A kind of attenuated mutant strain of foot-and-mouth disease virus and its preparation method and application

Abstract

The invention provides a foot-and-mouth disease virus attenuated mutant strain and its preparation method and application, belonging to the technical field of biotechnology and biological products. The attenuated mutant strain of foot-and-mouth disease virus is to mutate the Lb AUG in the parental foot-and-mouth disease virus leader protein gene to ATC or AAA. The level is reduced, the pathogenicity to suckling mice is significantly weakened, and this mutation type can be inherited stably. Based on the genome structure of the OZK / 93‑08 vaccine strain, the invention mutates the second start codon AUG in the lead protein gene to ATC or AAA, which can significantly attenuate the O-type foot-and-mouth disease virus. The method of the attenuated vaccine strain will provide a safer platform for the production of the FMD O-type inactivated vaccine antigen.

Description

technical field

[0001] The invention belongs to the technical field of biotechnology and biological products, and in particular relates to an attenuated mutant strain of foot-and-mouth disease virus and its preparation method and application. Background technique

[0002] Foot-and-mouth disease (FMD) is an acute, febrile, highly contagious animal disease caused by foot-and-mouth disease virus (FMDV). , pigs, sheep and goats etc. The disease is one of the animal infectious diseases legally reported by the World Organization for Animal Health (OIE), and my country ranks it as the first class of animal diseases. Currently, the most effective control strategies for FMD are mainly achieved through inactivated vaccination and slaughter of infected and exposed animals. After decades of immunization with inactivated foot-and-mouth disease vaccines, some countries in the European Union and South America have effectively controlled foot-and-mouth disease, and are considered the benc...

Images