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Method for concentrating highly effective nitrobacteria in active sludge

A technology of activated sludge method and nitrifying bacteria, applied in chemical instruments and methods, bacteria, water/sludge/sewage treatment, etc.

Active Publication Date: 2008-08-13
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The nitrifying bacteria obtained by this method are not suitable for treating wastewater with an ammonia nitrogen concentration higher than 300 mg / L and with little or no organic content

Method used

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  • Method for concentrating highly effective nitrobacteria in active sludge
  • Method for concentrating highly effective nitrobacteria in active sludge

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Nitrifying bacteria are enriched by intermittent activated sludge method. The activated sludge is taken from the outlet sludge of the secondary settling tank in the wastewater treatment workshop of the refinery, and inoculated into the enrichment reactor with an inoculation amount of 30v%, with a total volume of 3L , and then add the enrichment medium, wherein the composition of the enrichment medium used is (NH 4 ) 2 SO 4 , FeSO 4 ·7H 2 O, MgSO 4 ·7H 2 O, NaCl, CaCl 2 and KH 2 PO 4 , the concentration is as follows: NH 4+ -N initial concentration is 150mg / L, final concentration is 1000mg / L; Fe 2+ The concentration is 12mg / L; Mg 2+ The concentration is 18mg / L; Na + The concentration is 800mg / L; Ca 2+ The concentration is 16mg / L; KH 2 PO 4 The concentration is 260mg / L. During the enrichment process, NaHCO was used 3 Control pH. Culture conditions: temperature: 24°C; pH=6.0-7.5; SV: 15%-20%; DO: 10 mg·L -1 . One cycle per day, the water inlet time is 20...

Embodiment 2

[0028] The nitrifying bacteria were enriched by the batch activated sludge method. The activated sludge was taken from the sludge at the outlet of the secondary sedimentation tank in the wastewater treatment workshop of the refinery, and was inoculated into the enrichment reactor with an inoculation amount of 22v%, with a total volume of 3L. , and then add the enrichment medium, the composition of the enrichment medium used is the same as that of Example 1, but the concentration is different, as follows: NH 4 + -N initial concentration is 100mg / L, final concentration is 550mg / L; Fe 2+ The concentration is 6mg / L; Mg 2 + The concentration is 10mg / L; Na + The concentration is 300mg / L; Ca 2+ The concentration is 8mg / L; KH 2 PO 4 The concentration is 100mg / L. During the enrichment process, NaHCO was used 3 Control pH. The culture conditions are: temperature: 25°C; pH=6.0-7.5; SV: 15%-25%; DO: 5 mg·L -1 . 2 cycles per day, the water inlet time is 10 minutes, the aeration ...

Embodiment 3

[0031] The nitrifying bacteria were enriched by the batch activated sludge method. The activated sludge was taken from the outlet sludge of the catalyst wastewater treatment workshop, and was inoculated into the enrichment reactor with an inoculum of 28v%, with a total volume of 3L, and then the enrichment reactor was added. The enriched culture solution used is the same as Example 1, but the concentration is different, as follows: NH 4 + -N initial concentration is 180mg / L, final concentration is 800mg / L; Fe 2+ The concentration is 10mg / L; Mg 2+ The concentration is 12mg / L; Na + The concentration is 500mg / L; Ca 2+ The concentration is 10mg / L; KH 2 PO 4 The concentration is 200mg / L. During the enrichment process, NaHCO was used 3 Control pH. The culture conditions are: temperature: 28°C; pH=6.0-8.5; SV: 15%-20%; DO: 8 mg·L -1 . One cycle per day, the water inlet time is 30 minutes, the aeration time is 22.5 hours, the natural sedimentation is 40 minutes, and the supe...

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Abstract

The invention discloses a enrichment method of nitrifying organism. The method adapts discontinuous activated sludge process by gradually improving ammonia nitrogen ph indicator in nutrient fluid to enrich. The main component of the enrichment nutrient fluid is inorganic salt comprising microelement Fe, Mg, Na, K and amortization liquor, in which inceptive concentration of NH(4)(+-N) is 100mg / L to 200mg / L, final concentration is 500mg / L to 1200mg / L and COD is less than or equal to 200mg / L. The method can restrain evidence the growth of sundry bacterium such as carbonizing bacterium, is propitious for nitrifying organism to become ascendency bacterium and resist more and more high ammonia nitrogen concentration, and finally disposing concentration of the ammonia nitrogen wasted water is up to 1200mg / L and high concentration ammonia nitrogen in wasted water can be reduce to less 10mg / L.

Description

technical field [0001] The invention relates to a high-efficiency nitrifying bacteria enrichment method using activated sludge as a raw material, and belongs to the field of biotechnology. Background technique [0002] In recent years, with the development of industry and agriculture, the problem of eutrophication of water bodies has become increasingly prominent, and the impact of ammonia nitrogen wastewater on the environment has attracted great attention in the field of environmental protection worldwide. [0003] With the increasing requirements of countries around the world to meet the discharge standards for external wastewater discharge and the continuous strengthening of environmental protection law enforcement, it is more difficult to treat wastewater with high ammonia nitrogen content. Sewage treatment plants in various countries are trying to find a technically applicable and economically feasible treatment method for ammonia nitrogen wastewater, especially some c...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/12
CPCY02W10/10
Inventor 高会杰张全许谦黎元生佟明友
Owner CHINA PETROLEUM & CHEM CORP
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