Method for detecting aflatoxin of solid sample in baijiu production process

A kind of aflatoxin and production process technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of not being able to detect multiple toxins at the same time, few types of aflatoxins, and easy false positives, etc., to achieve strong practicability, High sensitivity and reduced false positive effect

Active Publication Date: 2016-07-20
JIANGNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, there are still few studies on liquor in China. Some scholars use the ELISA method to detect the content of aflatoxin B1 in Daqu, distiller's grains, yellow water, base wine, and finished wine; ELISA detection method is prone to false positives, and the detection results It is too large, and the presence of multiple toxins cannot be detected at the same time, and the experimental results still need to be verified qualitatively and quantitatively
Some scholars also use high-performance liquid chromatography to detect the content of aflatoxin B1 in sorghum, Daqu, wine grains, yellow water, and finished wine. However, this method has problems such as long detection time and insufficient sensitivity, and the aflatoxin B1 that can be detected simultaneously There are few types of aspergillus toxins

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  • Method for detecting aflatoxin of solid sample in baijiu production process
  • Method for detecting aflatoxin of solid sample in baijiu production process
  • Method for detecting aflatoxin of solid sample in baijiu production process

Examples

Experimental program
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Effect test

Embodiment 1

[0043] Example 1: Qualitative and quantitative detection of aflatoxin in distiller's grains

[0044] Check as follows:

[0045] (1) Extraction: Add 10 g of low-temperature water to 5 g of dried distiller's grain powder (wet distiller's grains dried through a 20-mesh sieve) and mix well, then vortex and shake 15 mL of acetonitrile for 30 seconds to extract the aflatoxin;

[0046](2) Salting out: add 1gNaCl and 4gMgSO to the mixture 4 Shake for 1min, rotate at 4000rpm for 5min; reduce the water content in the mixed extract and at the same time saturate the aqueous solution to make the mixed solution stratify into an acetonitrile layer and a water layer;

[0047] (3) Purification: take the supernatant after step (2) salting out, add purifying agent (150mgPSA, 900mgMgSO 4 ) Vortex for 30s, 4000rpm for 5min;

[0048] (4) Concentration: take the purified supernatant in step (3), dry it with nitrogen, and redissolve it in 1ml of methanol: 0.1% formic acid water (1:1) solution, fil...

Embodiment 2

[0055] Embodiment 2: Research on the effectiveness of the quantitative detection method of the present invention

[0056] Accurately weigh 5 g of dried distiller's grains, add different volumes of standard mixed solutions, perform detection according to the method of Example 1, and prepare a corresponding standard curve. The specific concentrations are shown in Table 2 to Table 5.

[0057] Table 2 AFB1 standard curve concentration

[0058]

[0059] Table 3 AFB2 standard curve concentration

[0060]

[0061] Table 4 AFG1 standard curve concentration

[0062]

[0063] Table 5 AFG2 standard curve concentration

[0064]

[0065] Accurately draw each 5ul of the above-mentioned series of solution standard solutions, sample injection analysis, record the chromatographic peak area of ​​each component to be measured, take the injection concentration as the abscissa (X), and the peak area as the ordinate (Y), carry out regression analysis, the result ( See Table 6) show t...

Embodiment 3

[0078] Embodiment 3: the influence of detection condition on detection result

[0079] The present invention also compares the influence of different processing methods on the detection effect.

[0080] (1) Influence of sample state: use wet distiller's grains instead of dry crushed distiller's grains as a sample, and other steps are consistent with Example 1. The results show that the finally detected aflatoxin content is about 35% lower than that in Example 1, the stability and recovery rate of detection are seriously affected, and the effective detection of aflatoxin cannot be realized at all.

[0081] (2) The influence of the extraction solvent: Methanol is used instead of acetonitrile as the extraction solvent, and other steps are consistent with Example 1. The results showed that the finally detected aflatoxin content was about 22% lower than that in Example 1, and the stability and recovery rate of the detection were seriously affected. In addition, the inventors have...

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Abstract

The invention discloses a method for detecting aflatoxin of a solid sample in the baijiu production process, and belongs to the technical field of food detection.The method comprises the steps that after correlative pretreatment is carried out on baijiu distillers' grains, a UPLC-MS / MS detector is combined to detect existence of aflatoxin in the baijiu distillers' grains.A novel baijiu quality safety index detecting technology is provided, operation is easy and convenient, infinitesimal aflatoxin possibly existing in the baijiu distillers' grains can be fast monitored in time, and therefore the quality safety of baijiu is effectively controlled.

Description

technical field [0001] The invention relates to a method for detecting aflatoxin in solid samples in the liquor production process, belonging to the technical field of food detection. Background technique [0002] Aflatoxins belong to mycotoxins and are classified as "Class I carcinogens" by the Cancer Research Institute of the World Health Organization. It is very easy to pollute food crops, meat and dairy products, etc., and its harmfulness is that it has a destructive effect on human and animal liver tissues. In severe cases, it can cause liver cancer or even death. [0003] Grain crops are the main production raw materials of the liquor industry, and it is very likely that aflatoxin and toxin-producing microorganisms will be brought into liquor, threatening the safety of liquor production. Now there are many safety problems in the food industry, but there are almost no reports about the impact of aflatoxin on liquor production. Therefore, it is very necessary to verify...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 韩业慧徐岩
Owner JIANGNAN UNIV
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