Kit for simultaneously detecting multiple cat pathogens as well as detection method and application of kit

Abstract

The invention discloses a kit for simultaneously detecting multiple cat pathogens as well as a detection method and application of the kit. A specific primer group and a probe group are obtained through design, so that rapid and accurate detection and identification on feline plague viruses, feline calicivirus, feline infectious peritonitis viruses, feline toxoplasma gondii, feline herpes viruses, feline chlamydia and feline mycoplasma in one reaction are realized at the same time. The detection kit obtained on the basis of the design is high in specificity and sensitivity, the feline pathogens with the concentration of 1.7 * 10 <2> copies / mL can be effectively detected, repeated detection is not needed, the actual application accuracy is high, and the detection kit can be widely applied to epidemic situation monitoring, prevention and control of feline virus infection.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a kit for simultaneously detecting multiple cat pathogens, a detection method and an application thereof. Background technique [0002] Feline panleukopenia, also known as feline distemper and feline infectious enteritis, is an acute and highly contagious infectious disease of cats, mainly caused by feline panleukopenia virus (FPV). FPV is a member of the genus Parvovirus in the family Parvoviridae. After entering the body, FPV can survive on the kidneys for more than one year. Therefore, it will cause serious harm to cats. The clinical manifestations are mostly characterized by sudden high fever, intractable vomiting, diarrhea, dehydration, circulatory disturbance and sharp reduction of white blood cells. If the female cat is infected with FPV during pregnancy, it will cause stillbirth, miscarriage or neurological symptoms in newborn kittens, which is more...

AI Technical Summary

Problems solved by technology

For this reason, the present invention proposes a kind of test kit for simultaneously detecting multiple cat pathogens and its detection method and application, which can solve the time-consuming problem of single feline pathogen detection, and can detect feline distemper virus, feline distemper virus, Feline calicivirus, feline infectious peritonitis virus, feline toxoplasma gondii, feline herpesvirus, feline chlamydia and feline mycoplasma 7 cat pathogens, with strong specificity and high sensitivity

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