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Method for detecting oral squamous-cell carcinoma and method for suppressing the same

A squamous cell carcinoma and detection method technology, applied in the field of cancer detection, can solve the problem that the prognosis has not been improved, and achieve the effect of inhibiting proliferation

Inactive Publication Date: 2008-12-03
FUJIFILM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In recent years, although methods for the diagnosis and treatment of oral squamous cell carcinoma have been developed, its prognosis has not improved

Method used

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  • Method for detecting oral squamous-cell carcinoma and method for suppressing the same
  • Method for detecting oral squamous-cell carcinoma and method for suppressing the same
  • Method for detecting oral squamous-cell carcinoma and method for suppressing the same

Examples

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preparation example Construction

[0044] Dry spots can be prepared, for example, by dripping endless BACDNA or the like onto a substrate using a spotter, forming a plurality of spots, and then drying the spots. As the spotter, an inkjet printer, dot matrix printer, or dual jet (registered trademark) printer can be used, and an inkjet printer is preferably used. For example, GENESHOT (manufactured by Gaishi Co., Ltd., Nagoya City, Japan) and the like can be used.

[0045] In this way, a desired DNA-immobilization substrate can be prepared by immobilizing infinite BAC DNA or the like on a substrate, particularly preferably a solid substrate.

[0046] In addition, as one of means for directly detecting the deletion of the PRTFDC 1 gene, Southern blotting can be cited. The Southern blotting method is a method of separating and fixing the genomic DNA obtained from the sample, and detecting the presence of the gene in the sample by detecting the hybridization between the genomic DNA and the PRTFDC1 gene.

[0047] ...

Embodiment 1

[0100] Example 1: Alterations in genes in oral squamous cell carcinoma

[0101] In order to detect novel gene changes in OSCC, 18 OSCC cell lines (OM-1, OM-2, TSU, ZA, NA, Ca9-22, HOC-313, HOC- 815, HSC-2, HSC-3, HSC-4, HSC-5, HSC-6, HSC-7, KON, SKN-3, HO-1-N-1, KOSC-2) prepared genomic DNA, using MCG CancerArray-800 and MGC Whole Genome Array-4500 for CGH array analysis ( figure 1 a). The genomic DNA extracted from a normal oral epithelial cell line (RT7) was used as a control to be labeled with Cy5; the genomic DNA prepared from an oral squamous cell carcinoma cell line was used as a test DNA to be labeled with Cy3. Specifically, DpnII-digested genomic DNA (0.5 μg), respectively, in 0.6 mM dATP, 0.6 mM dTTP, 0.6 mM dGTP, 0.3 mM dCTP, 0.3 mM Cy3-dCTP (oral squamous cell carcinoma cells) or 0.3 mM Cy5 - In the presence of dCTP (normal cells), the cells were labeled with the BioPrime ArrayCGH Genomic Labeling System (manufactured by Invitrogen). Cy3 and Cy5 labeled dCTP wer...

Embodiment 2

[0114] Example 2: Isolation of genes contained in the 10p12 chromosomal deletion region in oral squamous cell carcinoma

[0115]To determine the genes involved in the homozygous deletion region of chromosome 10p12 in oral squamous cell carcinoma (HSC-6), first, using genomic PCR from HSC-6 cells, the extent of the homozygous deletion region was determined. The primer sequences used in genomic PCR are shown in Table 3.

[0116] table 3

[0117] Supplementary Table 2. Primer sequences used in this study

[0118]

[0119] From the results of the CGH array and the human genome database (http: / / genome.ucsc.edu / ), it can be confirmed that the deletion region is a deletion of up to 2.95Mb ( figure 1 b. Figure 2a). Then, it was possible to confirm the presence of 7 genes in this region.

[0120] Among them, only in HSC-6 cells (1 / 18, 5.6%; Fig. 2b), can confirm the homozygous deletion of PRTFDC1 gene, c10 or f63 gene, THNSL1 gene, GPR158 gene, MYO3A gene, GAD2 gene, and ARHGAP2...

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Abstract

An object of the present invention is to provide a method for detecting the oral squamous-cell carcinoma including the vicious degree through the change of the genome structure in the oral squamous-cell carcinoma as the index. The invention uses the genome structure in the oral squamous-cell carcinoma as the index, for detecting the oral squamous-cell carcinoma including the vicious degree. In addition, in the oral squamous-cell carcinoma, through recovering the deactivated gene, the multiplication of the oral squamous-cell carcinoma can be restrained.

Description

technical field [0001] The present invention relates to a method for detecting cancer by detecting changes in genes present in a specific chromosomal region for the purpose of early diagnosis of oral squamous cell carcinoma by observing its genotype. Background technique [0002] Oral squamous-cell carcinoma (OSCC) is classified as head and neck cancer, and is a tumor that mainly occurs in oral mucosal epithelium and other parts. The incidence of oral squamous cell carcinoma in head and neck cancer is as high as about 35%, affecting 270,000 people every year in the world (Parkin, DM., et al., CA Cancer J Clin.55, 74-108, 2002) . Oral squamous cell carcinoma is most commonly found in the tongue, followed by the gums (gums). In addition, it also occurs in buccal mucosa, jaw, floor of the mouth and other oral mucosa, jawbone, and salivary glands. [0003] In recent years, although methods for the diagnosis and treatment of oral squamous cell carcinoma have been developed, it...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68A61K48/00A61K38/00A61K31/713A61K31/7088A61P35/00
CPCC12Q2600/154C12Q1/6886C12Q2600/136A61P35/00A61P43/00
Inventor 稻泽让治井本逸势铃木江美奈
Owner FUJIFILM CORP
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