Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Lactobacillus rhamnosus YZULr026 capable of efficiently degrading purine and application of lactobacillus rhamnosus YZULr026

A technology of Lactobacillus rhamnosus and purine, which is applied to the strain of Lactobacillus rhamnosus and its application field in degrading purine, can solve the problem that the strains of purine substances are rare, etc., and achieve the ability to quickly and efficiently degrade guanine, good acid resistance, Effect of reducing guanine absorption

Active Publication Date: 2021-04-30
YANGZHOU UNIV
View PDF12 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, many strains of Lactobacillus rhamnosus have been used to prepare microecological preparations such as food starters and intestinal balance regulators. However, strains suitable for rapid degradation of purine substances in vivo and in vitro are still very rare

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lactobacillus rhamnosus YZULr026 capable of efficiently degrading purine and application of lactobacillus rhamnosus YZULr026
  • Lactobacillus rhamnosus YZULr026 capable of efficiently degrading purine and application of lactobacillus rhamnosus YZULr026
  • Lactobacillus rhamnosus YZULr026 capable of efficiently degrading purine and application of lactobacillus rhamnosus YZULr026

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Screening of high-efficiency purine-degrading strains

[0036] 50 strains isolated from Tibetan traditional fermented food (yogurt, kimchi, sweet fermented grains) were activated 3 times, inoculated with MRS medium, anaerobically cultured at 37°C for 24 hours, and 1 mL of 1×10 7 The CFU / mL bacterial culture solution was centrifuged at 8000r / min at 4°C for 10min to collect the bacterial cells. And resuspend and wash the bacteria 2-3 times with 1mL sterile ultrapure water. Add 750 μL of 20 μg / mL xanthine, hypoxanthine, and guanine reaction solution to the bacteria respectively, add ultrapure water to the control sample, vortex and mix well, incubate at 37°C and 120r / min for 2 hours, and use liquid chromatography to determine Purine content in the sample.

[0037] The specific measurement process is as follows: sampling after cultivation, centrifugation at 8000r / min, 4°C for 10min to take the supernatant; filtering the supernatant through a 0.22μm microporous membrane, a...

Embodiment 2

[0040] Degradation characteristics of guanine in strain YZULr026

[0041] 100 μL of strain YZULr026 preservation solution (logarithmic phase) was inoculated into 5 mL of MRS liquid medium, cultured anaerobically at 37 °C for 24 h, and passed three times in a row. The culture solution of the activated strain was inoculated in fresh MRS liquid medium with an inoculum size of 2% by volume, and cultured anaerobically until the stationary phase. Adjust the concentration of YZULr026 bacterial solution to 10 7 CFU / mL Take 1mL in a centrifuge tube, centrifuge at 8000r / min, 4°C for 10min, collect the bacteria, wash 2-3 times with ultrapure water. (1) Add 750 μL of 20 μg / mL purine reaction solution to the cells, vortex and mix well, incubate at 37°C and 120 r / min for 5 h, and take samples for analysis at 0, 1, 2, 3, 4, and 5 h; (2) Add purine reaction solutions with concentrations of 10, 20, 50, 100, and 200 μg / mL to the bacteria, vortex and mix; incubate at 37°C for 2 hours, and take...

Embodiment 3

[0044] Physiological and biochemical identification of strain YZULr026

[0045] The obtained strain YZULr026 was inoculated with MRS solid medium by streaking, anaerobically cultured at 37°C for 24 hours, picked a single colony smear on the plate, fixed, added crystal violet staining solution dropwise, stained for 1min, washed with water; added iodine solution dropwise, React for 1 min, wash with water; add 95% ethanol dropwise to decolorize for 15-20 s, wash with water; counterstain with safranin for 1 min, wash with water; examine under microscope and record the cell morphology of the strain. At the same time, single colonies were picked for catalase test, indole, hydrogen sulfide and ammonia, nitrate reduction, arginine hydrolysis, and sugar fermentation tests.

[0046] Implementation results show that the strain is a Gram-positive bacillus ( image 3 ), catalase negative, does not produce indole, hydrogen sulfide and ammonia, does not reduce nitrate, does not hydrolyze ar...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses lactobacillus rhamnosus YZULr026 capable of efficiently degrading purine and an application of the lactobacillus rhamnosus YZULr026, the lactobacillus rhamnosus YZULr026 is preserved in China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC NO: M 2020359, and the preservation date is July 27th, 2020. The lactobacillus rhamnosus YZULr026 disclosed by the invention has good acid resistance and bile salt resistance; has efficient purine degradation capacity, and the guanine degradation rate reaches up to 87.85% after the strain and guanine are incubated in a 37 DEG C pure water system for 2 hours; YZULr026 has a unique genome structure, the highest homology of a gene sequence Gene264 for encoding alpha galactosidase and a published sequence is 93.38%, and 28 base differences exist; the strain YZULr026 is used for preparing a microbial preparation, and has a wide application prospect when being used for reducing the purine content in food in vitro and vivo and preventing hyperuricemia.

Description

technical field [0001] The invention relates to the field of biotechnology, and relates to a strain of Lactobacillus rhamnosus with high-efficiency degrading purine ability and its application in degrading purine. Background technique [0002] Purine is an important base that makes up nucleic acid. It participates in human metabolism in the form of purine nucleotides, and has important functions such as energy supply, metabolic regulation, and coenzyme composition. Due to the lack of urate oxidase in the human body, the purine-containing substances (guanylic acid, guanosine, guanine, etc.) in the body are finally catabolized into uric acid. Hyperuricemia (HUA). People with hyperuricemia must strictly control their diet and reduce the intake of exogenous purines. Studies have shown that purine-rich foods include high-protein foods such as meat, poultry, fish, seafood, eggs, soybeans, etc. Strictly controlling the intake of such foods will not only cause nutrient imbalances,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20A23L5/20A61K35/747A61P19/06C12R1/225
CPCC12N1/20A23L5/28A61K35/747A61P19/06A23V2400/175
Inventor 杨振泉刘慧敏郑香峰高璐饶胜其
Owner YANGZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com