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Triazophos biosensor based on two-dimensional nano material

A technology of two-dimensional nanomaterials and biosensors, which is applied in the fields of analyzing materials, electrochemical variables of materials, and material analysis through electromagnetic means. The effect of promotion value, fast response speed, and simple detection method

Active Publication Date: 2021-02-09
SUZHOU CHIEN SHIUNG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on how to firmly modify acetylcholinesterase on the electrode, and there have been no reports on the co-modification of acetylcholinesterase with two-dimensional nanomaterials such as graphite phase carbon nitride.

Method used

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  • Triazophos biosensor based on two-dimensional nano material
  • Triazophos biosensor based on two-dimensional nano material
  • Triazophos biosensor based on two-dimensional nano material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] (1): Refer to the literature (Acta Catalytica Sinica, 2015, 36(12): 2089-2094) to obtain graphitic carbon nitride by high-temperature nitriding thermal polymerization, such as figure 1 As shown, it is the Fourier transform infrared spectrogram of the prepared graphite phase carbon nitride; weigh the graphite phase carbon nitride, add a certain amount of water, and ultrasonically obtain the suspension with a final concentration of graphite phase carbon nitride of 100mg / mL solution, followed by centrifugation to remove large particulate matter.

[0046] (2): Weigh 121.1 g of tris (Tris) and dilute to 1 L to obtain a 1M Tris–HCl stock solution. Take 50mL of 1M Tris-HCl solution, add 0.2g of bovine serum albumin (BSA), add 800mL of double distilled water, adjust the pH to 8.0, and adjust the volume to 1L to obtain a 50mM Tris-HCl buffer solution containing 0.2g (BSA) ( pH 8.0).

[0047] (3): Weigh 2 mg of acetylcholinesterase (500 U / mg), dissolve it with the above 50 mM T...

Embodiment 2

[0052] (1): Refer to the literature (Acta Catalytica Sinica, 2015, 36(12): 2089-2094) to obtain graphite phase carbon nitride by high-temperature nitriding thermal polymerization; weigh the graphite phase carbon nitride, and add a certain amount of water , sonicated to obtain a graphite-phase carbon nitride suspension with a final concentration of 50 mg / mL, which was then centrifuged to remove large particles.

[0053] (2): Weigh 121.1 g of tris (Tris) and dilute to 1 L to obtain a 1M Tris–HCl stock solution. Take 50mL of 1M Tris–HCl solution, add 0.1g of bovine serum albumin (BSA), add 800mL of double distilled water, adjust the pH to 8.0, and adjust the volume to 1L to obtain a 50mM Tris–HCl buffer solution containing 0.1g (BSA) ( pH 8.0).

[0054] (3): Weigh 0.5 mg of acetylcholinesterase (500 U / mg), dissolve it with the above-mentioned 50 mM Tris-HCl buffer solution and dilute to 250 mL to obtain a 1 U / mL acetylcholine solution.

[0055] (4): Take 5 μL of the above graph...

Embodiment 3

[0059] (1): Refer to the literature (Acta Catalytica Sinica, 2015, 36(12): 2089-2094) to obtain graphite phase carbon nitride by high temperature nitriding thermal polymerization; weigh the graphite phase carbon nitride, add a certain amount of water, Sonicate to obtain a graphite phase carbon nitride suspension with a final concentration of 150 mg / mL, which is then centrifuged to remove large particles.

[0060] (2): Weigh 121.1 g of tris (Tris) and dilute to 1 L to obtain a 1M Tris–HCl stock solution. Take 50mL of 1M Tris-HCl solution, add 0.3g of bovine serum albumin (BSA), add 800mL of double distilled water, adjust the pH to 8.0, and dilute to 1L to obtain a 50mM Tris-HCl buffer solution containing 0.3g (BSA) ( pH 8.0).

[0061] (3): Weigh 3 mg of acetylcholinesterase (500 U / mg), dissolve it with the above 50 mM Tris–HCl buffer solution and dilute to 250 mL to obtain a 6 U / mL acetylcholine solution.

[0062] (4): Take 15 μL of the above graphitic carbon nitride solution...

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Abstract

Triazophos serves as a substitute of methamidophos, is a broad-spectrum organic pesticide and is widely used for preventing and treating insect pests. However, triazophos has strong stomach toxicity and contact toxicity, and residues of triazophos have important influence on the environment and human health. The acetylcholin esterase is fixed on the electrode by adopting a two-dimensional nano material co-modification method, so that the detection sensitivity is improved, and the quantitative detection of the acetylcholin esterase inhibitor triazophos is realized.

Description

technical field [0001] The invention belongs to the field of organic phosphorus detection, in particular to a triazophos biosensor based on two-dimensional nanometer materials. Background technique [0002] As a substitute for methamidophos, triazophos is a broad-spectrum organic pesticide widely used in pest control. However, triazophos has strong stomach toxicity and contact toxicity, and its residues have important impacts on the environment and human health. Traditional methods include gas chromatography and high performance liquid chromatography to analyze and test the organophosphorus pesticide triazophos, which has high sensitivity and accuracy, but takes a long time and requires large-scale equipment. In order to improve the detection efficiency, various methods including chemiluminescence, fluorescence, and electrochemistry have been developed. [0003] Triazophos has inhibitory effect on acetylcholinesterase, and the biosensor based on acetylcholinesterase has fa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/48
CPCG01N27/3277G01N27/3278G01N27/48
Inventor 郁惠珍朱志强苗向阳
Owner SUZHOU CHIEN SHIUNG INST OF TECH
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