Triple vaccine for feline calicivirus infection, feline infectious rhinotracheitis and feline panleukopenia as well as preparation method and application thereof

A technology for leukopenia and feline calicivirus, applied in the field of veterinary biological products, can solve the problems that vaccines cannot protect well, affect vaccine immunity, and low antigen levels, and reduce the number of animal stress , Reduce the number of immunizations, the effect of high antigen level

Active Publication Date: 2020-09-08
郑州爱科生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the emergence of more virus variants in recent years, the vaccines on the market cannot play a protective role very well, and there is an urgent need for the production of new epidemic strain vaccines
However, the existing virus strains used to prepare vaccines have weak virulence and low antigen TCID50 values. The low antigen level will definitely affect the immune effect of the vaccine, because after inactivated vaccines are inoculated into animals, they need to rely on antigens to Stimulate the body to produce an immune response, and finally form antibodies. Insufficient antigen content directly affects the strength of the immune response, resulting in less antibody production and low levels

Method used

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  • Triple vaccine for feline calicivirus infection, feline infectious rhinotracheitis and feline panleukopenia as well as preparation method and application thereof
  • Triple vaccine for feline calicivirus infection, feline infectious rhinotracheitis and feline panleukopenia as well as preparation method and application thereof
  • Triple vaccine for feline calicivirus infection, feline infectious rhinotracheitis and feline panleukopenia as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: the acquisition of three kinds of strains

[0033] A cat kidney cell line F81S adapted to full suspension culture, named F81S, preserved in the China Center for Type Culture Collection, with the preservation number CCTCC NO: C201799, classified as: cat kidney cell suspension adapted strain F81S, and the preservation date is: On June 29, 2017, the address of the depository unit is: China. Wuhan. Wuhan University.

[0034] Suspension cell growth medium: HYC-RDMP-28 medium from HyClone Company, supplemented with 2% newborn calf serum, the cell growth medium is the nutrient solution used for F81S cell suspension strain culture in shake flasks and bioreactor full suspension culture.

[0035] Adherent cell growth medium: Gibco's DMEM medium, supplemented with 10% newborn bovine serum, the cell growth medium is the nutrient solution used for F81 cell adherent culture.

[0036] Adhesive culture virus culture maintenance medium: DMEM medium of Gibco company, added...

experiment example 2

[0099] Experimental Example 2: Safety Test of Three Kinds of Virus Strains

[0100] Superdose safety test of triple inactivated freeze-dried vaccine for feline calicivirus disease, feline infectious rhinotracheitis and feline panleukopenia of the present invention

[0101] In this experiment, 20 healthy and susceptible kittens aged 2-3 months were selected, and the titers of anti-FCV, anti-FHV and anti-FPV antibodies in cat serum were required to be less than 2. The test was divided into 4 groups, 3 groups in the immunization group, which were inoculated with different batches of vaccines (batch numbers: F-1901, F-1902 and F-1903), with 5 cats in each group and 5 cats in the control group. The immunized group was injected with 2 mL (2 doses) triple inactivated freeze-dried vaccine for feline calicivirus disease, feline infectious rhinotracheitis and feline panleukopenia in the neck. The neck muscles of the control group were inoculated with 2 mL of frozen-thawed F81S cells. ...

experiment example 3

[0106] Experimental Example 3: Efficacy Test of Three Kinds of Vaccines

[0107] Efficacy test of triple inactivated freeze-dried vaccine for feline calicivirus disease, feline infectious rhinotracheitis and feline panleukopenia of the present invention

[0108] 1. Antibody Assay

[0109] In the test, 30 healthy and susceptible kittens aged 2-3 months (required cat serum anti-FCV, anti-FHV and anti-FPV antibody titers are all less than 2), 5 cats in each test group, injected 1.0 mL of vaccine through the neck muscle , each control group had 5 cats, and each neck muscle was inoculated with 1 mL of F81S cell freeze-thaw. 14 days after inoculation, all cats were collected from jugular vein blood, separated serum, tested for feline calicivirus and feline infectious rhinotracheitis virus neutralizing antibody, and measured HI for feline panleukopenia virus. The neutralizing antibody and HI antibody of the cats in the immunized group should not be lower than 1:16, and the neutrali...

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Abstract

The invention belongs to the technical field of veterinary biological products, and particularly relates to a triple vaccine for feline calicivirus infection, feline infectious rhinotracheitis and feline panleukopenia as well as a preparation method and application thereof. The triple vaccine comprises antigen components and a freeze-drying protective agent, and the antigen components comprise a feline calicivirus BJ strain inactivated antigen, a feline infectious rhinotracheitis virus SH strain inactivated antigen and a feline panleukopenia virus SY strain inactivated antigen. The feline calicivirus antigen, the feline infectious rhinotracheitis virus antigen and the feline panleukopenia virus antigen are prepared by adopting a full-suspension technology, the triple inactivated freeze-dried vaccine for feline calicivirus, feline infectious rhinotracheitis and feline panleukopenia is prepared through the processes of inactivation, concentration, freeze-drying and the like, three diseases are avoided by one injection, the animal immunization frequency is reduced, the animal stress frequency is reduced, and the economic cost of feeders is greatly reduced.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, in particular to a triple vaccine for feline calicivirus disease, feline infectious rhinotracheitis and feline panleukopenia and its preparation method and application. Background technique [0002] Feline calicivirus (Feline calicivirus, FCV) is one of the important members of the genus Calicivirus, which can cause respiratory infectious diseases in cats and feline animals. The disease mainly affects kittens aged 6-84 days, mostly in 56-84 days old, cats under 1 year old are the most susceptible, and the younger cats have a higher mortality rate after infection. Its main clinical symptoms are rhinitis, conjunctivitis, acute oral ulcer, pneumonia, chronic gastritis and claudication. Since Fastier isolated and identified FCV for the first time in 1957, FCV has been found in Europe, America, and Asia, and has been distributed worldwide. In recent years, due to the high vari...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/295A61K39/12A61K39/245A61K39/23A61K9/19A61K47/46A61P31/14A61P31/22
CPCA61K39/12A61K9/0019A61K9/19A61K47/46A61P31/14A61P31/22A61P31/20C12N2770/16034C12N2710/16034C12N2750/14334A61K2039/552A61K2039/70A61K2039/5252
Inventor 陈雅君李少英徐树兰
Owner 郑州爱科生物科技有限公司
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