Compsns. and methods for treating cells having double minute DNA

一种细胞、诱导细胞的技术，应用在重组DNA技术、生物化学设备和方法、含有效成分的医用配制品等方向

Inactive Publication Date: 2002-12-04
NEW BIOTECHNOLOGY CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has not been determined whether the positions occupied by acentromere DM DNA during interphase differ from the chromosomes and whether this would allow their removal from the nucleus by a budding process similar to that observed for abnormally long chromosomes ( Ruddle (1962); Jackson and Clement (1974); Lo and Fraccaro (1974); Miele, et al. (1989) and Toledo, et al. (1992))

Method used

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  • Compsns. and methods for treating cells having double minute DNA
  • Compsns. and methods for treating cells having double minute DNA
  • Compsns. and methods for treating cells having double minute DNA

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Embodiment Construction

[0026] Embodiment of the invention

[0027] The present invention finds that autonomously replicating acentric extrachromosomal structures called double minichromosomes (DMs) often mediate oncogene amplification in human tumors. According to the present inventors, removal of DMs from the nucleus by micronucleus formation initiated by budding of the nuclear envelope in S phase promotes cell maturation, differentiation and death.

[0028] Accordingly, the present invention provides a screening method for identifying potential therapeutic agents and a method for inducing appropriate cell maturation or death. Suitable test cells or cells for use in the practice of the invention include those cells which have DM or extra chromosomal DNA and are capable of eliminating this DNA by the process of micronucleus formation. These cells include, but are not limited to, prokaryotic cells such as bacterial cells or yeast cells. Also included are eukaryotic cells, such as...

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Abstract

The present invention provides methods for screening test substances by which double minutes (DM) or extrachromosomal DNA that inhibit, enhance or eliminate micronucleation formation in cells are screened. The present invention also provides a method for inducing maturation or death of cells capable of producing micronuclei. The invention also provides methods of treating diseased subjects with cells associated with the disease having DM and extra chromosomal DNA and capable of producing micronuclei to capture them. Further provided is a method of detecting chromosomal and extra chromosomal DNA in a cell.

Description

[0001] Statement of Invention Rights for Federally Sponsored Research Projects [0002] This invention was made with Government support under Grant No. DAMD17-94-J4359 by the United States Department of the Army. Accordingly, the US Government has certain rights in this invention. technical field [0003] In general, the present invention relates to screening methods for novel therapeutic drugs for treating tumor diseases such as cancer and the application of the drugs in cancer treatment. Background of the invention [0004] Publications cited throughout this application, all citations in their bibliography can be found in the text of the application or at the end of the specification immediately preceding the claims. In order to more fully describe the state of the art to which this invention pertains, the disclosures of these publications and published patent specifications, books, and issued patents are hereby incorpo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61K48/00A61K49/00A61P35/00A61P43/00C07K14/00C12N15/09C12Q1/02C12Q1/68G01N33/15G01N33/50G01N33/52G01N33/574G01N33/58G01N33/68
CPCC12Q1/68G01N33/5011G01N33/6875G01N2333/43595A61P35/00A61P43/00
Inventor G·M·瓦尔H·M·舍帕德N·施米朱特鲁·坎达
Owner NEW BIOTECHNOLOGY CO LTD
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