Method for preparing tissue slices

Abstract

The invention discloses a method for preparing tissue slices. The method comprises the following steps: 1) fixing a tissue block: fixing the tissue block in a fixing agent; 2) dehydrating: sequentially arranging the tissue block in dioxane aqueous solutions from a lower concentration to a higher concentration for dehydrating; 3) paraffin infiltration coating: completely immersing the dehydrated tissue block into molten paraffin and coating the tissue block by the paraffin; 4) slicing and drying: drying the tissue block, adding the slices into warm water, enabling the slices to be attached into a glass slide coated with a tissue slice adhesive after the slices are fully expanded, and drying; 5) rehydrating: sequentially arranging the tissue block in dioxane aqueous solutions from a higher concentration to a lower concentration for hydrating; 6) dyeing: dyeing the slices by using a dyeing agent; 7) transparentizing, namely immersing the dyed slices into xylene to deparaffinate the slices; and 8) storing. According to the method disclosed by the invention, the slice preparation step is reduced, the slice preparation time is shortened, and tissue block contraction is avoided due to dioxane, so that the quality of the slices is high.

Description

technical field

[0001] The invention relates to a method for preparing slices, in particular to a method for preparing tissue slices. Background technique

[0002] Paraffin section is the most widely used method in routine histological preparation techniques. This method can not only be used to observe the morphological structure of normal cells and tissues, but also be used to study, observe and judge the morphological changes of cells and tissues in pathology and forensic science. In teaching, most of the section samples observed under light microscope are prepared by paraffin section method. Paraffin sections have also been widely used in research in many disciplines.

[0003] The living cells or tissues of organisms are mostly colorless and transparent, and there is a lack of contrast between various tissues and various structures in cells. The tissue is corrupted and loses its original normal structure. Therefore, the tissue can only be clearly identified after being ...

Examples

Embodiment 1

[0036] The invention discloses a method for preparing tissue slices, comprising the following steps:

[0037] 1) Fix the tissue block: fix the tissue block in a fixative; the fixative is Born's solution; the volume of the fixative is 15 times the volume of the tissue block; the fixation time is 8 hours; the fixation temperature is lower than the ambient temperature;

[0038] 2) Dehydration: Dehydrate the tissue pieces in successively dehydrated dioxane aqueous solutions from low to high concentrations; the concentrations of the dioxane aqueous solutions from low to high are 30wt%, 50wt%, 70wt%, 90wt % and 100wt%; the dehydration time in the 30wt% dioxane aqueous solution is 30min; the dehydration time is 30min in the 50wt% dioxane aqueous solution; the 70wt% dioxane aqueous solution The dehydration time in medium is 30min; the dehydration time in 90wt% dioxane aqueous solution is 30min; the dehydration time in 100wt% dioxane aqueous solution is 15min;

[0039] 3) Wrapping thr...

Embodiment 2

[0047]The invention discloses a method for preparing tissue slices, comprising the following steps:

[0048] 1) Fix the tissue block: fix the tissue block in a fixative; the fixative is 4wt% paraformaldehyde solution; the volume of the fixative is 18 times the volume of the tissue block; the fixation time is 24h; the fixation temperature is lower than the environment temperature;

[0049] 2) Dehydration: Dehydrate the tissue pieces in successively dehydrated dioxane aqueous solutions from low to high concentrations; the concentrations of the dioxane aqueous solutions from low to high are 30wt%, 50wt%, 70wt%, 90wt % and 100wt%; in the concentration of 30wt% dioxane aqueous solution, the dehydration time is 30min; in the concentration of 50wt% dioxane aqueous solution, the dehydration time is 25min; in the concentration of 70wt% dioxane aqueous solution The dehydration time in medium is 25min; the dehydration time in 90wt% dioxane aqueous solution is 25min; the dehydration time...

