Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Goby mitochondrion ND1 gene complete sequence amplimer, design and amplification method

A technique for amplifying primers and mitochondria, applied in botany equipment and methods, biochemical equipment and methods, genetic engineering, etc., can solve problems such as amplifying the full sequence of mitochondrial ND1 gene that have never been reported, and achieve important invention value and theory Significance, strong amplification ability, wide amplification range effect

Active Publication Date: 2013-05-01
ZHEJIANG OCEAN UNIV
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no universal primers have been reported to amplify the full sequence of the mitochondrial ND1 gene, especially for gobies with a wide distribution and variety of species.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Goby mitochondrion ND1 gene complete sequence amplimer, design and amplification method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Design and synthesis of primers for full-sequence amplification of the goby mitochondrial ND1 gene:

[0029] Log in to the GenBank database on the NCBI website to search for the 16S rRNA gene and tRNA-Met gene sequences of the mitochondrial genomes of other species of gobies and related fish species that have been determined, and load the sequences into the analysis software MEGA5.0. Use the ClustalW algorithm to perform multiple sequence alignment analysis, find conservative sequences, and load the found conservative sequences into Premier Primer5.0 software in manual design mode (that is, select Low under the manual option, and the specific parameters are the default settings) The primers for the full sequence amplification of the goby mitochondrial ND1 gene were designed: the light chain primer Gobies-ND1-F (shown in SEQ ID No.1) has 19 bases: CGGAGAAATCCAGGTCAGT, located on the 16S rRNA gene; the heavy chain primer Gobies-ND1-R (shown in SEQ ID No.2) has 19 bases...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the fish mitochondrial genome research field, and concretely relates to a ND1 gene complete sequence amplimer, the gene amplimer is composed of two strips of single chain oligonucleotide chains, wherein a light chain primer is a nucleotide sequence shown in SEQ ID NO.1, and a heavy chain primer is a nucleotide sequence shown in SEQ ID NO.2. The invention also provides a design and an amplification method of the amplimer. The invention can specifically amplify a plurality of goby mitochondrion ND1 gene total sequences with high efficiency, and can be used in goby different classification category systems evolution and classification research.

Description

technical field [0001] The invention belongs to the field of fish mitochondrial genome research, in particular to a primer for efficiently amplifying the full sequence of a variety of goby mitochondrial NADH reductase complex subunits 1 (NADH dehydrogenase subunits 1; ND1) genes and its design and Amplification method. Background technique [0002] The fish mitochondrial genome consists of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes (12S rRNA and 16S rRNA), a total of 37 coding genes and a major non-coding region (control region). It has the characteristics of large number, strict maternal inheritance, almost no recombination, high coding efficiency, fast evolution rate and differences in evolution rate in different regions. Due to these characteristics of the fish mitochondrial genome, it has become an important molecular marker in the research fields of fish molecular population genetics, phylogeny and conservation biology. [0003] Gobies belong to the order Pe...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12N15/53C12N15/10
Inventor 徐田军孙悦娜王日昕金逍逍
Owner ZHEJIANG OCEAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com