The invention provides an ecological breeding method for polyculture of prussian carps, plagiognathops microlepis, silver carps and bighead carps and belongs to the technical field of aquaculture. The method includes the steps that a breeding pond is selected, the pond is disinfected, water is injected into the pond, the prussian carps, the plagiognathops microlepis, the silver carps and the bighead carps are put in the pond according to the stocking size and stocking amount requirements, feeding management is carried out, after fry are put in the pond for 3 days, feed is fed, feeding is carried out 1-2 times every day, after the fishes are fed to establish conditioned reflex, feeding is carried out 3-4 times every day, water spinach is cultivated on a floating bed, the plant distance between the water spinach is 10 cm, the line spacing between the water spinach is 10 cm, and the covering area of the floating bed in the pond is 20% to 30%. Compared with existing prussian carp pond culture, the breeding method that main breeding of the prussian carps and inter breeding of the plagiognathops microlepis, the silver carps and the bighead carps are combined is adopted, the survival rate is increased, fish diseases are avoided, and a large amount of fishery medicine cost is reduced. The water spinach cultivated on the floating bed has the effect of purifying water, and economic benefits are increased.