The invention provides an efficient agrobacterium transgene method of zoysia japonica. The method is characterized by comprising the following steps: 1, stripping embryos out of mature seeds of the zoysia japonica, and placing the embryos in a calli induction medium MT4 medium for three days at the minimum; 2, conducting excitation on the agrobacterium with foreign plasmid, conducting suspension on the agrobacterium by utilizing a liquid calli subculture medium MT4-S liquid medium, and obtaining agrobacterium suspension liquid; 3, placing plant tissue obtained in the step 1 into the agrobacterium suspension liquid prepared in the step 2, processing the plant tissue through a vacuum dryer for 30 minutes, and conducting shake cultivation slowly for 1 hour; 4, taking out the plant tissue, placing the plant tissue into an MT4-S solid medium containing acetosyringone, and conducting cultivation away from light for three days at the minimum, wherein the concentration of the acetosyringone is 20 mg/L. According to the method, the transgene operation process takes short time, and it takes less than 10 days; no complex operation is needed, and the method is easy to implement.