Experimental method of micronucleus effect on vicia faba root tip cells by nano zinc oxide

A technology of nano-zinc oxide and experimental method, which is applied in the experimental field of the micronucleus effect of nano-zinc oxide on broad bean root tip cells, to achieve the effect of strong dye toxicity and strong mutagenic effect

Inactive Publication Date: 2010-06-30
SHANGHAI QIBAO HIGH SCHOOL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

"But because nano-zinc oxide has a broad market and attractive application prospects, and is applied to cosmetics, textiles and other products that are in direct contact with the human body, its possible negative effects on organisms are even more important for its research. sex and urgency

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  • Experimental method of micronucleus effect on vicia faba root tip cells by nano zinc oxide
  • Experimental method of micronucleus effect on vicia faba root tip cells by nano zinc oxide
  • Experimental method of micronucleus effect on vicia faba root tip cells by nano zinc oxide

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Experimental program
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Embodiment Construction

[0030] 1. Materials and methods

[0031] 1.1. Experimental object: broad bean (vicia faba).

[0032] 1.2. Reagents: nanometer zinc oxide (50-80nm), 37% concentrated hydrochloric acid, absolute ethanol, glacial acetic acid, potassium dichromate, all of analytical grade.

[0033] 1.3. Instruments: artificial climate box, shaking table, electric heating constant temperature box, electric heating constant temperature blast drying oven; electronic balance; electric heating pressure sterilizer; water purifier.

[0034] 1.4. Experimental methods, such as figure 1 shown;

[0035] 1.4.1. Seed soaking and accelerating germination: select broad beans with full grain and uniform size, put them in a beaker, soak at 23°C for 36 hours, and change the water twice. After imbibition, put broad beans in an enamel dish covered with gauze, cultivate at 23°C until the root length is about 0.5cm, and change the water every 24h.

[0036] 1.4.2. Preparation of poisoning solution: Potassium dichrom...

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Abstract

The invention relates to an experimental method of micronucleus effect on vicia faba root tip cells by nano zinc oxide, sequentially comprising the following steps: a. soaking seed and accelerating germination; b. preparing contamination liquid; c. carrying out contamination; d. recovering for cultivation; e. fixing for dissociation; and f. carrying out microscopic examination and statistics. The experimental method shows that the nano zinc oxide has strong mutational effect and has strong contamination property to the vicia faba so as to provide references for the safe application of the nano zinc oxide.

Description

technical field [0001] The invention relates to an experimental method related to the toxicity of nanometer materials, in particular to an experimental method for the micronucleus effect of nanometer zinc oxide on faba bean root tip cells. Background technique [0002] Nanotechnology is a major discovery in modern material science, and it has gradually penetrated into various fields. Nano-zinc oxide is one of the hot spots in the research and application of nano-materials. It is called "a new type of functional material facing the 21st century". Countries all over the world have invested huge sums of money in research and development. It is a major topic of my country's "863" plan. Due to its surface and interface effects, quantum size effects, volume effects and macroscopic quantum tunneling effects, as well as high transparency and excellent chemical and physical properties, it is widely used in rubber, coatings, plastics, ceramics, catalysts, chemical fibers, electronics,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/48G01N21/84C12Q1/02
Inventor 曹丽娟
Owner SHANGHAI QIBAO HIGH SCHOOL
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