Experimental method of micronucleus effect on vicia faba root tip cells by nano zinc oxide
A technology of nano-zinc oxide and experimental method, which is applied in the experimental field of the micronucleus effect of nano-zinc oxide on broad bean root tip cells, to achieve the effect of strong dye toxicity and strong mutagenic effect
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[0030] 1. Materials and methods
[0031] 1.1. Experimental object: broad bean (vicia faba).
[0032] 1.2. Reagents: nanometer zinc oxide (50-80nm), 37% concentrated hydrochloric acid, absolute ethanol, glacial acetic acid, potassium dichromate, all of analytical grade.
[0033] 1.3. Instruments: artificial climate box, shaking table, electric heating constant temperature box, electric heating constant temperature blast drying oven; electronic balance; electric heating pressure sterilizer; water purifier.
[0034] 1.4. Experimental methods, such as figure 1 shown;
[0035] 1.4.1. Seed soaking and accelerating germination: select broad beans with full grain and uniform size, put them in a beaker, soak at 23°C for 36 hours, and change the water twice. After imbibition, put broad beans in an enamel dish covered with gauze, cultivate at 23°C until the root length is about 0.5cm, and change the water every 24h.
[0036] 1.4.2. Preparation of poisoning solution: Potassium dichrom...
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