regulator sodium monohydrogen phosphate-sodium dihydrogen phosphate and 649.5 parts of RNase-free purified water.

[0049] The above-mentioned anticoagulant, blood cell stabilizer, reactive cell encapsulation agent, pH regulator, ion concentration regulator and RNase-free purified water are mixed to obtain the whole blood genome DNA preservation agent.

[0050] A method for long-term preservation of whole blood genomic DNA at room temperature, comprising the following steps: co-incubating 10 mL of the above-mentioned whole blood genomic DNA preservative and 0.1 mL of whole blood at 4°...

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Abstract

The invention discloses a whole blood genome DNA preservative as well as a preparation method and application thereof, belonging to the field of biotechnology. The whole blood genome DNA preservativecomprises the following components in parts by weight: 1 to 200 parts of an anticoagulant, 1 to 200 parts of a blood cell stabilizer, 1 to 200 parts of a reactive cell encapsulant, 0.1 to 200 parts ofa pH regulator, 0.1 to 200 parts of an ion concentration regulator and 500 to 1000 parts of RNase-free purified water. According to the whole blood genome DNA preservative, the integrity of a genomeDNA structure and the stability of physicochemical properties can be ensured for a long time at room temperature, and the loss or change of genome information is avoided; besides, the storage time andlong-distance transportation of the genome sample at room temperature can be prolonged. The genome DNA long-term normal-temperature preservation and extraction method can provide powerful guarantee for disease prevention, disease treatment, forensic criminal investigation and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a normal-temperature preservation technology of biological gene samples, in particular to a whole blood genome DNA preservation agent and its preparation method and application. Background technique [0002] Genomic DNA (genomic DNA) refers to all the DNA that makes up the biological genome. The human genome DNA contains all the genetic information of the human body and is the material basis for heredity and species continuation. It makes the offspring appear similar to the parents. Preserving human genome DNA samples means preserving all the genetic information of the human body. In recent years, with the rapid development of genome sequencing technology and the promotion of a series of favorable national policies, scientists have been able to conduct a comprehensive analysis and interpretation of the genetic information of genome DNA and the code of life. More and more studies have ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6806
CPCC12Q1/6806C12Q2527/125
Inventor 朱伟雷琪周亮
Owner SOUTH CHINA UNIV OF TECH
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