Method for detecting water quality genetic toxicity by broad bean root tip micronucleus test

A micronucleus test and root tip technology is applied in the field of water quality genotoxicity detection by broad bean root tip micronucleus test, which can solve the problem of invisibility of toxicity reaction, and achieve the effects of intuitive detection results, easy unification of detection results, and accurate water quality toxicity.

Inactive Publication Date: 2011-06-15
济南市供排水监测中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Disadvantages of existing evaluation technologies: most micronucleus tests only use pollution index to quantitatively evaluate water quality toxicity, the toxicity response is not intuitive enough, and the classification of toxicity grades has a certain degree of subjectivity

Method used

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  • Method for detecting water quality genetic toxicity by broad bean root tip micronucleus test
  • Method for detecting water quality genetic toxicity by broad bean root tip micronucleus test

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] This specific embodiment adopts the following steps:

[0025] (1) Choose Songzi green skin beans as indicator creatures;

[0026] (2) After soaking seeds for 24h and accelerating germination for 60h, after the primary roots of the seeds grow to 1.5cm, 6+ Take 5 concentration points in the range of 0-0.015mg / L to treat the root tip for 6 hours respectively. The Cr concentration of these 5 concentration points 6+ The concentrations are respectively 0mg / L, 0.025mg / L, 0.05mg / L, 0.1mg / L, 0.15mg / L; after soaking and washing the treated seeds with deionized water, the root tip cells are repaired for 24 hours, and then the root tip A 1cm root was cut from the tip, fixed with Carnot's fixative for 24h, and finally stained with Fulgen staining method for 5h, then the root tip cells were examined under a biological microscope, Cr 6+ The cell micronucleus per thousand percentages obtained from small to large concentrations are 0.25‰, 4.25‰, 7.75‰, 10.33‰, 12.67‰;

[0027] (3) With Cr 6+ T...

Embodiment 2

[0030] This specific embodiment adopts the following steps:

[0031] (1) Choose Songzi green skin beans as indicator creatures;

[0032] (2) After soaking seeds for 36h and accelerating germination for 48h, after the primary roots of the seeds grow to 2cm, 6+ Take 5 concentration points within the range of 0-0.015mg / L to treat the root tip for 5h respectively, and the Cr of these 5 concentration points 6+ The concentrations were 0mg / L, 0.025mg / L, 0.05mg / L, 0.1mg / L, 0.15mg / L; the treated seeds were soaked and washed with deionized water to repair the root tip cells for 22 hours, and then the root tip Cut 1cm roots from the tip and fix them with Carnot's fixative for 48h. Finally, after staining with Fulgen staining method for 6h, the root tip cells are examined under a biological microscope. 6+ The cell micronucleus per thousand fractions obtained from small to large concentrations are 0.25‰, 4.26‰, 7.78‰, 10.38‰, 12.70‰;

[0033] (3) With Cr 6+ The concentration is the abscissa, the ...

Embodiment 3

[0036] This specific embodiment adopts the following steps:

[0037] (1) Choose Songzi green skin beans as indicator creatures;

[0038] (2) After soaking seeds for 24h and accelerating germination for 50-72h, after the primary roots of the seeds grow to 1.5cm, 6+ Take 5 concentration points in the range of 0-0.015mg / L to treat the root tip for 6 hours respectively. The Cr concentration of these 5 concentration points 6+ The concentrations are respectively 0mg / L, 0.025mg / L, 0.05mg / L, 0.1mg / L, 0.15mg / L; after soaking and washing the treated seeds with deionized water, the root tip cells are repaired for 24 hours, and then the root tip A 1cm root was cut from the tip, fixed with Carnot's fixative for 24h, and finally stained with Fulgen staining method for 5h, then the root tip cells were examined under a biological microscope, Cr 6+ The cell micronucleus per thousand percentages obtained from small to large concentrations are 0.25‰, 4.26‰, 7.77‰, 10.35‰, 12.69‰;

[0039] (3) With Cr 6...

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Abstract

The invention discloses a method for detecting water quality genetic toxicity by a broad bean root tip micronucleus test, comprising the following steps of: soaking seeds for Songrun green-peel beans, accelerating germination, and obtaining a seed of which the primary root has the length of 1.5-2cm; respectively processing seed root tips by Cr6+ solutions of different concentrations; repairing, fixing and dyeing root tip cells; carrying out microscopic examination to the root tips, and calculating the permillage of cell micronucleus processed by the Cr6+ solutions of different concentrations; drawing the standard curve for the permillage of cell micronucleus relative to Cr6+ concentration; then, measuring the permillage of cell micronucleus of a water sample to be detected with the same method; and substituting the standard curve to obtain the water quality toxicity of the water sample to be detected, which is represented by Cr6+ concentration. In the method, Cr6+ is selected as the standard toxic substance, and the quantitative evaluation of the water quality toxicity is realized by the standard curve of the micronucleus permillage relative to Cr+6. The genetic toxicity of the water quality can be more intuitively and accurately detected by the method, results are easy to unify, and the repeatability is good.

Description

technical field [0001] The invention relates to a method for detecting water quality toxicity, in particular to a method for detecting water quality genotoxicity by broad bean root tip micronucleus test. Background technique [0002] In order to ensure drinking water safety and reduce the threat of environmental degradation to drinking water quality, people often use various methods to evaluate water quality to effectively grasp the health risks caused by drinking water. [0003] Micronucleus test is one of the important methods for detecting chromosomal aberrations. Since it was established by Degressi et al. Mutagenicity testing of pollutants. As early as 1986, the National Environmental Protection Agency included the broad bean root tip micronucleus test technology in the "Environmental Testing Technical Specifications" for water environment testing technology. Studies have shown that the results of micronucleus experiments have a certain correlation with the Ames test,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02
Inventor 贾瑞宝宋艳孙韶华张诺
Owner 济南市供排水监测中心
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