Rare gobiocypris rarus embryotoxicity determination method and water body monitoring and early warning method

Abstract

The invention belongs to the technical field of toxicity determination, and discloses a gobiocypris rarus embryotoxicity determination method and a water body monitoring and early warning method. Thegobiocypris rarus embryotoxicity determination method comprises the following steps: taking a pesticide stock solution, and gradually diluting the pesticide stock solution with standard dilution wateraccording to an equal ratio differential to obtain required concentrations; adopting a cell culture plate as an exposure container, and adding fertilized eggs into each hole; with dPS statistical analysis software, carrying out statistical analysis on the gobiocypris rarus embryo death rate data through a probability value analysis method, and calculating LC50 and 95% confidence limit of LC50; respectively taking 96-LC50 values of single exposure of the two pesticides to the gobiocypris rarus embryos as a toxicity unit, and respectively solving the LC50 values and 95% confidence limits of thetwo pesticides in a mixed system by adopting DPS software after the two pesticides are contaminated for 96 hours; and evaluating the influence of the composite exposure of triazophos and fenvalerateon the embryotoxicity of the gobiocypris rarus according to a Marking addition exponential method. The invention provides an important scientific basis for monitoring, early warning, restoration and treatment of pesticide composite exposure in water.

Description

technical field [0001] The invention belongs to the technical field of toxicity determination, and in particular relates to a toxicity determination method of rare goner crucian carp embryos and a water body monitoring and early warning method. Background technique [0002] Triazophos is a poisonous and broad-spectrum organophosphorus insecticide and acaricide, which has strong contact and stomach poisoning effects, and is mainly used for pest control of fruit trees, cotton, and cereal crops. Since the late 1980s, coastal areas such as Zhejiang and Fujian in my country have begun to use a large amount of triazophos as a cleaning agent for cleaning coatings and ponds in seawater shellfish farming. Due to the extensive use of triazophos in agriculture, forestry, and fishery production, it enters water bodies through surface runoff and harms aquatic organisms, resulting in large fishery economic losses. The pollution of triazophos pesticides to estuaries, bays and aquaculture ...

AI Technical Summary

Problems solved by technology

[0005] Through the above analysis, the existing problems and defects of the existing technology are: the toxicity of pesticides to the rare gobi carp is currently limited to the evaluation of a single pesticide, and the environmental effects of the interaction between pesticides cannot be explained by the mechanism of action of a single compound, and it is difficult to truly respond real environment quality

[0006] The difficulty in solving the above problems and defects is: due to the adsorption and accumulation of different pesticides in water, the difference in chemical properties and complex interactions, the previous physical and chemical analysis methods can detect the concentration of pesticides in water, but cannot determine the concentration of pesticides. pollution effect

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