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Genome DNA room-temperature preservation card as well as manufacturing method and application thereof

A genome and storage card technology, applied in the field of genomic DNA room temperature storage cards and biological gene samples at room temperature storage, can solve problems such as DNA stability decline, and achieve the effect of reducing steps, facilitating promotion, and avoiding loss or change.

Pending Publication Date: 2021-04-16
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, what the above patents protect is the naked DNA dissociated from the cell, without the protection of the cell structure, the DNA stability will decrease

Method used

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  • Genome DNA room-temperature preservation card as well as manufacturing method and application thereof
  • Genome DNA room-temperature preservation card as well as manufacturing method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] A genomic DNA storage card at room temperature, comprising an aminated qualitative filter paper with liquid absorption properties and a cell preservation liquid premix;

[0051] The above-mentioned aminated qualitative filter paper with liquid absorption performance is a qualitative filter paper treated with aminopropyltriethoxysilane (APTES). The specific operation method is: mix the qualitative filter paper with APTES, and the dosage is 10 μL APTES / cm 2 Qualitative filter paper, and then co-incubated for 30 minutes under sealed conditions, and then dried at room temperature at 25°C for 1 hour until completely dry to obtain aminated qualitative filter paper with liquid absorption properties.

[0052] The cell preservation liquid premix has the following components in parts by weight: 120 parts of cell stabilizer sucrose, 10 parts of reactive cell encapsulation agent TEOS, 0.2 part of 1mol / L HCl aqueous solution, 10 parts of ion Concentration regulator sodium chloride a...

Embodiment 2

[0057] A genomic DNA storage card at room temperature, comprising an aminated nitrocellulose membrane with liquid absorption properties and a cell preservation liquid premix;

[0058] The above-mentioned aminated nitrocellulose membrane with liquid absorption performance is a nitrocellulose membrane treated with 3-mercapto-1-propanamine, and the specific operation method is: mixing the nitrocellulose membrane with an aqueous solution of 3-mercapto-1-propanamine, The dosage is 30 μL 3-mercapto-1-propanamine / cm 2 The nitrocellulose membrane was then co-incubated for 30 minutes under sealed conditions, and then dried at room temperature at 25° C. for 1 hour until it was completely dry to obtain an aminated nitrocellulose membrane with liquid absorption properties.

[0059] The cell preservation liquid premix has the following components in parts by weight: 120 parts of anticoagulant heparin sodium, 200 parts of cell stabilizer hydroxyethyl starch, 20 parts of reactive cell encaps...

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Abstract

The invention discloses a genome DNA room-temperature preservation card and a manufacturing method and application thereof, and belongs to the technical field of biology. The genome DNA room-temperature preservation card comprises a medium with liquid absorption performance and a cell preservation liquid premixing agent. According to the genome DNA room-temperature preservation card, the integrity of a genome DNA structure and the stability of physicochemical properties can be ensured for a long time at room temperature, and the loss or change of genome information is avoided; and the storage time of the genome sample at room temperature can be prolonged, and long-distance transportation can be realized. The long-term normal-temperature preservation and extraction method of the genome DNA can provide powerful guarantee for mutation monitoring, disease prevention and disease treatment of somatic cells.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a technology for storing biological gene samples at room temperature, in particular to a card for storing genomic DNA at room temperature, a production method and application thereof. Background technique [0002] Genomic DNA (genomic DNA) refers to all the DNA that makes up the biological genome. The human genome DNA contains all the genetic information of the human body and is the material basis for heredity and species continuation. It makes the offspring appear similar to the parents. Preserving human genome DNA samples means preserving all the genetic information of the human body. In recent years, with the rapid development of genome sequencing technology and the promotion of a series of favorable national policies, scientists have been able to conduct a comprehensive analysis and interpretation of the genetic information of genome DNA and the code of life. More and more studies...

Claims

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Application Information

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IPC IPC(8): C12N15/10
Inventor 朱伟雷琪周亮
Owner SOUTH CHINA UNIV OF TECH
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