Automatic negative pressure control method for microfiltration membrane slice preparation and dyeing detection

Abstract

The invention discloses an automatic negative pressure control method for microfiltration membrane slice preparation and dyeing detection. According to the automatic negative pressure control method for microfiltration membrane slice preparation and dyeing detection, a negative pressure forming system and a negative pressure distribution system are included; the negative pressure forming system comprises a gas negative pressure pump and a surge flask connected with the gas negative pressure pump, a waste liquor flask is connected to the front end of the surge flask, and a negative pressure detection controller is arranged on a pipeline between the surge flask and the waste liquor flask; the negative pressure distribution system comprises a negative pressure distribution device and multiple pinch valves arranged on the negative pressure distribution device, and a negative pressure pipe is connected to each pinch valve and connected with the bottom of a filter type sandwich cup; the negative pressure forming system is communicated with the negative pressure distribution device; the top ends of the filter type sandwich cups are used for adding liquid to be detected. For the automatic negative pressure control method for microfiltration membrane slice preparation and dyeing detection, specimen filling, pumping, dyeing, cleaning and other processes are completed automatically in a fully automatic mode, it is guaranteed that the negative pressure and gas flow of the pipeline are constant, and it is guaranteed that when multiple specimens work simultaneously, the basically consistent state is kept.

Description

technical field [0001] The invention relates to a method of using membrane separation technology for inspection and detection in the fields of medicine, biology, environmental science, etc., to perform film production and dyeing, specifically an automatic negative pressure control for microporous membrane film production and dyeing detection method. Background technique [0002] It is a new type of morphological detection technology to use microporous filter membrane to collect tangible substances in specimens and directly make the filter membrane transparent and observe it under a microscope, which can be widely used in medical examination and other fields to detect tiny tangible substances. Due to the softness and fragility of the microporous filter membrane, it is limited in one aspect, and the methods used are basically directly pasted on the bottom of the interlayer cup or mechanically fixed by the interlayer cup. [0003] For example, Chinese Patent Application No. 20...

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Problems solved by technology

[0004] Although the above method can be used very well, it requires a complicated operation process of removing the microporous membrane. Since the microporous membrane is relatively light, it is necessary to re-apply the microporous membrane on the slide when observing under the microscope. Fixation (covered with a cover glass), and the removal and placement of the filter cup

Therefore, it is more difficult to operate with automated equipment

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