Method for detecting genetic toxicity of organic pollutants in water

A technology for organic pollutants and genotoxicity, which is applied in biochemical equipment and methods, and the determination/inspection of microorganisms. Effect

Inactive Publication Date: 2013-09-04
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Claims
  • Application Information

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Problems solved by technology

The test method for the detection of the genetic toxicity of organic pollutants in the water body to the peripheral blood red blood cells of the rare gobi carp has not yet been carried out.

Method used

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  • Method for detecting genetic toxicity of organic pollutants in water
  • Method for detecting genetic toxicity of organic pollutants in water
  • Method for detecting genetic toxicity of organic pollutants in water

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1, the detection of dimethyl sulfoxide genotoxicity

[0040] (1) Detection method and steps

[0041] (1) Domestication of rare goner crucian carp: domesticate the same batch of rare goner carp with similar body length and weight for 14 days, during which there is no natural dead fish phenomenon, and the micronucleus rate in peripheral blood reaches about 0.1‰, which can be used for experiments;

[0042] (2) The pre-test selects the appropriate concentration range of dimethyl sulfoxide for treatment, and there is no dead fish at the highest exposure concentration to be adopted;

[0043] (3) Preparation of exposure treatment solution: Dissolve dimethyl sulfoxide in activated carbon to treat tap water, and prepare an aqueous solution with a volume fraction of 0.03%, which is the prepared exposure treatment solution;

[0044] (4) Exposure treatment: a blank control without adding dimethyl sulfoxide was set; the experimental exposure group randomly selected 5 rar...

Embodiment 2

[0047] Embodiment 2, the detection of benzo [a] pyrene genotoxicity

[0048] (1) Detection method and steps

[0049] (1) Domestication of rare goner crucian carp: domesticate the same batch of rare goner carp with similar body length and weight for 14 days, during which there is no natural dead fish phenomenon, and the micronucleus rate in peripheral blood reaches about 0.1‰, which can be used for experiments;

[0050] (2) Select the appropriate concentration range for the pre-test; no dead fish were observed at the highest exposure concentration to be used, indicating that it can be used in the subsequent micronucleus exposure test.

[0051] (3) Preparation of exposure treatment solution: Dissolve benzo[a]pyrene in dimethyl sulfoxide, and prepare concentrations of 0mg / L, 4 / 3mg / L, 20 / 3mg / L, 100 / 3mg / L L, 500 / 3mg / L mother liquor, then take the same volume of mother liquor and dissolve them in tap water filtered by activated carbon, and prepare benzo[a]pyrene concentrations of 0...

Embodiment 3

[0064] The detection of the genotoxicity of embodiment 3, 4-nitroquinoline-1-oxide (4-NQO)

[0065] (1) Detection method and steps

[0066] (1) Domestication of rare goner crucian carp: domesticate the same batch of rare goner carp with similar body length and weight for 14 days, during which there is no natural dead fish phenomenon, and the micronucleus rate in peripheral blood reaches about 0.1‰, which can be used for experiments;

[0067] (2) The appropriate concentration range was selected for the pre-test; no dead fish were observed in the exposure treatment with the highest exposure concentration proposed, indicating that it can be used in subsequent micronucleus exposure tests;

[0068] (3) Preparation of exposure treatment solution: 4-nitroquinoline-1-oxide (4-NQO) was dissolved in dimethyl sulfoxide, and the concentrations were prepared to be 0mg / L, 1 / 3mg / L, 5 / 3mg / L, 25 / 3mg / L, 125 / 3mg / L mother liquor, and then take the same volume of mother liquor and dissolve them i...

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Abstract

The invention provides a method for detecting the genetic toxicity of organic pollutants. The method comprises the following steps of: (1) treating gobiocypris rarus by a water solution of an organic-pollutant-containing sample to be detected in an exposed way; and (2) detecting the peripheral blood erythrocyte of the gobiocypris rarus which is treated in the exposed way, carrying out statistics on cell micronucleus rate and drawing a dosage-effect curve. The detection method is sensitive, simple, convenient, rapid, more visual and accurate, has good result repeatability, and has important significance for the research of genetic toxicity of water quality.

Description

technical field [0001] The invention belongs to the field of water environment research and relates to a method for detecting genetic toxicity of organic pollutants. Background technique [0002] Increased environmental pollution poses a threat to people's health. Various research methods have been carried out to evaluate genotoxicity. Since the micronucleus test was first proposed by Heddle et al., the micronucleus test has been more and more widely used in chromosome / spindle damage due to its advantages of economy, simplicity, and high reliability. Effect detection. [0003] Genotoxic substances in the water environment can induce micronuclei in fish. By monitoring the change of micronucleus rate in fish erythrocytes, we can study the genotoxicity of exogenous substances and evaluate the environmental water quality. At present, researchers have discussed the effectiveness of the red blood cell micronucleus test for various fish. Domestic scholars have successfully adopte...

Claims

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Application Information

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IPC IPC(8): C12Q1/02
Inventor 王子健刘楠楠马梅査金苗许宜平饶凯锋
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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