41 results about "Goby" patented technology

A fishing lure that has realistic motion when retrieved from a cast is presented. The lure has three sections: a head section, a tail section, and a mid-section. The head and tail sections are formed to resemble the head and tail areas of live bait. The mid-section has a series of segments that are joined by connectors that allow the segments to move back and forth when water impacts on the sidewalls of the segments. The overall shape of the fishing lure in one embodiment is a shad baitfish. Other embodiments include the overall shape of a lizard, a waterdog salamander, a blue gill fish, a goby fish, or a snake or worm.

The present invention discloses a marble goby compound feed containing fermented silkworm chrysalis and a use thereof. The compound feed does not contain fish meal, and comprises fermented silkworm chrysalis, alpha-starch, wheat flour, wheat gluten flour, zeolite powder, calcium biphosphate, salt and premix for carnivorous fish. The fermented silkworm chrysalis is prepared by adding mixed meal into the silkworm chrysalis, inoculating microbe, adding carbon sources and inorganic salts, fermenting and culturing the mixture. The microbe is a mixed bacterio-liquid containing bacillus subtilis, bacillus acidi lactic and yeast. According to the present invention, the marble goby compound feed does not contain any fish meal and fish meat products, is produced from fermented silkworm chrysalis, can completely replace pure live fish and ice fish for breeding, increases survival rate and growth rate of marble goby, improves quality of fish meat, significantly increases the economic benefits of aquaculture, and promotes the sustained, healthy and stable development of marble goby aquaculture.

The invention belongs to the field of DNA markers in the field of aquaculture science and in particular relates to primers and a method for distinguishing Thai marble goby (oxyeleotrismarmoratus) fries from local marble goby (odontobutispotamophila) fries by adopting microsatellite markers. A pair of primer sequences is shown in SEQIDNo.8 and SEQIDNo.9. The method comprises the following steps of: carrying out PCR (polymerase chain reaction) amplification on the samples to be distinguished by utilizing primers; and then detecting the PCR products by using 1% of agarose gel electrophoresis and carrying out EB (ethidium bromide) staining, wherein the samples with 250bp stripes are Thai marble goby and the samples with 300bp stripes are local marble goby. The method has the following beneficial effects: the microsatellite markers obtained by the method can carry out stable amplification in oxyeleotrismarmoratus and odontobutispotamophila and have stable results and clear stripes; and the method is simple and convenient to operate and is low in cost and strong in practicability.

The invention discloses a method for separating and culturing fish high-fat storage hepatocytes. a. Select 45-55d juvenile gobies, obtain their liver tissue under aseptic conditions, disperse the liver tissue into pieces, and obtain tissue pieces; b. Place a cell sieve with a pore size of 20 μm in trypsin stop solution, and A cell sieve with a pore size of 50 μm is set above the cell sieve with a pore size of 20 μm; c. Place the tissue block in the cell sieve with a pore size of 50 μm, and continuously add trypsin solution to the cell sieve with a pore size of 50 μm to digest the tissue block, flow and continue to digest, and collect at The cells on the cell sieve with a pore size of 20 μm were washed with 10% FBS to obtain high-fat storage hepatocytes; d, the high-fat storage hepatocytes were inoculated into the medium for culture. The high-fat storage hepatocytes of the present invention can be stable for one week after adhering to the wall, and the adipocytes can survive for about one month, during which they can be used for experiments at the cell level, thereby solving the problem of difficulty in separating and culturing fish mature adipocytes.

The invention belongs to the field of fish mitochondrial genome research, and specifically relates to a COⅢ and ND3 gene amplification primer, which is composed of two single-stranded oligonucleotide chains, wherein the light chain primer is the nucleotide shown in SEQ ID No.1 sequence; the heavy chain primer is the nucleotide sequence shown in SEQ ID No.2. The invention also provides the design and amplification method of the amplification primer. The invention can efficiently and specifically amplify the mitochondrial COⅢ and ND3 gene sequences of various gobies, and can be applied to the evolution and classification research of different taxonomic stages of the gobies.

The present invention relates to the field of molecular biology technology, in particular to a primer for the full sequence of the mitochondrial genome of the mullet goby and a method for designing and amplifying the full sequence of the phylogenetic analysis, including twelve pairs of primers as shown in SEQ ID NO.1-24 . The present invention solves the problem that the complete mitochondrial genome sequence of the multiscaled mullet goby cannot be amplified at present, and has (1) its mitochondrial genome can be amplified at one time, and its complete genome sequence can be quickly obtained through sequencing splicing, which is convenient and efficient Advantages; (2) Provide a primer for the complete mitochondrial genome sequence of the multiscale mullet goby, which saves time and effort, and saves reagent costs; (3) The provided systematic analysis method for the full sequence primer of the multiscale mullet goby mitochondrial genome is multiscale The population genetics, germplasm resource protection and sustainable development and utilization of mullet gobies, and the phylogenetic research of gobies provide important molecular methods and data accumulation.

The invention relates to a method for ecologically preventing and treating epizootic ulcerative syndromes of fishes, in particular to a method for ecologically preventing and treating epizootic ulcerative syndromes of fishes, particularly hybrid northern snakeheads or marble gobies (marbled sand gobies). The method includes culturing sick fishes in water with biological floccules. The method has the advantages that as proved by repeated experiments, the epizootic ulcerative syndromes of the fishes can be effectively prevented and treated by the method, and an effective rate reaches 70%; the cost is low, environments can be protected, drugs, chemical reagents and other extra reagents can be omitted, the effective rate is high, the method is simple, and operation is easy; intake of other drugs with side effects can be prevented when the method is used for preventing and treating the epizootic ulcerative syndromes for the fishes, and accordingly the food safety can be effectively guaranteed for people.

The invention discloses a method for acquiring ecological early breeding seeds of acanthogobius ommaturus Richardson, and relates to technologies for acquiring early seeds for early breeding for acanthogobius ommaturus Richardson. The method includes selecting parent fish; controlling the breeding quantities; selecting heat-insulation sheds of ponds; cultivating the parent fish through the winters; collecting fish fries. The method is characterized in that the parent fish with the weight ranging from 100 grams to 250 grams is selected in periods from November to December, and is cultivated in the ponds for takifugu rubripes through the winters, nylon films are constructed to form heat-insulation greenhouses on the upper sides of the ponds, the areas of the ponds preferably range from 3mu to 8mu, the ratio of female parent fish to male parent fish is 1:1, 10-12 pieces of parent fish is bred in each mu of the ponds under the control, 3-5 kilograms of live exopalaemon carinicauda are placed in each mu in winter cultivation periods and are used as fodders for the parent fish, water is changed regularly, an excellent water quality condition needs to be kept, 1.2-meter water levels of the ponds are kept, and the salinity of the water in the ponds keeps ranging from 6%o to 15%o; the fish fries are sampled after 20th of March in the next year, growth conditions of the fish fries are inspected, and the fish fries are timely collected and distributed when the sizes of the growing fish fries range from 4 centimeters to 6 centimeters.