Embodiment 3

[0057] The invention discloses a method for preparing tissue slices, comprising the following steps:

[0058] 1) Fix the tissue block: fix the tissue block in a fixative; the fixative is 40wt% formaldehyde solution; the volume of the fixative is 12 times the volume of the tissue block; the fixation time is 10 h; the fixation temperature is lower than the ambient temperature;

[0059] 2) Dehydration: Dehydrate the tissue pieces in successively dehydrated dioxane aqueous solutions from low to high concentrations; the concentrations of the dioxane aqueous solutions from low to high are 30wt%, 50wt%, 70wt%, 90wt % and 100wt%; in the concentration of 30wt% dioxane aqueous solution, the dehydration time is 35min; in the concentration of 50wt% dioxane aqueous solution, the dehydration time is 35min; in the concentration of 70wt% dioxane aqueous solution The dehydration time in medium is 35min; the dehydration time in 90wt% dioxane aqueous solution is 35min; the dehydration time in 10...

technical field

[0001] The invention relates to a method for preparing slices, in particular to a method for preparing tissue slices. Background technique

[0002] Paraffin section is the most widely used method in routine histological preparation techniques. This method can not only be used to observe the morphological structure of normal cells and tissues, but also be used to study, observe and judge the morphological changes of cells and tissues in pathology and forensic science. In teaching, most of the section samples observed under light microscope are prepared by paraffin section method. Paraffin sections have also been widely used in research in many disciplines.

[0003] The living cells or tissues of organisms are mostly colorless and transparent, and there is a lack of contrast between various tissues and various structures in cells. The tissue is corrupted and loses its original normal structure. Therefore, the tissue can only be clearly identified after being ...

Examples

Embodiment 1

[0036] The invention discloses a method for preparing tissue slices, comprising the following steps:

[0037] 1) Fix the tissue block: fix the tissue block in a fixative; the fixative is Born's solution; the volume of the fixative is 15 times the volume of the tissue block; the fixation time is 8 hours; the fixation temperature is lower than the ambient temperature;

[0038] 2) Dehydration: Dehydrate the tissue pieces in successively dehydrated dioxane aqueous solutions from low to high concentrations; the concentrations of the dioxane aqueous solutions from low to high are 30wt%, 50wt%, 70wt%, 90wt % and 100wt%; the dehydration time in the 30wt% dioxane aqueous solution is 30min; the dehydration time is 30min in the 50wt% dioxane aqueous solution; the 70wt% dioxane aqueous solution The dehydration time in medium is 30min; the dehydration time in 90wt% dioxane aqueous solution is 30min; the dehydration time in 100wt% dioxane aqueous solution is 15min;

[0039] 3) Wrapping thr...

Embodiment 2

[0047]The invention discloses a method for preparing tissue slices, comprising the following steps:

[0048] 1) Fix the tissue block: fix the tissue block in a fixative; the fixative is 4wt% paraformaldehyde solution; the volume of the fixative is 18 times the volume of the tissue block; the fixation time is 24h; the fixation temperature is lower than the environment temperature;

[0049] 2) Dehydration: Dehydrate the tissue pieces in successively dehydrated dioxane aqueous solutions from low to high concentrations; the concentrations of the dioxane aqueous solutions from low to high are 30wt%, 50wt%, 70wt%, 90wt % and 100wt%; in the concentration of 30wt% dioxane aqueous solution, the dehydration time is 30min; in the concentration of 50wt% dioxane aqueous solution, the dehydration time is 25min; in the concentration of 70wt% dioxane aqueous solution The dehydration time in medium is 25min; the dehydration time in 90wt% dioxane aqueous solution is 25min; the dehydration time...

Embodiment 3

[0057] The invention discloses a method for preparing tissue slices, comprising the following steps:

[0058] 1) Fix the tissue block: fix the tissue block in a fixative; the fixative is 40wt% formaldehyde solution; the volume of the fixative is 12 times the volume of the tissue block; the fixation time is 10 h; the fixation temperature is lower than the ambient temperature;

[0059] 2) Dehydration: Dehydrate the tissue pieces in successively dehydrated dioxane aqueous solutions from low to high concentrations; the concentrations of the dioxane aqueous solutions from low to high are 30wt%, 50wt%, 70wt%, 90wt % and 100wt%; in the concentration of 30wt% dioxane aqueous solution, the dehydration time is 35min; in the concentration of 50wt% dioxane aqueous solution, the dehydration time is 35min; in the concentration of 70wt% dioxane aqueous solution The dehydration time in medium is 35min; the dehydration time in 90wt% dioxane aqueous solution is 35min; the dehydration time in 10...

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